6x80 - Revision history

OCA at 11:44, 27 April 2022

OCA — Wed, 27 Apr 2022 11:44:41 GMT

←Older revision		Revision as of 11:44, 27 April 2022
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Egelman, E H]]		[[Category: Egelman, E H]]
-	[[Category: ~~Mark~~, M A]]	+	[[Category: Kreutzberger, M A.B]]
	[[Category: Wang, F]]		[[Category: Wang, F]]
	[[Category: Bacterial flagellar filament]]		[[Category: Bacterial flagellar filament]]
	[[Category: Helical symmetry]]		[[Category: Helical symmetry]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

OCA at 05:48, 6 October 2021

OCA — Wed, 06 Oct 2021 05:48:18 GMT

←Older revision		Revision as of 05:48, 6 October 2021
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	<StructureSection load='6x80' size='340' side='right'caption='[[6x80]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='6x80' size='340' side='right'caption='[[6x80]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6x80]] is a 22 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X80 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6X80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x80]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X80 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P8E:5,7-diamino-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-non-2-ulopyranosonic+acid'>P8E</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P8E:5,7-diamino-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-non-2-ulopyranosonic+acid'>P8E</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flaA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flaA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x80 OCA], [~~http~~://pdbe.org/6x80 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6x80 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6x80 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6x80 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x80 OCA], [https://pdbe.org/6x80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x80 RCSB], [https://www.ebi.ac.uk/pdbsum/6x80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FLA_CAMJ8 FLA_CAMJ8]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.	+	[[https://www.uniprot.org/uniprot/FLA_CAMJ8 FLA_CAMJ8]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vertebrates, from zebra fish to humans, have an innate immune recognition of many bacterial flagellins. This involves a conserved eight-amino acid epitope in flagellin recognized by the Toll-like receptor 5 (TLR5). Several important human pathogens, such as Helicobacter pylori and Campylobacter jejuni, have escaped TLR5 activation by mutations in this epitope. When such mutations were introduced into Salmonella flagellin, motility was abolished. It was previously argued, using very low-resolution cryoelectron microscopy (cryo-EM), that C. jejuni accommodated these mutations by forming filaments with 7 protofilaments, rather than the 11 found in other bacteria. We have now determined the atomic structure of the C. jejuni G508A flagellar filament from a 3.5-A-resolution cryo-EM reconstruction, and show that it has 11 protofilaments. The residues in the C. jejuni TLR5 epitope have reduced contacts with the adjacent subunit compared to other bacterial flagellar filament structures. The weakening of the subunit-subunit interface introduced by the mutations in the TLR5 epitope is compensated for by extensive interactions between the outer domains of the flagellin subunits. In other bacteria, these outer domains can be nearly absent or removed without affecting motility. Furthermore, we provide evidence for the stabilization of these outer domain interactions through glycosylation of key residues. These results explain the essential role of glycosylation in C. jejuni motility, and show how the outer domains have evolved to play a role not previously found in other bacteria.
		+
		+	Atomic structure of the Campylobacter jejuni flagellar filament reveals how epsilon Proteobacteria escaped Toll-like receptor 5 surveillance.,Kreutzberger MAB, Ewing C, Poly F, Wang F, Egelman EH Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):16985-16991. doi:, 10.1073/pnas.2010996117. Epub 2020 Jul 8. PMID:32641510<ref>PMID:32641510</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x80" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellin 3D structures\|Flagellin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:38, 29 July 2020

OCA — Wed, 29 Jul 2020 11:38:54 GMT

←Older revision		Revision as of 11:38, 29 July 2020
Line 1:		Line 1:

	==Structure of the Campylobacter jejuni G508A Flagellar Filament==		==Structure of the Campylobacter jejuni G508A Flagellar Filament==
-	<StructureSection load='6x80' size='340' side='right'caption='[[6x80]]' scene=''>	+	<StructureSection load='6x80' size='340' side='right'caption='[[6x80]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X80 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x80]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X80 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X80 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x80 OCA], [http://pdbe.org/6x80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x80 RCSB], [http://www.ebi.ac.uk/pdbsum/6x80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x80 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P8E:5,7-diamino-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-non-2-ulopyranosonic+acid'>P8E</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x80 OCA], [http://pdbe.org/6x80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x80 RCSB], [http://www.ebi.ac.uk/pdbsum/6x80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x80 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FLA_CAMJ8 FLA_CAMJ8]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Egelman EH]]	+	[[Category: Egelman, E H]]
-	[[Category: Mark MA]]	+	[[Category: Mark, M A]]
-	[[Category: Wang F]]	+	[[Category: Wang, F]]
		+	[[Category: Bacterial flagellar filament]]
		+	[[Category: Helical symmetry]]
		+	[[Category: Structural protein]]

OCA at 14:10, 8 July 2020

OCA — Wed, 08 Jul 2020 14:10:21 GMT

←Older revision		Revision as of 14:10, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6x80 ~~is ON HOLD~~	+	==Structure of the Campylobacter jejuni G508A Flagellar Filament==
-		+	<StructureSection load='6x80' size='340' side='right'caption='[[6x80]]' scene=''>
-	~~Authors~~: ~~Mark~~, M.A., ~~Wang~~, F., ~~Egelman~~, E.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X80 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X80 FirstGlance]. <br>
-	~~Description: Structure of the Campylobacter jejuni G508A Flagellar Filament~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x80 OCA], [http://pdbe.org/6x80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x80 RCSB], [http://www.ebi.ac.uk/pdbsum/6x80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x80 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Egelman~~, E.H~~]]	+	__TOC__
-	[[Category: ~~Wang, F~~]]	+	</StructureSection>
-	[[Category: ~~Mark, M.A~~]]	+	[[Category: Large Structures]]
		+	[[Category: Egelman EH]]
		+	[[Category: Mark MA]]
		+	[[Category: Wang F]]

OCA: Protected "6x80" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 04:58:13 GMT

Protected "6x80" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6x80 is ON HOLD Authors: Mark, M.A., Wang, F., Egelman, E.H. Description: Structure of the Campylobacter jejuni G508A Flagellar Filament [[Category...

OCA — Wed, 10 Jun 2020 04:58:11 GMT

New page: '''Unreleased structure''' The entry 6x80 is ON HOLD Authors: Mark, M.A., Wang, F., Egelman, E.H. Description: Structure of the Campylobacter jejuni G508A Flagellar Filament [[Category...

New page

'''Unreleased structure'''

The entry 6x80 is ON HOLD

Authors: Mark, M.A., Wang, F., Egelman, E.H.

Description: Structure of the Campylobacter jejuni G508A Flagellar Filament
[[Category: Unreleased Structures]]
[[Category: Egelman, E.H]]
[[Category: Wang, F]]
[[Category: Mark, M.A]]