6xwm - Revision history

OCA at 13:14, 24 January 2024

OCA — Wed, 24 Jan 2024 13:14:17 GMT

←Older revision		Revision as of 13:14, 24 January 2024
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	<StructureSection load='6xwm' size='340' side='right'caption='[[6xwm]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='6xwm' size='340' side='right'caption='[[6xwm]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6xwm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aquae Aquae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XWM FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~, ~~<scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~snf, aq_2077 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=224324 AQUAE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwm OCA], [~~http~~://pdbe.org/6xwm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xwm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xwm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwm OCA], [https://pdbe.org/6xwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xwm RCSB], [https://www.ebi.ac.uk/pdbsum/6xwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
		+	*[[Leucine transporter\|Leucine transporter]]
	*[[Symporter 3D structures\|Symporter 3D structures]]		*[[Symporter 3D structures\|Symporter 3D structures]]
	== References ==		== References ==
Line 24:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aquae~~]]	+	[[Category: Aquifex aeolicus VF5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boesen, T]]	+	[[Category: Boesen T]]
-	[[Category: Gether, U]]	+	[[Category: Gether U]]
-	[[Category: Gotfryd, K]]	+	[[Category: Gotfryd K]]
-	[[Category: Loland~~, C J~~]]	+	[[Category: Loland CJ]]
-	[[Category: Nissen, P]]	+	[[Category: Nissen P]]
-	~~[[Category: Membrane protein~~]]	+

OCA at 07:12, 25 November 2020

OCA — Wed, 25 Nov 2020 07:12:53 GMT

←Older revision		Revision as of 07:12, 25 November 2020
Line 1:		Line 1:

	==Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of LeuT in an inward-facing occluded conformation==		==Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of LeuT in an inward-facing occluded conformation==
-	<StructureSection load='6xwm' size='340' side='right'caption='[[6xwm]]' scene=''>	+	<StructureSection load='6xwm' size='340' side='right'caption='[[6xwm]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquae Aquae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XWM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwm OCA], [http://pdbe.org/6xwm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwm RCSB], [http://www.ebi.ac.uk/pdbsum/6xwm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwm ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snf, aq_2077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224324 AQUAE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwm OCA], [http://pdbe.org/6xwm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwm RCSB], [http://www.ebi.ac.uk/pdbsum/6xwm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwm ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neurotransmitter:sodium symporters (NSS) are conserved from bacteria to man and serve as targets for drugs, including antidepressants and psychostimulants. Here we report the X-ray structure of the prokaryotic NSS member, LeuT, in a Na(+)/substrate-bound, inward-facing occluded conformation. To obtain this structure, we were guided by findings from single-molecule fluorescence spectroscopy and molecular dynamics simulations indicating that L-Phe binding and mutation of the conserved N-terminal Trp8 to Ala both promote an inward-facing state. Compared to the outward-facing occluded conformation, our structure reveals a major tilting of the cytoplasmic end of transmembrane segment (TM) 5, which, together with release of the N-terminus but without coupled movement of TM1, opens a wide cavity towards the second Na(+) binding site. The structure of this key intermediate in the LeuT transport cycle, in the context of other NSS structures, leads to the proposal of an intracellular release mechanism of substrate and ions in NSS proteins.
		+
		+	X-ray structure of LeuT in an inward-facing occluded conformation reveals mechanism of substrate release.,Gotfryd K, Boesen T, Mortensen JS, Khelashvili G, Quick M, Terry DS, Missel JW, LeVine MV, Gourdon P, Blanchard SC, Javitch JA, Weinstein H, Loland CJ, Nissen P, Gether U Nat Commun. 2020 Feb 21;11(1):1005. doi: 10.1038/s41467-020-14735-w. PMID:32081981<ref>PMID:32081981</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xwm" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Symporter 3D structures\|Symporter 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aquae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boesen T]]	+	[[Category: Boesen, T]]
		+	[[Category: Gether, U]]
		+	[[Category: Gotfryd, K]]
		+	[[Category: Loland, C J]]
		+	[[Category: Nissen, P]]
		+	[[Category: Membrane protein]]

OCA at 06:08, 6 May 2020

OCA — Wed, 06 May 2020 06:08:30 GMT

←Older revision		Revision as of 06:08, 6 May 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xwm ~~is ON HOLD~~	+	==Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of LeuT in an inward-facing occluded conformation==
-		+	<StructureSection load='6xwm' size='340' side='right'caption='[[6xwm]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XWM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwm OCA], [http://pdbe.org/6xwm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwm RCSB], [http://www.ebi.ac.uk/pdbsum/6xwm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwm ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Boesen T]]

OCA: Protected "6xwm" [edit=sysop:move=sysop]

OCA — Wed, 29 Jan 2020 13:53:03 GMT

Protected "6xwm" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:53, 29 January 2020

OCA: New page: '''Unreleased structure''' The entry 6xwm is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Jan 2020 13:53:01 GMT

New page: '''Unreleased structure''' The entry 6xwm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xwm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]