6xj5 - Revision history

OCA at 09:16, 25 October 2023

OCA — Wed, 25 Oct 2023 09:16:54 GMT

←Older revision		Revision as of 09:16, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6xj5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._RS-16 Pseudomonas sp. RS-16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6xj5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._RS-16 Pseudomonas sp. RS-16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=V44:S-[2,2-bis(1-methyl-1H-imidazol-2-yl)ethyl]-L-cysteine'>V44</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.11Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=V44:S-[2,2-bis(1-methyl-1H-imidazol-2-yl)ethyl]-L-cysteine'>V44</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj5 OCA], [https://pdbe.org/6xj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj5 OCA], [https://pdbe.org/6xj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:36, 19 April 2023

OCA — Wed, 19 Apr 2023 09:36:32 GMT

←Older revision		Revision as of 09:36, 19 April 2023
Line 1:		Line 1:

-	====	+	==Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design==
-	<StructureSection load='6xj5' size='340' side='right'caption='[[6xj5]]' scene=''>	+	<StructureSection load='6xj5' size='340' side='right'caption='[[6xj5]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xj5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._RS-16 Pseudomonas sp. RS-16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj5 OCA], [https://pdbe.org/6xj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj5 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=V44:S-[2,2-bis(1-methyl-1H-imidazol-2-yl)ethyl]-L-cysteine'>V44</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj5 OCA], [https://pdbe.org/6xj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CBPG_PSES6 CBPG_PSES6] Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Precise metal-protein coordination by design remains a considerable challenge. Polydentate, high-metal-affinity protein modifications, both chemical and recombinant, can enable metal localization. However, these constructs are often bulky, conformationally and stereochemically ill-defined, or coordinately saturated. Here, we expand the biomolecular metal-coordination toolbox with the irreversible attachment to cysteine of bis(1-methylimidazol-2-yl)ethene ("BMIE"), which generates a compact imidazole-based metal-coordinating ligand. Conjugate additions of small-molecule thiols (thiocresol and N-Boc-Cys) with BMIE confirm general thiol reactivity. The BMIE adducts are shown to complex the divalent metal ions Cu(++) and Zn(++) in bidentate (N(2)) and tridentate (N(2)S*) coordination geometries. Cysteine-targeted BMIE modification (>90% yield at pH 8.0) of a model protein, the S203C variant of carboxypeptidase G2 (CPG2), measured with ESI-MS, confirms its utility as a site-selective bioconjugation method. ICP-MS analysis confirms mono-metallation of the BMIE-modified CPG2 protein with Zn(++), Cu(++), and Co(++). EPR characterization of the BMIE-modified CPG2 protein reveals the structural details of the site selective 1:1 BMIE-Cu(++) coordination and symmetric tetragonal geometry under physiological conditions and in the presence of various competing and exchangeable ligands (H(2)O/HO(-), tris, and phenanthroline). An X-ray protein crystal structure of BMIE-modified CPG2-S203C demonstrates that the BMIE modification is minimally disruptive to the overall protein structure, including the carboxypeptidase active sites, although Zn(++) metalation could not be conclusively discerned at the resolution obtained. The carboxypeptidase catalytic activity of BMIE-modified CPG2-S203C was also assayed and found to be minimally affected. These features, combined with ease of attachment, define the new BMIE-based ligation as a versatile metalloprotein design tool, and enable future catalytic and structural applications.
		+
		+	A Bis(imidazole)-based cysteine labeling tool for metalloprotein assembly.,Ahmad R, Tyryshkin AM, Xie L, Hansen WA, Yachnin BJ, Emge TJ, Mashrai A, Khare SD, Knapp S J Inorg Biochem. 2023 Apr 1;244:112206. doi: 10.1016/j.jinorgbio.2023.112206. PMID:37030124<ref>PMID:37030124</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xj5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Pseudomonas sp. RS-16]]
		+	[[Category: Hansen WA]]
		+	[[Category: Khare SD]]
		+	[[Category: Yachnin BJ]]

OCA at 03:30, 2 July 2021

OCA — Fri, 02 Jul 2021 03:30:40 GMT

←Older revision		Revision as of 03:30, 2 July 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6xj5 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xj5' size='340' side='right'caption='[[6xj5]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj5 OCA], [https://pdbe.org/6xj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:49, 5 August 2020

OCA — Wed, 05 Aug 2020 03:49:27 GMT

←Older revision		Revision as of 03:49, 5 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xj5 is ON HOLD	+	The entry 6xj5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xj5" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 10:51:14 GMT

Protected "6xj5" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xj5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Jul 2020 10:51:13 GMT

New page: '''Unreleased structure''' The entry 6xj5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xj5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]