OCA at 13:14, 24 January 2024

OCA — Wed, 24 Jan 2024 13:14:42 GMT

←Older revision		Revision as of 13:14, 24 January 2024
Line 1:		Line 1:

	==Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II==		==Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II==
-	<StructureSection load='6xx8' size='340' side='right'caption='[[6xx8]]' scene=''>	+	<StructureSection load='6xx8' size='340' side='right'caption='[[6xx8]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XX8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xx8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XX8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xx8 OCA], [~~http~~://pdbe.org/6xx8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xx8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xx8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xx8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xx8 OCA], [https://pdbe.org/6xx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xx8 RCSB], [https://www.ebi.ac.uk/pdbsum/6xx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xx8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CSK21_ARATH CSK21_ARATH] Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates casein in vitro (PubMed:7678767). The alpha chain contains the catalytic site. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor GBFl, resulting in stimulation of its DNA binding activity (PubMed:7696877). CK2 phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis (PubMed:21330376). CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation (PubMed:19509278, PubMed:19509420). Acts as circadian clock component that maintains the correct period length through phosphorylation of CCA1 (PubMed:21900482). Required for the maintenance and control of genomic stability and chromatin structure (PubMed:22487192). May act as an ectokinase that phosphorylates several extracellular proteins.<ref>PMID:19509278</ref> <ref>PMID:19509420</ref> <ref>PMID:21330376</ref> <ref>PMID:21900482</ref> <ref>PMID:22487192</ref> <ref>PMID:7678767</ref> <ref>PMID:7696877</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Casein kinase 2 (CK2) is a ubiquitous pleiotropic enzyme that is highly conserved across eukaryotic kingdoms. CK2 is singular amongst kinases as it is highly rigid and constitutively active. Arabidopsis thaliana is widely used as a model system in molecular plant research; the biological functions of A. thaliana CK2 are well studied in vivo and many of its substrates have been identified. Here, crystal structures of the alpha subunit of A. thaliana CK2 in three crystal forms and of its complex with the nonhydrolyzable ATP analog AMppNHp are presented. While the C-lobe of the enzyme is highly rigid, structural plasticity is observed for the N-lobe. Small but significant displacements within the active cleft are necessary in order to avoid steric clashes with the AMppNHp molecule. Binding of AMppNHp is influenced by a rigid-body motion of the N-lobe that was not previously recognized in maize CK2.
		+
		+	Crystal structure of Arabidopsis thaliana casein kinase 2 alpha1.,Demulder M, De Veylder L, Loris R Acta Crystallogr F Struct Biol Commun. 2020 Apr 1;76(Pt 4):182-191. doi:, 10.1107/S2053230X20004537. Epub 2020 Apr 6. PMID:32254052<ref>PMID:32254052</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xx8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Casein kinase 3D structures\|Casein kinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: De Veylder L]]		[[Category: De Veylder L]]
	[[Category: Demulder M]]		[[Category: Demulder M]]
	[[Category: Loris R]]		[[Category: Loris R]]

OCA at 07:05, 15 April 2020

OCA — Wed, 15 Apr 2020 07:05:15 GMT

←Older revision		Revision as of 07:05, 15 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6xx8 is ON HOLD until Paper Publication~~	+	==Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II==
-		+	<StructureSection load='6xx8' size='340' side='right'caption='[[6xx8]]' scene=''>
-	~~Authors: Demulder, M., De Veylder, L., Loris, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XX8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XX8 FirstGlance]. <br>
-	~~Description:~~ Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xx8 OCA], [http://pdbe.org/6xx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xx8 RCSB], [http://www.ebi.ac.uk/pdbsum/6xx8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xx8 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Loris~~, R]]	+	__TOC__
-	[[Category: De Veylder, L]]	+	</StructureSection>
-	[[Category: Demulder, M]]	+	[[Category: Large Structures]]
		+	[[Category: De Veylder L]]
		+	[[Category: Demulder M]]
		+	[[Category: Loris R]]

OCA: Protected "6xx8" [edit=sysop:move=sysop]

OCA — Wed, 05 Feb 2020 06:27:33 GMT

Protected "6xx8" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 5 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xx8 is ON HOLD until Paper Publication Authors: Demulder, M., De Veylder, L., Loris, R. Description: Arabidopsis thaliana Casein Kinase 2 (CK2) al...

OCA — Wed, 05 Feb 2020 06:27:31 GMT

New page: '''Unreleased structure''' The entry 6xx8 is ON HOLD until Paper Publication Authors: Demulder, M., De Veylder, L., Loris, R. Description: Arabidopsis thaliana Casein Kinase 2 (CK2) al...

New page

'''Unreleased structure'''

The entry 6xx8 is ON HOLD until Paper Publication

Authors: Demulder, M., De Veylder, L., Loris, R.

Description: Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II
[[Category: Unreleased Structures]]
[[Category: Loris, R]]
[[Category: De Veylder, L]]
[[Category: Demulder, M]]

6xx8 - Revision history

OCA at 13:14, 24 January 2024

OCA at 07:05, 15 April 2020

OCA: Protected "6xx8" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xx8 is ON HOLD until Paper Publication Authors: Demulder, M., De Veylder, L., Loris, R. Description: Arabidopsis thaliana Casein Kinase 2 (CK2) al...