6y15 - Revision history

OCA at 13:16, 24 January 2024

OCA — Wed, 24 Jan 2024 13:16:59 GMT

←Older revision		Revision as of 13:16, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6y15' size='340' side='right'caption='[[6y15]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6y15' size='340' side='right'caption='[[6y15]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis Levilactobacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[6h07\|6h07]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">radh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [https://pdbe.org/6y15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [https://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [~~http~~://pdbe.org/6y15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q84EX5_LEVBR Q84EX5_LEVBR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus casei g von freudenreich and thoni 1904]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bischoff, D]]	+	[[Category: Levilactobacillus brevis]]
-	[[Category: Grob, P]]	+	[[Category: Bischoff D]]
-	[[Category: Hekmat, D]]	+	[[Category: Grob P]]
-	[[Category: Hermann, J]]	+	[[Category: Hekmat D]]
-	[[Category: Janowski, R]]	+	[[Category: Hermann J]]
-	[[Category: Niessing, D]]	+	[[Category: Janowski R]]
-	[[Category: Weuster-Botz, D]]	+	[[Category: Niessing D]]
-	~~[[Category: Nucleotide binding oxidoreductase activity]]~~	+	[[Category: Weuster-Botz D]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 12:53, 16 December 2020

OCA — Wed, 16 Dec 2020 12:53:26 GMT

←Older revision		Revision as of 12:53, 16 December 2020
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h07\|6h07]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6h07\|6h07]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">radh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">radh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [http://pdbe.org/6y15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [http://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [http://pdbe.org/6y15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [http://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>

OCA at 10:07, 12 August 2020

OCA — Wed, 12 Aug 2020 10:07:32 GMT

←Older revision		Revision as of 10:07, 12 August 2020
Line 3:		Line 3:
	<StructureSection load='6y15' size='340' side='right'caption='[[6y15]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6y15' size='340' side='right'caption='[[6y15]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y15 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h07\|6h07]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6h07\|6h07]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [http://pdbe.org/6y15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [http://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">radh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [http://pdbe.org/6y15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [http://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Technical crystallization is an attractive method to purify recombinant proteins. However, it is rarely applied due to the limited crystallizability of many proteins. To overcome this limitation, single amino acid exchanges are rationally introduced to enhance intermolecular interactions at the crystal contacts of the industrially relevant biocatalyst Lactobacillus brevis alcohol dehydrogenase (LbADH). The wildtype (WT) and the best crystallizing and enzymatically active LbADH mutants K32A, D54F, Q126H, and T102E are produced with Escherichia coli and subsequently crystallized from cell lysate in stirred mL-crystallizers. Notwithstanding the high host cell protein (HCP) concentrations in the lysate, all mutants crystallize significantly faster than the WT. Combinations of mutations result in double mutants with faster crystallization kinetics than the respective single mutants, demonstrating a synergetic effect. The almost entire depletion of the soluble LbADH fraction at crystallization equilibrium is observed, proving high yields. The HCP concentration is reduced to below 0.5% after crystal dissolution and recrystallization, and thus a 100-fold HCP reduction is achieved after two successive crystallization steps. The combination of fast kinetics, high yields, and high target protein purity highlights the potential of crystal contact engineering to transform technical crystallization into an efficient protein capture and purification step in biotechnological downstream processes.
		+
		+	Crystal Contact Engineering Enables Efficient Capture and Purification of an Oxidoreductase by Technical Crystallization.,Grob P, Huber M, Walla B, Hermann J, Janowski R, Niessing D, Hekmat D, Weuster-Botz D Biotechnol J. 2020 Apr 17:e2000010. doi: 10.1002/biot.202000010. PMID:32302461<ref>PMID:32302461</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6y15" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alcohol dehydrogenase 3D structures\|Alcohol dehydrogenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus casei g von freudenreich and thoni 1904]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bischoff, D]]		[[Category: Bischoff, D]]

OCA: Protected "6y15" [edit=sysop:move=sysop]

OCA — Wed, 19 Feb 2020 06:46:21 GMT

Protected "6y15" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:46, 19 February 2020

OCA: New page: ==X-ray structure of Lactobacillus brevis alcohol dehydrogenase mutant T102E_Q126K== == Structural highlights ==
<table><tr><td colspan='2'>[[6y15]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y15 OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Y15 FirstGlance]. 
</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl">[[Related_structure|Related:]]</td><td class="sblockDat">[[6h07|6h07]]</td></tr>
<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6y15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y15 OCA], [http://pdbe.org/6y15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y15 RCSB], [http://www.ebi.ac.uk/pdbsum/6y15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y15 ProSAT]</td></tr>
</table>
TOC
</StructureSection>
[[Category: Large Structures]]
[[Category: Bischoff, D]]
[[Category: Grob, P]]
[[Category: Hekmat, D]]
[[Category: Hermann, J]]
[[Category: Janowski, R]]
[[Category: Niessing, D]]
[[Category: Weuster-Botz, D]]
[[Category: Nucleotide binding oxidoreductase activity]]
[[Category: Oxidoreductase]]