OCA at 10:01, 14 July 2021

OCA — Wed, 14 Jul 2021 10:01:25 GMT

←Older revision		Revision as of 10:01, 14 July 2021
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	==Structure of a mosquito complement inhibitor from Anopheles albimanus==		==Structure of a mosquito complement inhibitor from Anopheles albimanus==
-	<StructureSection load='6xke' size='340' side='right'caption='[[6xke]]' scene=''>	+	<StructureSection load='6xke' size='340' side='right'caption='[[6xke]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XKE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xke]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anoal Anoal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XKE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xke OCA], [https://pdbe.org/6xke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xke RCSB], [https://www.ebi.ac.uk/pdbsum/6xke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xke ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xke OCA], [https://pdbe.org/6xke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xke RCSB], [https://www.ebi.ac.uk/pdbsum/6xke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xke ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Inhibition of the alternative pathway (AP) of complement by saliva from Anopheles mosquitoes facilitates feeding by blocking production of the anaphylatoxins C3a and C5a which activate mast cells leading to plasma extravasation, pain and itching. We have previously shown that albicin, a member of the SG7 protein family from An. albimanus blocks the AP by binding to and inhibiting the function of the C3 convertase, C3bBb. Here we show that SG7.AF, the albicin homolog from An. freeborni, has a similar potency to albicin but is more active in the presence of properdin, a plasma protein that acts to stabilize C3bBb. Conversely, albicin is highly active in the absence or presence of properdin. Albicin and SG7.AF stabilize the C3bBb complex in a form that accumulates on surface plasmon resonance (SPR) surfaces coated with properdin but SG7.AF binds with lower affinity than albicin. Albicin induces oligomerization of the complex in solution, suggesting that it is oligomerization that leads to stabilization on SPR surfaces. Anophensin, the albicin ortholog from An. stephensi, is only weakly active as an inhibitor of the AP, suggesting that the SG7 family may play a different functional role in this species and other species of the subgenus Cellia, containing the major malaria vectors in Africa and Asia. Crystal structures of albicin and SG7.AF reveal a novel four-helix bundle arrangement, that is stabilized by a N-terminal hydrogen bonding network. These structures provide insight into the SG7 family and related mosquito salivary proteins including the platelet-inhibitory 30kDa family.
		+
		+	Salivary complement inhibitors from mosquitoes: Structure and mechanism of action.,Strayer EC, Lu S, Ribeiro JM, Andersen JF J Biol Chem. 2020 Nov 16. pii: S0021-9258(20)00071-X. doi:, 10.1074/jbc.RA120.015230. PMID:33199367<ref>PMID:33199367</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xke" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Anoal]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andersen JF]]	+	[[Category: Andersen, J F]]
-	[[Category: Strayer E]]	+	[[Category: Strayer, E]]
		+	[[Category: Alternative pathway]]
		+	[[Category: C3bbb]]
		+	[[Category: Complement]]
		+	[[Category: Inhibitor]]
		+	[[Category: Protein binding]]

OCA at 08:51, 5 May 2021

OCA — Wed, 05 May 2021 08:51:27 GMT

←Older revision		Revision as of 08:51, 5 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6xke ~~is ON HOLD until Paper Publication~~	+	==Structure of a mosquito complement inhibitor from Anopheles albimanus==
-		+	<StructureSection load='6xke' size='340' side='right'caption='[[6xke]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XKE FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xke OCA], [https://pdbe.org/6xke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xke RCSB], [https://www.ebi.ac.uk/pdbsum/6xke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xke ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Andersen JF]]
		+	[[Category: Strayer E]]

OCA at 03:50, 5 August 2020

OCA — Wed, 05 Aug 2020 03:50:11 GMT

←Older revision		Revision as of 03:50, 5 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xke is ON HOLD	+	The entry 6xke is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xke" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 10:54:15 GMT

Protected "6xke" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:54, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xke is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Jul 2020 10:54:13 GMT

New page: '''Unreleased structure''' The entry 6xke is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xke is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xke - Revision history

OCA at 10:01, 14 July 2021

OCA at 08:51, 5 May 2021

OCA at 03:50, 5 August 2020

OCA: Protected "6xke" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xke is ON HOLD Authors: Description: Category: Unreleased Structures