OCA at 09:36, 12 May 2021

OCA — Wed, 12 May 2021 09:36:31 GMT

←Older revision		Revision as of 09:36, 12 May 2021
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	==NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==		==NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==
-	<StructureSection load='6xyv' size='340' side='right'caption='[[6xyv]]' scene=''>	+	<StructureSection load='6xyv' size='340' side='right'caption='[[6xyv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xyv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhopt Rhopt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XYV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyv OCA], [~~http~~://pdbe.org/6xyv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xyv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xyv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyv ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpal_0815 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395960 RHOPT])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyv OCA], [https://pdbe.org/6xyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xyv RCSB], [https://www.ebi.ac.uk/pdbsum/6xyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metalloproteins play key roles across biology, and knowledge of their structure is essential to understand their physiological role. For those metalloproteins containing paramagnetic states, the enhanced relaxation caused by the unpaired electrons often makes signal detection unfeasible near the metal center, precluding adequate structural characterization right where it is more biochemically relevant. Here, we report a protein structure determination by NMR where two different sets of restraints, one containing Nuclear Overhauser Enhancements (NOEs) and another containing Paramagnetic Relaxation Enhancements (PREs), are used separately and eventually together. The protein PioC from Rhodopseudomonas palustris TIE-1 is a High Potential Iron-Sulfur Protein (HiPIP) where the [4Fe-4S] cluster is paramagnetic in both oxidation states at room temperature providing the source of PREs used as alternative distance restraints. Comparison of the family of structures obtained using NOEs only, PREs only and the combination of both reveals that the pairwise root-mean-square deviation (RMSD) between them are similar and comparable with the precision within each family. This demonstrates that, under favorable conditions in terms of protein size and paramagnetic effects, PREs can efficiently complement and eventually replace NOEs for the structural characterization of small paramagnetic metalloproteins and denovo designed metalloproteins by NMR.
		+
		+	PRE-driven Protein NMR Structures: an Alternative Approach in Highly Paramagnetic Systems.,Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M FEBS J. 2020 Oct 30. doi: 10.1111/febs.15615. PMID:33124176<ref>PMID:33124176</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xyv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cantini F]]	+	[[Category: Rhopt]]
-	[[Category: Trindade I]]	+	[[Category: Cantini, F]]
		+	[[Category: Trindade, I]]
		+	[[Category: Electron transport]]
		+	[[Category: Iron-sulphur protein]]
		+	[[Category: Metallo protein]]
		+	[[Category: Paramagnetic nmr]]
		+	[[Category: Paramagnetic protein]]
		+	[[Category: Solution structure by nmr]]

OCA at 08:00, 11 November 2020

OCA — Wed, 11 Nov 2020 08:00:17 GMT

←Older revision		Revision as of 08:00, 11 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xyv ~~is ON HOLD until Paper Publication~~	+	==NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==
-		+	<StructureSection load='6xyv' size='340' side='right'caption='[[6xyv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyv OCA], [http://pdbe.org/6xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyv RCSB], [http://www.ebi.ac.uk/pdbsum/6xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cantini F]]
		+	[[Category: Trindade I]]

OCA at 04:12, 13 May 2020

OCA — Wed, 13 May 2020 04:12:49 GMT

←Older revision		Revision as of 04:12, 13 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xyv is ON HOLD	+	The entry 6xyv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xyv" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2020 02:52:06 GMT

Protected "6xyv" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:52, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xyv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 13 Feb 2020 02:52:04 GMT

New page: '''Unreleased structure''' The entry 6xyv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xyv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xyv - Revision history

OCA at 09:36, 12 May 2021

OCA at 08:00, 11 November 2020

OCA at 04:12, 13 May 2020

OCA: Protected "6xyv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xyv is ON HOLD Authors: Description: Category: Unreleased Structures