OCA at 13:14, 24 January 2024

OCA — Wed, 24 Jan 2024 13:14:47 GMT

←Older revision		Revision as of 13:14, 24 January 2024
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-	====	+	==1.7 Angstrom crystal structure of Ca/CaM:RyR2 peptide complex==
-	<StructureSection load='6xxf' size='340' side='right'caption='[[6xxf]]' scene=''>	+	<StructureSection load='6xxf' size='340' side='right'caption='[[6xxf]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xxf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XXF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xxf OCA], [https://pdbe.org/6xxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xxf RCSB], [https://www.ebi.ac.uk/pdbsum/6xxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xxf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xxf OCA], [https://pdbe.org/6xxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xxf RCSB], [https://www.ebi.ac.uk/pdbsum/6xxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xxf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CALM2_HUMAN CALM2_HUMAN] Catecholaminergic polymorphic ventricular tachycardia;Brugada syndrome;Romano-Ward syndrome. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM2 are the cause of LQT15.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CALM2_HUMAN CALM2_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an inherited condition that can cause fatal cardiac arrhythmia. Human mutations in the Ca2+ sensor calmodulin (CaM) have been associated with CPVT susceptibility, suggesting that CaM dysfunction is a key driver of the disease. However, the detailed molecular mechanism remains unclear. Focusing on the interaction with the cardiac ryanodine receptor (RyR2), we determined the effect of CPVT-associated variants N53I and A102V on the structural characteristics of CaM and on Ca2+ fluxes in live cells. We provide novel data showing that interaction of both Ca2+/CaM-N53I and Ca2+/CaM-A102V with the RyR2 binding domain is decreased. Ca2+/CaM-RyR23583-3603 high-resolution crystal structures highlight subtle conformational changes for the N53I variant, with A102V being similar to wild type (WT). We show that co-expression of CaM-N53I or CaM-A102V with RyR2 in HEK293 cells significantly increased the duration of Ca2+ events; CaM-A102V exhibited a lower frequency of Ca2+ oscillations. In addition, we show that CaMKIIdelta (also known as CAMK2D) phosphorylation activity is increased for A102V, compared to CaM-WT. This paper provides novel insight into the molecular mechanisms of CPVT-associated CaM variants and will facilitate the development of strategies for future therapies.
		+
		+	CPVT-associated calmodulin variants N53I and A102V dysregulate Ca2+ signalling via different mechanisms.,Prakash O, Held M, McCormick LF, Gupta N, Lian LY, Antonyuk S, Haynes LP, Thomas NL, Helassa N J Cell Sci. 2022 Jan 15;135(2):jcs258796. doi: 10.1242/jcs.258796. Epub 2022 Jan , 18. PMID:34888671<ref>PMID:34888671</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xxf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Antonyuk S]]
		+	[[Category: Helassa N]]

OCA at 10:55, 10 February 2021

OCA — Wed, 10 Feb 2021 10:55:18 GMT

←Older revision		Revision as of 10:55, 10 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6xxf ~~is ON HOLD until Jan 27 2022~~	+	====
-		+	<StructureSection load='6xxf' size='340' side='right'caption='[[6xxf]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xxf OCA], [https://pdbe.org/6xxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xxf RCSB], [https://www.ebi.ac.uk/pdbsum/6xxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xxf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:05, 22 July 2020

OCA — Wed, 22 Jul 2020 07:05:46 GMT

←Older revision		Revision as of 07:05, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xxf is ON HOLD	+	The entry 6xxf is ON HOLD until Jan 27 2022

	Authors:		Authors:

OCA: Protected "6xxf" [edit=sysop:move=sysop]

OCA — Wed, 05 Feb 2020 06:27:58 GMT

Protected "6xxf" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 5 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xxf is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Feb 2020 06:27:56 GMT

New page: '''Unreleased structure''' The entry 6xxf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xxf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xxf - Revision history

OCA at 13:14, 24 January 2024

OCA at 10:55, 10 February 2021

OCA at 07:05, 22 July 2020

OCA: Protected "6xxf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xxf is ON HOLD Authors: Description: Category: Unreleased Structures