6xws - Revision history

OCA at 13:14, 24 January 2024

OCA — Wed, 24 Jan 2024 13:14:18 GMT

←Older revision		Revision as of 13:14, 24 January 2024
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	==Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A/H4==		==Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A/H4==
-	<StructureSection load='6xws' size='340' side='right'caption='[[6xws]]' scene=''>	+	<StructureSection load='6xws' size='340' side='right'caption='[[6xws]], [[Resolution\|resolution]] 4.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xws]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XWS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xws OCA], [~~http~~://pdbe.org/6xws PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xws RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xws PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xws ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.36Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xws OCA], [https://pdbe.org/6xws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xws RCSB], [https://www.ebi.ac.uk/pdbsum/6xws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xws ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CID_DROME CID_DROME] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:11483958, PubMed:16839185). Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (PubMed:24703848, PubMed:11483958, PubMed:16839185). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:11483958, PubMed:16839185).<ref>PMID:11483958</ref> <ref>PMID:16839185</ref> <ref>PMID:24703848</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Centromeres are microtubule attachment sites on chromosomes defined by the enrichment of histone variant CENP-A-containing nucleosomes. To preserve centromere identity, CENP-A must be escorted to centromeres by a CENP-A-specific chaperone for deposition. Despite this essential requirement, many eukaryotes differ in the composition of players involved in centromere maintenance, highlighting the plasticity of this process. In humans, CENP-A recognition and centromere targeting are achieved by HJURP and the Mis18 complex, respectively. Using X-ray crystallography, we here show how Drosophila CAL1, an evolutionarily distinct CENP-A histone chaperone, binds both CENP-A and the centromere receptor CENP-C without the requirement for the Mis18 complex. While an N-terminal CAL1 fragment wraps around CENP-A/H4 through multiple physical contacts, a C-terminal CAL1 fragment directly binds a CENP-C cupin domain dimer. Although divergent at the primary structure level, CAL1 thus binds CENP-A/H4 using evolutionarily conserved and adaptive structural principles. The CAL1 binding site on CENP-C is strategically positioned near the cupin dimerisation interface, restricting binding to just one CAL1 molecule per CENP-C dimer. Overall, by demonstrating how CAL1 binds CENP-A/H4 and CENP-C, we provide key insights into the minimalistic principles underlying centromere maintenance.
		+
		+	Structural basis for centromere maintenance by Drosophila CENP-A chaperone CAL1.,Medina-Pritchard B, Lazou V, Zou J, Byron O, Abad MA, Rappsilber J, Heun P, Jeyaprakash AA EMBO J. 2020 Mar 5:e103234. doi: 10.15252/embj.2019103234. PMID:32134144<ref>PMID:32134144</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xws" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histone 3D structures\|Histone 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abad MA]]		[[Category: Abad MA]]

OCA at 07:04, 15 April 2020

OCA — Wed, 15 Apr 2020 07:04:55 GMT

←Older revision		Revision as of 07:04, 15 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xws is ~~ON HOLD until sometime in~~ the ~~future~~	+	==Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A/H4==
-		+	<StructureSection load='6xws' size='340' side='right'caption='[[6xws]]' scene=''>
-	~~Authors~~: ~~Jeyaprakash, A~~.A., ~~Medina~~-~~Pritchard~~, B., ~~Lazou, V~~.~~, Zou, J~~.~~, Byron, O~~., ~~Abad, M~~.A.~~, Rappsilber, J~~., ~~Heun, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XWS FirstGlance]. <br>
-	~~Description: Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A~~/H4	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xws OCA], [http://pdbe.org/6xws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xws RCSB], [http://www.ebi.ac.uk/pdbsum/6xws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xws ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Rappsilber, J~~]]	+	__TOC__
-	[[Category: ~~Medina-Pritchard, B~~]]	+	</StructureSection>
-	[[Category: ~~Zou, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Byron, O~~]]	+	[[Category: Abad MA]]
-	[[Category: ~~Jeyaprakash, A.A~~]]	+	[[Category: Byron O]]
-	[[Category: ~~Heun, P~~]]	+	[[Category: Heun P]]
-	[[Category: ~~Lazou, V~~]]	+	[[Category: Jeyaprakash AA]]
-	[[Category: ~~Abad, M.A~~]]	+	[[Category: Lazou V]]
		+	[[Category: Medina-Pritchard B]]
		+	[[Category: Rappsilber J]]
		+	[[Category: Zou J]]

OCA: Protected "6xws" [edit=sysop:move=sysop]

OCA — Wed, 05 Feb 2020 06:26:08 GMT

Protected "6xws" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:26, 5 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xws is ON HOLD until sometime in the future Authors: Jeyaprakash, A.A., Medina-Pritchard, B., Lazou, V., Zou, J., Byron, O., Abad, M.A., Rappsilber...

OCA — Wed, 05 Feb 2020 06:26:06 GMT

New page: '''Unreleased structure''' The entry 6xws is ON HOLD until sometime in the future Authors: Jeyaprakash, A.A., Medina-Pritchard, B., Lazou, V., Zou, J., Byron, O., Abad, M.A., Rappsilber...

New page

'''Unreleased structure'''

The entry 6xws is ON HOLD until sometime in the future

Authors: Jeyaprakash, A.A., Medina-Pritchard, B., Lazou, V., Zou, J., Byron, O., Abad, M.A., Rappsilber, J., Heun, P.

Description: Crystal Structure of Drosophila CAL1 1-160 bound to CENP-A/H4
[[Category: Unreleased Structures]]
[[Category: Rappsilber, J]]
[[Category: Medina-Pritchard, B]]
[[Category: Zou, J]]
[[Category: Byron, O]]
[[Category: Jeyaprakash, A.A]]
[[Category: Heun, P]]
[[Category: Lazou, V]]
[[Category: Abad, M.A]]