6xfu - Revision history

OCA at 09:16, 25 October 2023

OCA — Wed, 25 Oct 2023 09:16:53 GMT

←Older revision		Revision as of 09:16, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6xfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XFU FirstGlance]. <br>		<table><tr><td colspan='2'>[[6xfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XFU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfu OCA], [https://pdbe.org/6xfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xfu RCSB], [https://www.ebi.ac.uk/pdbsum/6xfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfu OCA], [https://pdbe.org/6xfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xfu RCSB], [https://www.ebi.ac.uk/pdbsum/6xfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfu ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:35, 7 April 2023

OCA — Fri, 07 Apr 2023 06:35:53 GMT

←Older revision		Revision as of 06:35, 7 April 2023
Line 1:		Line 1:

	==PmtCD peptide exporter basket domain==		==PmtCD peptide exporter basket domain==
-	<StructureSection load='6xfu' size='340' side='right'caption='[[6xfu]]' scene=''>	+	<StructureSection load='6xfu' size='340' side='right'caption='[[6xfu]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XFU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XFU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfu OCA], [~~http~~://pdbe.org/6xfu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xfu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xfu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfu ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfu OCA], [https://pdbe.org/6xfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xfu RCSB], [https://www.ebi.ac.uk/pdbsum/6xfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2FWW9_STAA8 Q2FWW9_STAA8]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs are amphipathic, membrane-destructive cytolytic peptides that are exported to the host-cell environment by a designated adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, the PSM transporter (PmtABCD). Here, we demonstrate that the minimal Pmt unit necessary for PSM export is PmtCD and provide its first atomic characterization by single-particle cryo-EM and x-ray crystallography. We have captured the transporter in the ATP-bound state at near atomic resolution, revealing a type II ABC exporter fold, with an additional cytosolic domain. Comparison to a lower-resolution nucleotide-free map displaying an "open" conformation and putative hydrophobic inner chamber of a size able to accommodate the binding of two PSM peptides provides mechanistic insight and sets the foundation for therapeutic design.
		+
		+	Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins.,Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ Sci Adv. 2020 Sep 30;6(40). pii: 6/40/eabb8219. doi: 10.1126/sciadv.abb8219., Print 2020 Sep. PMID:32998902<ref>PMID:32998902</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xfu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus]]
	[[Category: Alexander JAN]]		[[Category: Alexander JAN]]
	[[Category: Strynadka NCJ]]		[[Category: Strynadka NCJ]]
	[[Category: Zeytuni N]]		[[Category: Zeytuni N]]

OCA at 05:48, 14 October 2020

OCA — Wed, 14 Oct 2020 05:48:42 GMT

←Older revision		Revision as of 05:48, 14 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6xfu is ~~ON HOLD until Paper Publication~~	+	==PmtCD peptide exporter basket domain==
-		+	<StructureSection load='6xfu' size='340' side='right'caption='[[6xfu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XFU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XFU FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xfu OCA], [http://pdbe.org/6xfu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xfu RCSB], [http://www.ebi.ac.uk/pdbsum/6xfu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xfu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Alexander JAN]]
		+	[[Category: Strynadka NCJ]]
		+	[[Category: Zeytuni N]]

OCA at 09:21, 29 July 2020

OCA — Wed, 29 Jul 2020 09:21:53 GMT

←Older revision		Revision as of 09:21, 29 July 2020
Line 3:		Line 3:
	The entry 6xfu is ON HOLD until Paper Publication		The entry 6xfu is ON HOLD until Paper Publication

-	Authors: ~~Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N.~~	+	Authors:

-	Description: ~~PmtCD peptide exporter basket domain~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Alexander, J.A.N]]
-	[[Category: Strynadka, N.C.J]]
-	[[Category: Zeytuni, N]]

OCA at 10:46, 8 July 2020

OCA — Wed, 08 Jul 2020 10:46:05 GMT

←Older revision		Revision as of 10:46, 8 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xfu is ON HOLD	+	The entry 6xfu is ON HOLD until Paper Publication

	Authors: Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N.		Authors: Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Alexander, J.A.N]]		[[Category: Alexander, J.A.N]]
-	[[Category: Zeytuni, N]]
	[[Category: Strynadka, N.C.J]]		[[Category: Strynadka, N.C.J]]
		+	[[Category: Zeytuni, N]]

OCA: Protected "6xfu" [edit=sysop:move=sysop]

OCA — Wed, 24 Jun 2020 18:29:29 GMT

Protected "6xfu" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:29, 24 June 2020

OCA: New page: '''Unreleased structure''' The entry 6xfu is ON HOLD Authors: Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N. Description: PmtCD peptide exporter basket domain [[Category: Unreleased...

OCA — Wed, 24 Jun 2020 18:29:28 GMT

New page: '''Unreleased structure''' The entry 6xfu is ON HOLD Authors: Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N. Description: PmtCD peptide exporter basket domain [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 6xfu is ON HOLD

Authors: Zeytuni, N., Strynadka, N.C.J., Alexander, J.A.N.

Description: PmtCD peptide exporter basket domain
[[Category: Unreleased Structures]]
[[Category: Alexander, J.A.N]]
[[Category: Zeytuni, N]]
[[Category: Strynadka, N.C.J]]