6y4d - Revision history

OCA at 13:19, 24 January 2024

OCA — Wed, 24 Jan 2024 13:19:20 GMT

←Older revision		Revision as of 13:19, 24 January 2024
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	<StructureSection load='6y4d' size='340' side='right'caption='[[6y4d]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6y4d' size='340' side='right'caption='[[6y4d]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6y4d]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Zephyranthes_treatiae Zephyranthes treatiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6Y4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zephyranthes_treatiae Zephyranthes treatiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y4D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6y4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4d OCA], [~~http~~://pdbe.org/6y4d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6y4d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6y4d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4d ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4d OCA], [https://pdbe.org/6y4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y4d RCSB], [https://www.ebi.ac.uk/pdbsum/6y4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4d ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Zephyranthes treatiae]]		[[Category: Zephyranthes treatiae]]
-	[[Category: Mueller, M]]	+	[[Category: Mueller M]]
-	[[Category: Roth, S]]	+	[[Category: Roth S]]
-	[[Category: Sautner, V]]	+	[[Category: Sautner V]]
-	[[Category: Steimle, S]]	+	[[Category: Steimle S]]
-	[[Category: Tittmann, K]]	+	[[Category: Tittmann K]]
-	~~[[Category: Asymmetric reduction]]~~	+
-	~~[[Category: Biocatalysis]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Imine reduction]]~~	+
-	~~[[Category: Mutagenesis]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rational design]]~~	+
-	~~[[Category: Short-chain dehydrogenase/reductase~~]]	+

OCA at 11:31, 23 September 2020

OCA — Wed, 23 Sep 2020 11:31:53 GMT

←Older revision		Revision as of 11:31, 23 September 2020
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	==Crystal structure of a short-chain dehydrogenase/reductase (SDR) from Zephyranthes treatiae in complex with NADP+==		==Crystal structure of a short-chain dehydrogenase/reductase (SDR) from Zephyranthes treatiae in complex with NADP+==
-	<StructureSection load='6y4d' size='340' side='right'caption='[[6y4d]]' scene=''>	+	<StructureSection load='6y4d' size='340' side='right'caption='[[6y4d]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y4d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Zephyranthes_treatiae Zephyranthes treatiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y4D FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4d OCA], [http://pdbe.org/6y4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y4d RCSB], [http://www.ebi.ac.uk/pdbsum/6y4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4d ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4d OCA], [http://pdbe.org/6y4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y4d RCSB], [http://www.ebi.ac.uk/pdbsum/6y4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4d ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs) comprises numerous biocatalysts capable of C=O or C=C reduction. The highly homologous noroxomaritidine reductase (NR) from Narcissus sp. aff. pseudonarcissus and Zt_SDR from Zephyranthes treatiae, however, are SDRs with an extended imine substrate scope. Comparison with a similar SDR from Asparagus officinalis (Ao_SDR) exhibiting keto-reducing activity, yet negligible imine-reducing capability, and mining the Short-Chain Dehydrogenase/Reductase Engineering Database indicated that NR and Zt_SDR possess a unique active-site composition among SDRs. Adapting the active site of Ao_SDR accordingly improved its imine-reducing capability. By applying the same strategy, an unrelated SDR from Methylobacterium sp. 77 (M77_SDR) with distinct keto-reducing activity was engineered into a promiscuous enzyme with imine-reducing activity, thereby confirming that the ability to reduce imines can be rationally introduced into members of the "classical" SDR enzyme family. Thus, members of the SDR family could be a promising starting point for protein approaches to generate new imine-reducing enzymes.
		+
		+	Crossing the Border: From Keto- to Imine Reduction in Short-Chain Dehydrogenases/Reductases.,Roth S, Stockinger P, Steff J, Steimle S, Sautner V, Tittmann K, Pleiss J, Muller M Chembiochem. 2020 Sep 14;21(18):2615-2619. doi: 10.1002/cbic.202000233. Epub 2020, Jul 2. PMID:32315494<ref>PMID:32315494</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6y4d" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mueller M]]	+	[[Category: Zephyranthes treatiae]]
-	[[Category: Roth S]]	+	[[Category: Mueller, M]]
-	[[Category: Sautner V]]	+	[[Category: Roth, S]]
-	[[Category: Steimle S]]	+	[[Category: Sautner, V]]
-	[[Category: Tittmann K]]	+	[[Category: Steimle, S]]
		+	[[Category: Tittmann, K]]
		+	[[Category: Asymmetric reduction]]
		+	[[Category: Biocatalysis]]
		+	[[Category: Complex]]
		+	[[Category: Imine reduction]]
		+	[[Category: Mutagenesis]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rational design]]
		+	[[Category: Short-chain dehydrogenase/reductase]]

OCA at 06:51, 29 April 2020

OCA — Wed, 29 Apr 2020 06:51:22 GMT

←Older revision		Revision as of 06:51, 29 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6y4d is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of a short-chain dehydrogenase/reductase (SDR) from Zephyranthes treatiae in complex with NADP+==
-		+	<StructureSection load='6y4d' size='340' side='right'caption='[[6y4d]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y4D FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4d OCA], [http://pdbe.org/6y4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y4d RCSB], [http://www.ebi.ac.uk/pdbsum/6y4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4d ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mueller M]]
		+	[[Category: Roth S]]
		+	[[Category: Sautner V]]
		+	[[Category: Steimle S]]
		+	[[Category: Tittmann K]]

OCA at 05:41, 8 April 2020

OCA — Wed, 08 Apr 2020 05:41:13 GMT

←Older revision		Revision as of 05:41, 8 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6y4d is ON HOLD	+	The entry 6y4d is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6y4d" [edit=sysop:move=sysop]

OCA — Wed, 26 Feb 2020 08:21:53 GMT

Protected "6y4d" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:21, 26 February 2020

OCA: New page: '''Unreleased structure''' The entry 6y4d is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Feb 2020 08:21:52 GMT

New page: '''Unreleased structure''' The entry 6y4d is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6y4d is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]