OCA at 14:58, 18 October 2023

OCA — Wed, 18 Oct 2023 14:58:50 GMT

←Older revision		Revision as of 14:58, 18 October 2023
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-	====	+	==Human antibody S8V2-37 in complex with the influenza hemagglutinin head domain of A/Texas/50/2012(H3N2)==
-	<StructureSection load='6xq2' size='340' side='right'caption='[[6xq2]]' scene=''>	+	<StructureSection load='6xq2' size='340' side='right'caption='[[6xq2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xq2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Texas/50/2012(H3N2)) Influenza A virus (A/Texas/50/2012(H3N2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XQ2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xq2 OCA], [https://pdbe.org/6xq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xq2 RCSB], [https://www.ebi.ac.uk/pdbsum/6xq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xq2 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.002Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xq2 OCA], [https://pdbe.org/6xq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xq2 RCSB], [https://www.ebi.ac.uk/pdbsum/6xq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xq2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R4L1D1_9INFA R4L1D1_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS00842802] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Novel animal influenza viruses emerge, initiate pandemics, and become endemic seasonal variants that have evolved to escape from prevalent herd immunity. These processes often outpace vaccine-elicited protection. Focusing immune responses on conserved epitopes may impart durable immunity. We describe a focused, protective antibody response, abundant in memory and serum repertoires, to a conserved region at the influenza virus hemagglutinin (HA) head interface. Structures of 11 examples, 8 reported here, from seven human donors demonstrate the convergence of responses on a single epitope. The 11 are genetically diverse, with one class having a common, IGkappaV1-39, light chain. All of the antibodies bind HAs from multiple serotypes. The lack of apparent genetic restriction and potential for elicitation by more than one serotype may explain their abundance. We define the head interface as a major target of broadly protective antibodies with the potential to influence the outcomes of influenza virus infection. IMPORTANCE The rapid appearance of mutations in circulating human influenza viruses and selection for escape from herd immunity require prediction of likely variants for an annual updating of influenza vaccines. The identification of human antibodies that recognize conserved surfaces on the influenza virus hemagglutinin (HA) has prompted efforts to design immunogens that might selectively elicit such antibodies. The recent discovery of a widely prevalent antibody response to the conserved interface between two HA "heads" (the globular, receptor-binding domains at the apex of the spike-like trimer) has added a new target for these efforts. We report structures of eight such antibodies, bound with HA heads, and compare them with each other and with three others previously described. Although genetically diverse, they all converge on a common binding site. The analysis here can guide immunogen design for preclinical trials.
		+
		+	A Prevalent Focused Human Antibody Response to the Influenza Virus Hemagglutinin Head Interface.,McCarthy KR, Lee J, Watanabe A, Kuraoka M, Robinson-McCarthy LR, Georgiou G, Kelsoe G, Harrison SC mBio. 2021 Jun 29;12(3):e0114421. doi: 10.1128/mBio.01144-21. Epub 2021 Jun 1. PMID:34060327<ref>PMID:34060327</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xq2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Harrison SC]]
		+	[[Category: McCarthy KR]]

OCA at 10:06, 19 May 2021

OCA — Wed, 19 May 2021 10:06:34 GMT

←Older revision		Revision as of 10:06, 19 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6xq2 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xq2' size='340' side='right'caption='[[6xq2]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xq2 OCA], [https://pdbe.org/6xq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xq2 RCSB], [https://www.ebi.ac.uk/pdbsum/6xq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xq2 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:06, 26 August 2020

OCA — Wed, 26 Aug 2020 09:06:05 GMT

←Older revision		Revision as of 09:06, 26 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xq2 is ON HOLD	+	The entry 6xq2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xq2" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 06:52:24 GMT

Protected "6xq2" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xq2 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jul 2020 06:52:23 GMT

New page: '''Unreleased structure''' The entry 6xq2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xq2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xq2 - Revision history

OCA at 14:58, 18 October 2023

OCA at 10:06, 19 May 2021

OCA at 09:06, 26 August 2020

OCA: Protected "6xq2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xq2 is ON HOLD Authors: Description: Category: Unreleased Structures