OCA at 10:04, 18 March 2020

OCA — Wed, 18 Mar 2020 10:04:00 GMT

←Older revision		Revision as of 10:04, 18 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xy0 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a de novo designed parallel four-helix coiled coil, 3-EK-4==
		+	<StructureSection load='6xy0' size='340' side='right'caption='[[6xy0]], [[Resolution\|resolution]] 1.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6xy0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XY0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6XY0 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6xy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xy0 OCA], [http://pdbe.org/6xy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6xy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xy0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	De novo-designed protein domains are increasingly being applied in biotechnology, cell biology, and synthetic biology. Therefore, it is imperative that these proteins be robust to superficial changes; i.e., small changes to their amino acid sequences should not cause gross structural changes. In turn, this allows properties such as stability and solubility to be tuned without affecting structural attributes like tertiary fold and quaternary interactions. Reliably designed proteins with predictable behaviors may then be used as scaffolds to incorporate function, e.g., through the introduction of features for small-molecule, metal, or macromolecular binding, and enzyme-like active sites. Generally, achieving this requires the starting protein fold to be well understood. Herein, we focus on designing alpha-helical coiled coils, which are well studied, widespread, and often direct protein-protein interactions in natural systems. Our initial investigations reveal that a previously designed parallel, homotetrameric coiled coil, CC-Tet, is not robust to sequence changes that were anticipated to maintain its structure. Instead, the alterations switch the oligomeric state from tetramer to trimer. To improve the robustness of designed homotetramers, additional sequences based on CC-Tet were produced and characterized in solution and by X-ray crystallography. Of these updated sequences, one is robust to truncation and to changes in surface electrostatics; we call this CC-Tet. Variants of the general CC-Tet design provide a set of homotetrameric coiled coils with unfolding temperatures in the range from 40 to >95 degrees C. We anticipate that these will be of use in applications requiring robust and well-defined tetramerization domains.

-	~~Authors: Edgell, C.L~~., Savery, N.J.~~, Woolfson, D~~.N.	+	Robust De Novo-Designed Homotetrameric Coiled Coils.,Edgell CL, Savery NJ, Woolfson DN Biochemistry. 2020 Mar 17;59(10):1087-1092. doi: 10.1021/acs.biochem.0c00082., Epub 2020 Mar 9. PMID:32133841<ref>PMID:32133841</ref>

-	~~Description: Crystal structure of~~ a ~~de novo designed parallel four~~-~~helix coiled coil, 3~~-~~EK-4~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Savery~~, ~~N.J~~]]	+	<div class="pdbe-citations 6xy0" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Edgell~~, ~~C.L~~]]	+	== References ==
-	[[Category: Woolfson, D.N]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Edgell, C L]]
		+	[[Category: Savery, N J]]
		+	[[Category: Woolfson, D N]]
		+	[[Category: Coiled coil]]
		+	[[Category: De novo protein]]
		+	[[Category: Parallel]]
		+	[[Category: Tetramer]]

OCA: Protected "6xy0" [edit=sysop:move=sysop]

OCA — Wed, 05 Feb 2020 06:29:30 GMT

Protected "6xy0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:29, 5 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xy0 is ON HOLD until Paper Publication Authors: Edgell, C.L., Savery, N.J., Woolfson, D.N. Description: Crystal structure of a de novo designed pa...

OCA — Wed, 05 Feb 2020 06:29:28 GMT

New page: '''Unreleased structure''' The entry 6xy0 is ON HOLD until Paper Publication Authors: Edgell, C.L., Savery, N.J., Woolfson, D.N. Description: Crystal structure of a de novo designed pa...

New page

'''Unreleased structure'''

The entry 6xy0 is ON HOLD until Paper Publication

Authors: Edgell, C.L., Savery, N.J., Woolfson, D.N.

Description: Crystal structure of a de novo designed parallel four-helix coiled coil, 3-EK-4
[[Category: Unreleased Structures]]
[[Category: Savery, N.J]]
[[Category: Edgell, C.L]]
[[Category: Woolfson, D.N]]

6xy0 - Revision history

OCA at 10:04, 18 March 2020

OCA: Protected "6xy0" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xy0 is ON HOLD until Paper Publication Authors: Edgell, C.L., Savery, N.J., Woolfson, D.N. Description: Crystal structure of a de novo designed pa...