6xl1 - Revision history

OCA at 14:54, 18 October 2023

OCA — Wed, 18 Oct 2023 14:54:40 GMT

←Older revision		Revision as of 14:54, 18 October 2023
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-	====	+	==crystal structure of cA4-activated Card1(D294N)==
-	<StructureSection load='6xl1' size='340' side='right'caption='[[6xl1]]' scene=''>	+	<StructureSection load='6xl1' size='340' side='right'caption='[[6xl1]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xl1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_succinifaciens_DSM_2489 Treponema succinifaciens DSM 2489] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XL1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xl1 OCA], [https://pdbe.org/6xl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xl1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xl1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xl1 OCA], [https://pdbe.org/6xl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xl1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xl1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/F2NWD3_TRES6 F2NWD3_TRES6]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the type III CRISPR-Cas immune response of prokaryotes, infection triggers the production of cyclic oligoadenylates that bind and activate proteins that contain a CARF domain(1,2). Many type III loci are associated with proteins in which the CRISPR-associated Rossman fold (CARF) domain is fused to a restriction endonuclease-like domain(3,4). However, with the exception of the well-characterized Csm6 and Csx1 ribonucleases(5,6), whether and how these inducible effectors provide defence is not known. Here we investigated a type III CRISPR accessory protein, which we name cyclic-oligoadenylate-activated single-stranded ribonuclease and single-stranded deoxyribonuclease 1 (Card1). Card1 forms a symmetrical dimer that has a large central cavity between its CRISPR-associated Rossmann fold and restriction endonuclease domains that binds cyclic tetra-adenylate. The binding of ligand results in a conformational change comprising the rotation of individual monomers relative to each other to form a more compact dimeric scaffold, in which a manganese cation coordinates the catalytic residues and activates the cleavage of single-stranded-but not double-stranded-nucleic acids (both DNA and RNA). In vivo, activation of Card1 induces dormancy of the infected hosts to provide immunity against phage infection and plasmids. Our results highlight the diversity of strategies used in CRISPR systems to provide immunity.
		+
		+	The Card1 nuclease provides defence during type III CRISPR immunity.,Rostol JT, Xie W, Kuryavyi V, Maguin P, Kao K, Froom R, Patel DJ, Marraffini LA Nature. 2021 Feb;590(7847):624-629. doi: 10.1038/s41586-021-03206-x. Epub 2021, Jan 18. PMID:33461211<ref>PMID:33461211</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xl1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Treponema succinifaciens DSM 2489]]
		+	[[Category: Marraffini L]]
		+	[[Category: Patel DJ]]
		+	[[Category: Rostol J]]
		+	[[Category: Xie W]]

OCA at 11:08, 10 February 2021

OCA — Wed, 10 Feb 2021 11:08:26 GMT

←Older revision		Revision as of 11:08, 10 February 2021
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	<StructureSection load='6xl1' size='340' side='right'caption='[[6xl1]]' scene=''>		<StructureSection load='6xl1' size='340' side='right'caption='[[6xl1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xl1 OCA], [~~http~~://pdbe.org/6xl1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xl1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xl1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xl1 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xl1 OCA], [https://pdbe.org/6xl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xl1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xl1 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 06:44, 30 December 2020

OCA — Wed, 30 Dec 2020 06:44:43 GMT

←Older revision		Revision as of 06:44, 30 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xl1 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xl1' size='340' side='right'caption='[[6xl1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xl1 OCA], [http://pdbe.org/6xl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xl1 RCSB], [http://www.ebi.ac.uk/pdbsum/6xl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xl1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:50, 5 August 2020

OCA — Wed, 05 Aug 2020 03:50:29 GMT

←Older revision		Revision as of 03:50, 5 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xl1 is ON HOLD	+	The entry 6xl1 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xl1" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 10:55:47 GMT

Protected "6xl1" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:55, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xl1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 08 Jul 2020 10:55:45 GMT

New page: '''Unreleased structure''' The entry 6xl1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xl1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]