6x6u - Revision history

OCA at 10:19, 22 May 2024

OCA — Wed, 22 May 2024 10:19:52 GMT

←Older revision		Revision as of 10:19, 22 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6x6u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_COM1 Pyrococcus furiosus COM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X6U FirstGlance]. <br>		<table><tr><td colspan='2'>[[6x6u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_COM1 Pyrococcus furiosus COM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X6U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PTE:TUNGSTOPTERIN+COFACTOR'>PTE</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.944Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PTE:TUNGSTOPTERIN+COFACTOR'>PTE</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6u OCA], [https://pdbe.org/6x6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6u RCSB], [https://www.ebi.ac.uk/pdbsum/6x6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6u OCA], [https://pdbe.org/6x6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6u RCSB], [https://www.ebi.ac.uk/pdbsum/6x6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6u ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:27, 7 December 2022

OCA — Wed, 07 Dec 2022 08:27:45 GMT

←Older revision		Revision as of 08:27, 7 December 2022
Line 1:		Line 1:

-	====	+	==WOR5 from Pyrococcus furiosus, taurine-bound==
-	<StructureSection load='6x6u' size='340' side='right'caption='[[6x6u]]' scene=''>	+	<StructureSection load='6x6u' size='340' side='right'caption='[[6x6u]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x6u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_COM1 Pyrococcus furiosus COM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X6U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6u OCA], [https://pdbe.org/6x6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6u RCSB], [https://www.ebi.ac.uk/pdbsum/6x6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6u ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PTE:TUNGSTOPTERIN+COFACTOR'>PTE</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6u OCA], [https://pdbe.org/6x6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6u RCSB], [https://www.ebi.ac.uk/pdbsum/6x6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/I6V2C3_9EURY I6V2C3_9EURY]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Five tungstopterin-containing oxidoreductases were characterized from the hyperthermophile Pyrococcus furiosus. Each enzyme catalyzes the reversible conversion of one or more aldehydes to the corresponding carboxylic acid, but they have different specificities. The physiological functions of only two of these enzymes are known: one, termed GAPOR, is a glycolytic enzyme that oxidizes glyceraldehyde-3-phosphate, while the other, termed AOR, oxidizes multiple aldehydes generated during peptide fermentation. Two of the enzymes have known structures (AOR and FOR). Herein, we focus on WOR5, the fifth tungstopterin enzyme to be discovered in P. furiosus. Expression of WOR5 was previously shown to be increased during cold shock (growth at 72 â), although the physiological substrate is not known. To gain insight into WOR5 function, we sought to determine both its structure and identify its intracellular substrate. Crystallization experiments were performed with a concentrated cytoplasmic extract of P. furiosus grown at 72 â and the structure of WOR5 was deduced from the crystals that were obtained. In contrast to a previous report, WOR5 is heterodimeric containing an additional polyferredoxin-like subunit with four [4Fe-4S] clusters. The active site structure of WOR5 is substantially different from that of AOR and FOR and the significant electron density observed adjacent to the tungsten cofactor of WOR5 was modeled as an aliphatic sulfonate. Biochemical assays and product analysis confirmed that WOR5 is an aliphatic sulfonate ferredoxin oxidoreductase (ASOR). A catalytic mechanism for ASOR is proposed based on the structural information and the potential role of ASOR in the cold-shock response is discussed.
		+
		+	An unprecedented function for a tungsten-containing oxidoreductase.,Mathew LG, Haja DK, Pritchett C, McCormick W, Zeineddine R, Fontenot LS, Rivera ME, Glushka J, Adams MWW, Lanzilotta WN J Biol Inorg Chem. 2022 Dec;27(8):747-758. doi: 10.1007/s00775-022-01965-0. Epub , 2022 Oct 21. PMID:36269456<ref>PMID:36269456</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x6u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pyrococcus furiosus COM1]]
		+	[[Category: Lanzilotta WN]]
		+	[[Category: Mathew LG]]

OCA at 16:45, 15 December 2021

OCA — Wed, 15 Dec 2021 16:45:57 GMT

←Older revision		Revision as of 16:45, 15 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6x6u ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6x6u' size='340' side='right'caption='[[6x6u]]' scene=''>
-	~~Authors~~: ~~Lanzilotta, W~~.N.~~, Mathew, L~~.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~WOR5 from Pyrococcus furiosus~~, ~~taurine~~-~~bound~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6u OCA], [https://pdbe.org/6x6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6u RCSB], [https://www.ebi.ac.uk/pdbsum/6x6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6u ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Lanzilotta, W.N~~]]	+	__TOC__
-	[[Category: ~~Mathew, L.G~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:41, 8 July 2020

OCA — Wed, 08 Jul 2020 10:41:42 GMT

←Older revision		Revision as of 10:41, 8 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6x6u is ON HOLD	+	The entry 6x6u is ON HOLD until Paper Publication

	Authors: Lanzilotta, W.N., Mathew, L.G.		Authors: Lanzilotta, W.N., Mathew, L.G.

OCA: Protected "6x6u" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 04:56:13 GMT

Protected "6x6u" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6x6u is ON HOLD Authors: Lanzilotta, W.N., Mathew, L.G. Description: WOR5 from Pyrococcus furiosus, taurine-bound [[Category: Unreleased Structures...

OCA — Wed, 10 Jun 2020 04:56:12 GMT

New page: '''Unreleased structure''' The entry 6x6u is ON HOLD Authors: Lanzilotta, W.N., Mathew, L.G. Description: WOR5 from Pyrococcus furiosus, taurine-bound [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 6x6u is ON HOLD

Authors: Lanzilotta, W.N., Mathew, L.G.

Description: WOR5 from Pyrococcus furiosus, taurine-bound
[[Category: Unreleased Structures]]
[[Category: Lanzilotta, W.N]]
[[Category: Mathew, L.G]]