6x9p - Revision history

OCA at 14:47, 18 October 2023

OCA — Wed, 18 Oct 2023 14:47:40 GMT

←Older revision		Revision as of 14:47, 18 October 2023
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	==2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain==		==2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain==
-	<StructureSection load='6x9p' size='340' side='right'caption='[[6x9p]]' scene=''>	+	<StructureSection load='6x9p' size='340' side='right'caption='[[6x9p]], [[Resolution\|resolution]] 1.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinomonas_primoryensis Marinomonas primoryensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X9P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x9p OCA], [https://pdbe.org/6x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x9p RCSB], [https://www.ebi.ac.uk/pdbsum/6x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x9p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UZJ:2-deoxy-beta-D-ribopyranose'>UZJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x9p OCA], [https://pdbe.org/6x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x9p RCSB], [https://www.ebi.ac.uk/pdbsum/6x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x9p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A1YIY3_9GAMM A1YIY3_9GAMM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carbohydrate recognition by lectins governs critical host-microbe interactions. MpPA14 (Marinomonas primoryensis PA14 domain) lectin is a domain of a 1.5-MDa adhesin responsible for a symbiotic bacterium-diatom interaction in Antarctica. Here, we show that MpPA14 binds various monosaccharides, with l-fucose and N-acetylglucosamine being the strongest ligands (dissociation constant [Kd ], approximately 150 muM). High-resolution structures of MpPA14 with 15 different sugars bound elucidated the molecular basis for the lectin's apparent binding promiscuity but underlying selectivity. MpPA14 mediates strong Ca(2+)-dependent interactions with the 3,4-diols of l-fucopyranose and glucopyranoses, and it binds other sugars via their specific minor isomers. Thus, MpPA14 only binds polysaccharides like branched glucans and fucoidans with these free end groups. Consistent with our findings, adhesion of MpPA14 to diatom cells was selectively blocked by l-fucose, but not by N-acetyl galactosamine. The MpPA14 lectin homolog present in a Vibrio cholerae adhesin was produced and was shown to have the same sugar binding preferences as MpPA14. The pathogen's lectin was unable to effectively bind the diatom in the presence of fucose, thus demonstrating the antiadhesion strategy of blocking infection via ligand-based antagonists.IMPORTANCE Bacterial adhesins are key virulence factors that are essential for the pathogen-host interaction and biofilm formation that cause most infections. Many of the adhesin-driven cell-cell interactions are mediated by lectins. Our study reveals for the first time the molecular basis underlying the binding selectivity of a common bacterial adhesin lectin from the marine bacterium Marinomonas primoryensis, homologs of which are found in both environmental and pathogenic species. The lectin-ligand interactions illustrated at the atomic level guided the identification of a ligand that serves as an inhibitor to block bacterium-host adhesion. With conventional bactericidal antibiotics losing their potency due to resistance, our work gives critical insight into an antiadhesion strategy to treat bacterial infections.
		+
		+	Structural Basis of Ligand Selectivity by a Bacterial Adhesin Lectin Involved in Multispecies Biofilm Formation.,Guo S, Vance TDR, Zahiri H, Eves R, Stevens C, Hehemann JH, Vidal-Melgosa S, Davies PL mBio. 2021 Apr 6;12(2). pii: mBio.00130-21. doi: 10.1128/mBio.00130-21. PMID:33824212<ref>PMID:33824212</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x9p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Marinomonas primoryensis]]
	[[Category: Davies PL]]		[[Category: Davies PL]]
	[[Category: Guo S]]		[[Category: Guo S]]

OCA at 14:33, 2 June 2021

OCA — Wed, 02 Jun 2021 14:33:01 GMT

←Older revision		Revision as of 14:33, 2 June 2021
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-	'''Unreleased structure'''

-	~~The entry 6x9p is ON HOLD until Paper Publication~~	+	==2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain==
-		+	<StructureSection load='6x9p' size='340' side='right'caption='[[6x9p]]' scene=''>
-	~~Authors: Guo, S., Davies, P.L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X9P FirstGlance]. <br>
-	~~Description:~~ 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x9p OCA], [https://pdbe.org/6x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x9p RCSB], [https://www.ebi.ac.uk/pdbsum/6x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x9p ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Davies~~, P.L~~]]	+	__TOC__
-	[[Category: Guo, S]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Davies PL]]
		+	[[Category: Guo S]]

OCA at 06:33, 22 July 2020

OCA — Wed, 22 Jul 2020 06:33:01 GMT

←Older revision		Revision as of 06:33, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6x9p is ON HOLD	+	The entry 6x9p is ON HOLD until Paper Publication

	Authors: Guo, S., Davies, P.L.		Authors: Guo, S., Davies, P.L.
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	Description: 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain		Description: 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Guo, S]]
	[[Category: Davies, P.L]]		[[Category: Davies, P.L]]
		+	[[Category: Guo, S]]

OCA: Protected "6x9p" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:01:22 GMT

Protected "6x9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:01, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6x9p is ON HOLD Authors: Guo, S., Davies, P.L. Description: 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding dom...

OCA — Wed, 10 Jun 2020 05:01:21 GMT

New page: '''Unreleased structure''' The entry 6x9p is ON HOLD Authors: Guo, S., Davies, P.L. Description: 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding dom...

New page

'''Unreleased structure'''

The entry 6x9p is ON HOLD

Authors: Guo, S., Davies, P.L.

Description: 2-deoxyribose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain
[[Category: Unreleased Structures]]
[[Category: Guo, S]]
[[Category: Davies, P.L]]