6xj7 - Revision history

OCA at 14:53, 18 October 2023

OCA — Wed, 18 Oct 2023 14:53:20 GMT

←Older revision		Revision as of 14:53, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='6xj7' size='340' side='right'caption='[[6xj7]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6xj7' size='340' side='right'caption='[[6xj7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6xj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Camjj Camjj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_81-176 Campylobacter jejuni subsp. jejuni 81-176]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ7 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CJJ81176_0915 (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=354242 CAMJJ])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj7 OCA], [https://pdbe.org/6xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj7 OCA], [https://pdbe.org/6xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3PAQ3_CAMJJ A0A0H3PAQ3_CAMJJ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camjj~~]]	+	[[Category: Campylobacter jejuni subsp. jejuni 81-176]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chan~~, A C~~]]	+	[[Category: Chan AC]]
-	[[Category: Lin~~, C S~~]]	+	[[Category: Lin CS]]
-	[[Category: Murphy~~, M E]]~~	+	[[Category: Murphy ME]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ld-carboxypeptidase]]~~	+
-	~~[[Category: Ntf2 fold]]~~	+
-	~~[[Category: Peptidase]]~~	+
-	~~[[Category: Peptidoglycan hydrolase~~]]	+

OCA at 06:50, 24 March 2021

OCA — Wed, 24 Mar 2021 06:50:48 GMT

←Older revision		Revision as of 06:50, 24 March 2021
Line 1:		Line 1:

	==Crystal structure of the helical cell shape determining protein Pgp2 (K307A mutant) from Campylobacter jejuni==		==Crystal structure of the helical cell shape determining protein Pgp2 (K307A mutant) from Campylobacter jejuni==
-	<StructureSection load='6xj7' size='340' side='right'caption='[[6xj7]]' scene=''>	+	<StructureSection load='6xj7' size='340' side='right'caption='[[6xj7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camjj Camjj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj7 OCA], [https://pdbe.org/6xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj7 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CJJ81176_0915 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354242 CAMJJ])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj7 OCA], [https://pdbe.org/6xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The helical morphology of Campylobacter jejuni, a bacterium involved in host gut colonization and pathogenesis in humans, is determined by the structure of the peptidoglycan (PG) layer. This structure is dictated by trimming of peptide stems by the LD-carboxypeptidase Pgp2 within the periplasm. The interaction interface between Pgp2 and PG to select sites for peptide trimming is unknown. We determined a 1.6 A resolution crystal structure of Pgp2, which contains a conserved LD-carboxypeptidase domain and a previously uncharacterized domain with an NTF2-like fold (NTF2). We identified a pocket in the NTF2 domain formed by conserved residues and located approximately 40 A from the LD-carboxypeptidase active site. Expression of pgp2 in trans with substitutions of charged (Lys257, Lys307, Glu324) and hydrophobic residues (Phe242 and Tyr233) within the pocket did not restore helical morphology to a pgp2 deletion strain. Muropeptide analysis indicated a decrease of murotripeptides in the deletion strain expressing these mutants, suggesting reduced Pgp2 catalytic activity. Pgp2 but not the K307A mutant was pulled down by C. jejuni Deltapgp2 PG sacculi, supporting a role for the pocket in PG binding. NMR spectroscopy was used to define the interaction interfaces of Pgp2 with several PG fragments, which bound to the active site within the LD-carboxypeptidase domain and the pocket of the NTF2 domain. We propose a model for Pgp2 binding to PG strands involving both the LD-carboxypeptidase domain and the accessory NTF2 domain to induce a helical cell shape.
		+
		+	Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs helical cell shape of Campylobacter jejuni.,Sheng-Huei Lin C, Chan ACK, Vermeulen J, Brockerman J, Soni AS, Tanner ME, Gaynor EC, McIntosh LP, Simorre JP, Murphy MEP J Biol Chem. 2021 Mar 9:100528. doi: 10.1016/j.jbc.2021.100528. PMID:33711341<ref>PMID:33711341</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xj7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camjj]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chan AC]]	+	[[Category: Chan, A C]]
-	[[Category: Lin CS]]	+	[[Category: Lin, C S]]
-	[[Category: Murphy ME]]	+	[[Category: Murphy, M E]]
		+	[[Category: Hydrolase]]
		+	[[Category: Ld-carboxypeptidase]]
		+	[[Category: Ntf2 fold]]
		+	[[Category: Peptidase]]
		+	[[Category: Peptidoglycan hydrolase]]

OCA at 06:43, 17 March 2021

OCA — Wed, 17 Mar 2021 06:43:14 GMT

←Older revision		Revision as of 06:43, 17 March 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6xj7 is ON HOLD until Paper Publication~~	+	==Crystal structure of the helical cell shape determining protein Pgp2 (K307A mutant) from Campylobacter jejuni==
-		+	<StructureSection load='6xj7' size='340' side='right'caption='[[6xj7]]' scene=''>
-	~~Authors: Lin, C.S., Chan, A.C., Murphy, M.E.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XJ7 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the helical cell shape determining protein Pgp2 (K307A mutant) from Campylobacter jejuni	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xj7 OCA], [https://pdbe.org/6xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xj7 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Lin~~, C.S]]	+	__TOC__
-	[[Category: ~~Murphy, M.E~~]]	+	</StructureSection>
-	[[Category: Chan~~, A.C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Chan AC]]
		+	[[Category: Lin CS]]
		+	[[Category: Murphy ME]]

OCA at 06:42, 22 July 2020

OCA — Wed, 22 Jul 2020 06:42:23 GMT

←Older revision		Revision as of 06:42, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xj7 is ON HOLD	+	The entry 6xj7 is ON HOLD until Paper Publication

	Authors: Lin, C.S., Chan, A.C., Murphy, M.E.		Authors: Lin, C.S., Chan, A.C., Murphy, M.E.

-	Description: Crystal ~~Structure~~ of ~~apo Cell Shape Determinant~~ protein Pgp2 from ~~Camoylobacter~~ jejuni~~, K307A mutation~~	+	Description: Crystal structure of the helical cell shape determining protein Pgp2 (K307A mutant) from Campylobacter jejuni
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lin, C.S]]		[[Category: Lin, C.S]]
	[[Category: Murphy, M.E]]		[[Category: Murphy, M.E]]
	[[Category: Chan, A.C]]		[[Category: Chan, A.C]]

OCA: Protected "6xj7" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 10:51:25 GMT

Protected "6xj7" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xj7 is ON HOLD Authors: Lin, C.S., Chan, A.C., Murphy, M.E. Description: Crystal Structure of apo Cell Shape Determinant protein Pgp2 from Camoylo...

OCA — Wed, 08 Jul 2020 10:51:24 GMT

New page: '''Unreleased structure''' The entry 6xj7 is ON HOLD Authors: Lin, C.S., Chan, A.C., Murphy, M.E. Description: Crystal Structure of apo Cell Shape Determinant protein Pgp2 from Camoylo...

New page

'''Unreleased structure'''

The entry 6xj7 is ON HOLD

Authors: Lin, C.S., Chan, A.C., Murphy, M.E.

Description: Crystal Structure of apo Cell Shape Determinant protein Pgp2 from Camoylobacter jejuni, K307A mutation
[[Category: Unreleased Structures]]
[[Category: Lin, C.S]]
[[Category: Murphy, M.E]]
[[Category: Chan, A.C]]