OCA at 12:45, 13 October 2021

OCA — Wed, 13 Oct 2021 12:45:44 GMT

←Older revision		Revision as of 12:45, 13 October 2021
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	==Structure of the T4Lnano fusion protein==		==Structure of the T4Lnano fusion protein==
-	<StructureSection load='6xyr' size='340' side='right'caption='[[6xyr]]' scene=''>	+	<StructureSection load='6xyr' size='340' side='right'caption='[[6xyr]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XYR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xyr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XYR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyr OCA], [~~http~~://pdbe.org/6xyr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xyr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xyr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6hr1\|6hr1]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CALM1, CALM, CAM, CAM1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyr OCA], [https://pdbe.org/6xyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xyr RCSB], [https://www.ebi.ac.uk/pdbsum/6xyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyr ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chimeric fusion proteins are essential tools for protein nanotechnology. Non-optimized protein-protein connections are usually flexible and therefore unsuitable as structural building blocks. Here we show that the ER/K motif, a single alpha-helical domain (SAH), can be seamlessly fused to terminal helices of proteins, forming an extended, partially free-standing rigid helix. This enables the connection of two domains at a defined distance and orientation. We designed three constructs termed YFPnano, T4Lnano, and MoStoNano. Analysis of experimentally determined structures and molecular dynamics simulations reveals a certain degree of plasticity in the connections that allows the adaptation to crystal contact opportunities. Our data show that SAHs can be stably integrated into designed structural elements, enabling new possibilities for protein nanotechnology, for example, to improve the exposure of epitopes on nanoparticles (structural vaccinology), to engineer crystal contacts with minimal impact on construct flexibility (for the study of protein dynamics), and to design novel biomaterials.
		+
		+	Chimeric single alpha-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology.,Collu G, Bierig T, Krebs AS, Engilberge S, Varma N, Guixa-Gonzalez R, Sharpe T, Deupi X, Olieric V, Poghosyan E, Benoit RM Structure. 2021 Sep 24. pii: S0969-2126(21)00330-0. doi:, 10.1016/j.str.2021.09.002. PMID:34587504<ref>PMID:34587504</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xyr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Benoit RM]]	+	[[Category: Benoit, R M]]
-	[[Category: Bierig T]]	+	[[Category: Bierig, T]]
-	[[Category: Collu C]]	+	[[Category: Collu, C]]
-	[[Category: Engilberge S]]	+	[[Category: Engilberge, S]]
-	[[Category: Olieric V]]	+	[[Category: Olieric, V]]
		+	[[Category: Crystal engineering]]
		+	[[Category: Fusion protein]]
		+	[[Category: Molecular biomimetic]]
		+	[[Category: Rigid helix]]
		+	[[Category: Structural protein]]

OCA at 06:37, 9 December 2020

OCA — Wed, 09 Dec 2020 06:37:28 GMT

←Older revision		Revision as of 06:37, 9 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xyr is ~~ON HOLD until Paper Publication~~	+	==Structure of the T4Lnano fusion protein==
-		+	<StructureSection load='6xyr' size='340' side='right'caption='[[6xyr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYR FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyr OCA], [http://pdbe.org/6xyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyr RCSB], [http://www.ebi.ac.uk/pdbsum/6xyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Benoit RM]]
		+	[[Category: Bierig T]]
		+	[[Category: Collu C]]
		+	[[Category: Engilberge S]]
		+	[[Category: Olieric V]]

OCA at 05:24, 19 February 2020

OCA — Wed, 19 Feb 2020 05:24:07 GMT

←Older revision		Revision as of 05:24, 19 February 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xyr is ON HOLD	+	The entry 6xyr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xyr" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2020 02:51:50 GMT

Protected "6xyr" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:51, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xyr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 13 Feb 2020 02:51:48 GMT

New page: '''Unreleased structure''' The entry 6xyr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xyr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xyr - Revision history

OCA at 12:45, 13 October 2021

OCA at 06:37, 9 December 2020

OCA at 05:24, 19 February 2020

OCA: Protected "6xyr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xyr is ON HOLD Authors: Description: Category: Unreleased Structures