6xls - Revision history

OCA at 14:54, 18 October 2023

OCA — Wed, 18 Oct 2023 14:54:56 GMT

←Older revision		Revision as of 14:54, 18 October 2023
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	==The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272==		==The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272==
-	<StructureSection load='6xls' size='340' side='right'caption='[[6xls]]' scene=''>	+	<StructureSection load='6xls' size='340' side='right'caption='[[6xls]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XLS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xls OCA], [https://pdbe.org/6xls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xls RCSB], [https://www.ebi.ac.uk/pdbsum/6xls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xls ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.797Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=F2Y:3,5-DIFLUORO-L-TYROSINE'>F2Y</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xls OCA], [https://pdbe.org/6xls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xls RCSB], [https://www.ebi.ac.uk/pdbsum/6xls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xls ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GAOA_GIBZA GAOA_GIBZA] Catalyzes the sterospecific oxidation of primary alcohols to the corresponding aldehydes. The biologically relevant substrate of the enzyme is not known as the enzyme exhibits broad substrate specificity from small alcohols through sugars to oligo- and polysaccharides.<ref>PMID:13641238</ref> <ref>PMID:4441089</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Galactose oxidase (GAO) contains a Cu(II)-ligand radical cofactor. The cofactor, which is autocatalytically generated through the oxidation of the copper, consists of a cysteine-tyrosine radical (Cys-Tyr()) as a copper ligand. The formation of the cross-linked thioether bond is accompanied by a C-H bond scission on Tyr272 with few details known thus far. Here, we report the genetic incorporation of 3,5-dichlorotyrosine (Cl2-Tyr) and 3,5-difluorotyrosine (F2-Tyr) to replace Tyr272 in the GAO(V) previously optimized for expression through directed evolution. The proteins with an unnatural tyrosine residue are catalytically competent. We determined the high-resolution crystal structures of the GAO(V), Cl2-Tyr272, and F2-Tyr272 incorporated variants at 1.48, 1.23, and 1.80 A resolution, respectively. The structural data showed only one halogen remained in the cofactor, indicating that an oxidative carbon-chlorine/fluorine bond scission has occurred during the autocatalytic process of cofactor biogenesis. Using hydroxyurea as a radical scavenger, the spin-coupled hidden Cu(II) was observed by EPR spectroscopy. Thus, the structurally defined catalytic center with genetic unnatural tyrosine substitution is in the radical containing form as in the wild-type, i.e., Cu(II)-(Cl-Tyr()-Cys) or Cu(II)-(F-Tyr(*)-Cys). These findings illustrate a previously unobserved C-F/C-Cl bond cleavage in biology mediated by a mononuclear copper center.
		+
		+	Formation of Monofluorinated Radical Cofactor in Galactose Oxidase through Copper-Mediated C-F Bond Scission.,Li J, Davis I, Griffith WP, Liu A J Am Chem Soc. 2020 Nov 4;142(44):18753-18757. doi: 10.1021/jacs.0c08992. Epub, 2020 Oct 22. PMID:33091303<ref>PMID:33091303</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xls" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galactose oxidase\|Galactose oxidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium graminearum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Li J]]		[[Category: Li J]]
	[[Category: Liu A]]		[[Category: Liu A]]

OCA at 11:07, 3 February 2021

OCA — Wed, 03 Feb 2021 11:07:53 GMT

←Older revision		Revision as of 11:07, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry 6xls is ON HOLD until Paper Publication~~	+	==The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272==
-		+	<StructureSection load='6xls' size='340' side='right'caption='[[6xls]]' scene=''>
-	~~Authors: Liu, A., Li, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XLS FirstGlance]. <br>
-	~~Description:~~ The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xls OCA], [https://pdbe.org/6xls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xls RCSB], [https://www.ebi.ac.uk/pdbsum/6xls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xls ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Li, J]]	+	__TOC__
-	[[Category: Liu, A]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li J]]
		+	[[Category: Liu A]]

OCA at 09:23, 29 July 2020

OCA — Wed, 29 Jul 2020 09:23:47 GMT

←Older revision		Revision as of 09:23, 29 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xls is ON HOLD	+	The entry 6xls is ON HOLD until Paper Publication

	Authors: Liu, A., Li, J.		Authors: Liu, A., Li, J.

OCA: Protected "6xls" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 10:57:34 GMT

Protected "6xls" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:57, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xls is ON HOLD Authors: Liu, A., Li, J. Description: The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporate...

OCA — Wed, 08 Jul 2020 10:57:33 GMT

New page: '''Unreleased structure''' The entry 6xls is ON HOLD Authors: Liu, A., Li, J. Description: The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporate...

New page

'''Unreleased structure'''

The entry 6xls is ON HOLD

Authors: Liu, A., Li, J.

Description: The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272
[[Category: Unreleased Structures]]
[[Category: Li, J]]
[[Category: Liu, A]]