OCA at 12:03, 1 February 2023

OCA — Wed, 01 Feb 2023 12:03:28 GMT

←Older revision		Revision as of 12:03, 1 February 2023
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-	====	+	==Cryo-EM Structure of Vaccine-Elicited Rhesus Antibody 789-203-3C12 in Complex with Stabilized SI06 (A/Solomon Islands/3/06) Influenza Hemagglutinin Trimer==
-	<StructureSection load='6xsk' size='340' side='right'caption='[[6xsk]]' scene=''>	+	<StructureSection load='6xsk' size='340' side='right'caption='[[6xsk]], [[Resolution\|resolution]] 3.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xsk]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Solomon_Islands/3/2006(H1N1)) Influenza A virus (A/Solomon Islands/3/2006(H1N1))] and [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XSK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xsk OCA], [https://pdbe.org/6xsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xsk RCSB], [https://www.ebi.ac.uk/pdbsum/6xsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xsk ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xsk OCA], [https://pdbe.org/6xsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xsk RCSB], [https://www.ebi.ac.uk/pdbsum/6xsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xsk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A7Y8I1_9INFA A7Y8I1_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS01039073] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Current influenza vaccines predominantly induce immunity to the hypervariable hemagglutinin (HA) head, requiring frequent vaccine reformulation. Conversely, the immunosubdominant yet conserved HA stem harbors a supersite that is targeted by broadly neutralizing antibodies (bnAbs), representing a prime target for universal vaccines. Here, we showed that the co-immunization of two HA stem immunogens derived from group 1 and 2 influenza A viruses elicits cross-group protective immunity and neutralizing antibody responses in mice, ferrets, and nonhuman primates (NHPs). Immunized mice were protected from multiple group 1 and 2 viruses, and all animal models showed broad serum-neutralizing activity. A bnAb isolated from an immunized NHP broadly neutralized and protected against diverse viruses, including H5N1 and H7N9. Genetic and structural analyses revealed strong homology between macaque and human bnAbs, illustrating common biophysical constraints for acquiring cross-group specificity. Vaccine elicitation of stem-directed cross-group-protective immunity represents a step toward the development of broadly protective influenza vaccines.
		+
		+	Co-immunization with hemagglutinin stem immunogens elicits cross-group neutralizing antibodies and broad protection against influenza A viruses.,Moin SM, Boyington JC, Boyoglu-Barnum S, Gillespie RA, Cerutti G, Cheung CS, Cagigi A, Gallagher JR, Brand J, Prabhakaran M, Tsybovsky Y, Stephens T, Fisher BE, Creanga A, Ataca S, Rawi R, Corbett KS, Crank MC, Karlsson Hedestam GB, Gorman J, McDermott AB, Harris AK, Zhou T, Kwong PD, Shapiro L, Mascola JR, Graham BS, Kanekiyo M Immunity. 2022 Dec 13;55(12):2405-2418.e7. doi: 10.1016/j.immuni.2022.10.015. , Epub 2022 Nov 9. PMID:36356572<ref>PMID:36356572</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Macaca mulatta]]
		+	[[Category: Cerutti G]]
		+	[[Category: Gorman J]]
		+	[[Category: Kwong PD]]
		+	[[Category: Shapiro L]]

OCA at 08:55, 21 July 2021

OCA — Wed, 21 Jul 2021 08:55:37 GMT

←Older revision		Revision as of 08:55, 21 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6xsk ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xsk' size='340' side='right'caption='[[6xsk]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xsk OCA], [https://pdbe.org/6xsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xsk RCSB], [https://www.ebi.ac.uk/pdbsum/6xsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xsk ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:53, 5 August 2020

OCA — Wed, 05 Aug 2020 03:53:16 GMT

←Older revision		Revision as of 03:53, 5 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xsk is ON HOLD	+	The entry 6xsk is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xsk" [edit=sysop:move=sysop]

OCA — Wed, 22 Jul 2020 07:01:20 GMT

Protected "6xsk" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:01, 22 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xsk is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jul 2020 07:01:19 GMT

New page: '''Unreleased structure''' The entry 6xsk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xsk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xsk - Revision history

OCA at 12:03, 1 February 2023

OCA at 08:55, 21 July 2021

OCA at 03:53, 5 August 2020

OCA: Protected "6xsk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xsk is ON HOLD Authors: Description: Category: Unreleased Structures