OCA at 06:18, 22 April 2020

OCA — Wed, 22 Apr 2020 06:18:36 GMT

←Older revision		Revision as of 06:18, 22 April 2020
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	==Structure of the plant mitochondrial ribosome==		==Structure of the plant mitochondrial ribosome==
-	<StructureSection load='6xyw' size='340' side='right'caption='[[6xyw]]' scene=''>	+	<StructureSection load='6xyw' size='340' side='right'caption='[[6xyw]], [[Resolution\|resolution]] 3.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xyw]] is a 89 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYW FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyw OCA], [http://pdbe.org/6xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyw RCSB], [http://www.ebi.ac.uk/pdbsum/6xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyw ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyw OCA], [http://pdbe.org/6xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyw RCSB], [http://www.ebi.ac.uk/pdbsum/6xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RM21_ARATH RM21_ARATH]] This protein binds to 23S ribosomal RNA in the presence of protein L20 (By similarity). Required for karyogamy during female gametophyte development, when the two polar nuclei fuse to form the diploid central cell nucleus, and during double fertilization of the egg cell and the central cell (PubMed:16698901).<ref>PMID:16698901</ref> [[http://www.uniprot.org/uniprot/HLP_ARATH HLP_ARATH]] Binds to 23S rRNA in mitochondrion. [[http://www.uniprot.org/uniprot/RT11_ARATH RT11_ARATH]] Required for karyogamy during female gametophyte development, when the two polar nuclei fuse to form the diploid central cell nucleus.<ref>PMID:16698901</ref> [[http://www.uniprot.org/uniprot/PP438_ARATH PP438_ARATH]] RNA-binding protein that functions in both mitochondrion and nucleus. In mitochondrion, it is associated with polysomes and may play a role in translation. Required during embryogenesis. In nucleus, might be involved in the regulation of its own gene expression.<ref>PMID:21297037</ref> [[http://www.uniprot.org/uniprot/Q8LCN1_ARATH Q8LCN1_ARATH]] Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.[RuleBase:RU004311] [[http://www.uniprot.org/uniprot/RT13_ARATH RT13_ARATH]] Located at the top of the head of the small subunit, it contacts several helices of the 18S rRNA. [[http://www.uniprot.org/uniprot/RK3B_ARATH RK3B_ARATH]] One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. [[http://www.uniprot.org/uniprot/RT19_ARATH RT19_ARATH]] The RNA-binding domain found in RPS19 may functionally replaces the missing mitochondrial RPS13. [[http://www.uniprot.org/uniprot/RPS9M_ARATH RPS9M_ARATH]] Mitochondrial ribosomal protein required for central cell maturation. May work together with PIA2 in controlling female gametophyte development, possibly by regulating the expression of some mitochondrial proteins.<ref>PMID:29312411</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The vast majority of eukaryotic cells contain mitochondria, essential powerhouses and metabolic hubs(1). These organelles have a bacterial origin and were acquired during an early endosymbiosis event(2). Mitochondria possess specialized gene expression systems composed of various molecular machines, including the mitochondrial ribosomes (mitoribosomes). Mitoribosomes are in charge of translating the few essential mRNAs still encoded by mitochondrial genomes(3). While chloroplast ribosomes strongly resemble those of bacteria(4,5), mitoribosomes have diverged significantly during evolution and present strikingly different structures across eukaryotic species(6-10). In contrast to animals and trypanosomatids, plant mitoribosomes have unusually expanded ribosomal RNAs and have conserved the short 5S rRNA, which is usually missing in mitoribosomes(11). We have previously characterized the composition of the plant mitoribosome(6), revealing a dozen plant-specific proteins in addition to the common conserved mitoribosomal proteins. In spite of the tremendous recent advances in the field, plant mitoribosomes remained elusive to high-resolution structural investigations and the plant-specific ribosomal features of unknown structures. Here, we present a cryo-electron microscopy study of the plant 78S mitoribosome from cauliflower at near-atomic resolution. We show that most of the plant-specific ribosomal proteins are pentatricopeptide repeat proteins (PPRs) that deeply interact with the plant-specific rRNA expansion segments. These additional rRNA segments and proteins reshape the overall structure of the plant mitochondrial ribosome, and we discuss their involvement in the membrane association and mRNA recruitment prior to translation initiation. Finally, our structure unveils an rRNA-constructive phase of mitoribosome evolution across eukaryotes.
		+
		+	Cryo-EM structure of the RNA-rich plant mitochondrial ribosome.,Waltz F, Soufari H, Bochler A, Giege P, Hashem Y Nat Plants. 2020 Apr;6(4):377-383. doi: 10.1038/s41477-020-0631-5. Epub 2020 Apr , 6. PMID:32251374<ref>PMID:32251374</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xyw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bochler A]]	+	[[Category: Bochler, A]]
-	[[Category: Giege P]]	+	[[Category: Giege, P]]
-	[[Category: Hashem Y]]	+	[[Category: Hashem, Y]]
-	[[Category: Soufari H]]	+	[[Category: Soufari, H]]
-	[[Category: Waltz F]]	+	[[Category: Waltz, F]]
		+	[[Category: Mitochondria]]
		+	[[Category: Plant]]
		+	[[Category: Ribosome]]

OCA at 07:05, 15 April 2020

OCA — Wed, 15 Apr 2020 07:05:34 GMT

←Older revision		Revision as of 07:05, 15 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xyw is ~~ON HOLD until Paper Publication~~	+	==Structure of the plant mitochondrial ribosome==
-		+	<StructureSection load='6xyw' size='340' side='right'caption='[[6xyw]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYW FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyw OCA], [http://pdbe.org/6xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyw RCSB], [http://www.ebi.ac.uk/pdbsum/6xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyw ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bochler A]]
		+	[[Category: Giege P]]
		+	[[Category: Hashem Y]]
		+	[[Category: Soufari H]]
		+	[[Category: Waltz F]]

OCA at 05:24, 19 February 2020

OCA — Wed, 19 Feb 2020 05:24:13 GMT

←Older revision		Revision as of 05:24, 19 February 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xyw is ON HOLD	+	The entry 6xyw is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xyw" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2020 02:52:09 GMT

Protected "6xyw" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:52, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xyw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 13 Feb 2020 02:52:08 GMT

New page: '''Unreleased structure''' The entry 6xyw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xyw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xyw - Revision history

OCA at 06:18, 22 April 2020

OCA at 07:05, 15 April 2020

OCA at 05:24, 19 February 2020

OCA: Protected "6xyw" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xyw is ON HOLD Authors: Description: Category: Unreleased Structures