6xzp - Revision history

OCA at 10:20, 22 May 2024

OCA — Wed, 22 May 2024 10:20:28 GMT

←Older revision		Revision as of 10:20, 22 May 2024
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-	====	+	==Influenza C virus polymerase in complex with chicken ANP32A - Subclass 4==
-	<StructureSection load='6xzp' size='340' side='right'caption='[[6xzp]]' scene=''>	+	<StructureSection load='6xzp' size='340' side='right'caption='[[6xzp]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xzp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Influenza_C_virus_(C/Johannesburg/1/66) Influenza C virus (C/Johannesburg/1/66)] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XZP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzp OCA], [https://pdbe.org/6xzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xzp RCSB], [https://www.ebi.ac.uk/pdbsum/6xzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzp OCA], [https://pdbe.org/6xzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xzp RCSB], [https://www.ebi.ac.uk/pdbsum/6xzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PB2_INCJH PB2_INCJH] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1 (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aquatic birds represent a vast reservoir from which new pandemic influenza A viruses can emerge(1). Influenza viruses contain a negative-sense segmented RNA genome that is transcribed and replicated by the viral heterotrimeric RNA polymerase (FluPol) in the context of viral ribonucleoprotein complexes(2,3). RNA polymerases of avian influenza A viruses (FluPolA) replicate viral RNA inefficiently in human cells because of species-specific differences in acidic nuclear phosphoprotein 32 (ANP32), a family of essential host proteins for FluPol activity(4). Host-adaptive mutations, particularly a glutamic-acid-to-lysine mutation at amino acid residue 627 (E627K) in the 627 domain of the PB2 subunit, enable avian FluPolA to overcome this restriction and efficiently replicate viral RNA in the presence of human ANP32 proteins. However, the molecular mechanisms of genome replication and the interplay with ANP32 proteins remain largely unknown. Here we report cryo-electron microscopy structures of influenza C virus polymerase (FluPolC) in complex with human and chicken ANP32A. In both structures, two FluPolC molecules form an asymmetric dimer bridged by the N-terminal leucine-rich repeat domain of ANP32A. The C-terminal low-complexity acidic region of ANP32A inserts between the two juxtaposed PB2 627 domains of the asymmetric FluPolA dimer, suggesting a mechanism for how the adaptive PB2(E627K) mutation enables the replication of viral RNA in mammalian hosts. We propose that this complex represents a replication platform for the viral RNA genome, in which one of the FluPol molecules acts as a replicase while the other initiates the assembly of the nascent replication product into a viral ribonucleoprotein complex.
		+
		+	Host ANP32A mediates the assembly of the influenza virus replicase.,Carrique L, Fan H, Walker AP, Keown JR, Sharps J, Staller E, Barclay WS, Fodor E, Grimes JM Nature. 2020 Nov;587(7835):638-643. doi: 10.1038/s41586-020-2927-z. Epub 2020 Nov , 18. PMID:33208942<ref>PMID:33208942</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xzp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Carrique L]]
		+	[[Category: Fan H]]
		+	[[Category: Fodor E]]
		+	[[Category: Grimes JM]]
		+	[[Category: Keown JR]]

OCA at 14:52, 17 June 2021

OCA — Thu, 17 Jun 2021 14:52:57 GMT

←Older revision		Revision as of 14:52, 17 June 2021
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	<StructureSection load='6xzp' size='340' side='right'caption='[[6xzp]]' scene=''>		<StructureSection load='6xzp' size='340' side='right'caption='[[6xzp]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzp OCA], [~~http~~://pdbe.org/6xzp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xzp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xzp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzp ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzp OCA], [https://pdbe.org/6xzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xzp RCSB], [https://www.ebi.ac.uk/pdbsum/6xzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzp ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 06:56, 25 November 2020

OCA — Wed, 25 Nov 2020 06:56:03 GMT

←Older revision		Revision as of 06:56, 25 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xzp ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xzp' size='340' side='right'caption='[[6xzp]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xzp OCA], [http://pdbe.org/6xzp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xzp RCSB], [http://www.ebi.ac.uk/pdbsum/6xzp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xzp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "6xzp" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2020 02:54:43 GMT

Protected "6xzp" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:54, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xzp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 13 Feb 2020 02:54:41 GMT

New page: '''Unreleased structure''' The entry 6xzp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xzp is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]