6xcq - Revision history

OCA at 14:50, 18 October 2023

OCA — Wed, 18 Oct 2023 14:50:29 GMT

←Older revision		Revision as of 14:50, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='6xcq' size='340' side='right'caption='[[6xcq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6xcq' size='340' side='right'caption='[[6xcq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6xcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XCQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XCQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PGO:S~~-1,2~~-PROPANEDIOL'>PGO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ereC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~573~~ "~~Bacillus pneumoniae~~" ~~(Schroeter 1886) Flugge 1886])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xcq OCA], [https://pdbe.org/6xcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xcq RCSB], [https://www.ebi.ac.uk/pdbsum/6xcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xcq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xcq OCA], [https://pdbe.org/6xcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xcq RCSB], [https://www.ebi.ac.uk/pdbsum/6xcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xcq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C7C425_KLEPN C7C425_KLEPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berghuis~~, A M~~]]	+	[[Category: Berghuis AM]]
-	[[Category: Park, J]]	+	[[Category: Park J]]
-	[[Category: Zielinski, M]]	+	[[Category: Zielinski M]]
-	~~[[Category: Esterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Macrolide~~]]	+

OCA at 10:44, 31 March 2021

OCA — Wed, 31 Mar 2021 10:44:00 GMT

←Older revision		Revision as of 10:44, 31 March 2021
Line 1:		Line 1:

	==Erythromycin esterase EreC, mutant H289N in its closed conformation==		==Erythromycin esterase EreC, mutant H289N in its closed conformation==
-	<StructureSection load='6xcq' size='340' side='right'caption='[[6xcq]]' scene=''>	+	<StructureSection load='6xcq' size='340' side='right'caption='[[6xcq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XCQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XCQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xcq OCA], [https://pdbe.org/6xcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xcq RCSB], [https://www.ebi.ac.uk/pdbsum/6xcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xcq ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ereC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xcq OCA], [https://pdbe.org/6xcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xcq RCSB], [https://www.ebi.ac.uk/pdbsum/6xcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xcq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Macrolides are a class of antibiotics widely used in both medicine and agriculture. Unsurprisingly, as a consequence of their exensive usage a plethora of resistance mechanisms have been encountered in pathogenic bacteria. One of these resistance mechanisms entails the enzymatic cleavage of the macrolides' macrolactone ring by erythromycin esterases (Eres). The most frequently identified Ere enzyme is EreA, which confers resistance to the majority of clinically used macrolides. Despite the role Eres play in macrolide resistance, research into this family enzymes has been sparse. Here, we report the first three-dimensional structures of an erythromycin esterase, EreC. EreC is an extremely close homologue of EreA, displaying more than 90% sequence identity. Two structures of this enzyme, in conjunction with in silico flexible docking studies and previously reported mutagenesis data allowed for the proposal of a detailed catalytic mechanism for the Ere family of enzymes, labeling them as metal-independent hydrolases. Also presented are substrate spectrum assays for different members of the Ere family. The results from these assays together with an examination of residue conservation for the macrolide binding site in Eres, suggests two distinct active site archetypes within the Ere enzyme family.
		+
		+	Structural and functional insights into esterase-mediated macrolide resistance.,Zielinski M, Park J, Sleno B, Berghuis AM Nat Commun. 2021 Mar 19;12(1):1732. doi: 10.1038/s41467-021-22016-3. PMID:33741980<ref>PMID:33741980</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xcq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berghuis AM]]	+	[[Category: Berghuis, A M]]
-	[[Category: Park J]]	+	[[Category: Park, J]]
-	[[Category: Zielinski M]]	+	[[Category: Zielinski, M]]
		+	[[Category: Esterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Macrolide]]

OCA at 09:57, 17 February 2021

OCA — Wed, 17 Feb 2021 09:57:01 GMT

←Older revision		Revision as of 09:57, 17 February 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6xcq is ~~ON HOLD until Paper Publication~~	+	==Erythromycin esterase EreC, mutant H289N in its closed conformation==
-		+	<StructureSection load='6xcq' size='340' side='right'caption='[[6xcq]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XCQ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xcq OCA], [https://pdbe.org/6xcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xcq RCSB], [https://www.ebi.ac.uk/pdbsum/6xcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xcq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Berghuis AM]]
		+	[[Category: Park J]]
		+	[[Category: Zielinski M]]

OCA: Protected "6xcq" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2020 08:43:19 GMT

Protected "6xcq" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 6xcq is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Jun 2020 08:43:18 GMT

New page: '''Unreleased structure''' The entry 6xcq is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xcq is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]