6y5v - Revision history

OCA at 10:43, 28 July 2021

OCA — Wed, 28 Jul 2021 10:43:09 GMT

←Older revision		Revision as of 10:43, 28 July 2021
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	==Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/T997D) in KCl==		==Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/T997D) in KCl==
-	<StructureSection load='6y5v' size='340' side='right'caption='[[6y5v]]' scene=''>	+	<StructureSection load='6y5v' size='340' side='right'caption='[[6y5v]], [[Resolution\|resolution]] 4.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y5V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y5v OCA], [https://pdbe.org/6y5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y5v RCSB], [https://www.ebi.ac.uk/pdbsum/6y5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y5v ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SLC12A6, KCC3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y5v OCA], [https://pdbe.org/6y5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y5v RCSB], [https://www.ebi.ac.uk/pdbsum/6y5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y5v ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Corpus callosum agenesis-neuronopathy syndrome. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/S12A6_HUMAN S12A6_HUMAN]] Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Potassium-coupled chloride transporters (KCCs) play crucial roles in regulating cell volume and intracellular chloride concentration. They are characteristically inhibited under isotonic conditions via phospho-regulatory sites located within the cytoplasmic termini. Decreased inhibitory phosphorylation in response to hypotonic cell swelling stimulates transport activity, and dysfunction of this regulatory process has been associated with various human diseases. Here, we present cryo-EM structures of human KCC3b and KCC1, revealing structural determinants for phospho-regulation in both N- and C-termini. We show that phospho-mimetic KCC3b is arrested in an inward-facing state in which intracellular ion access is blocked by extensive contacts with the N-terminus. In another mutant with increased isotonic transport activity, KCC1Delta19, this interdomain interaction is absent, likely due to a unique phospho-regulatory site in the KCC1 N-terminus. Furthermore, we map additional phosphorylation sites as well as a previously unknown ATP/ADP-binding pocket in the large C-terminal domain and show enhanced thermal stabilization of other CCCs by adenine nucleotides. These findings provide fundamentally new insights into the complex regulation of KCCs and may unlock innovative strategies for drug development.
		+
		+	Phospho-regulation, nucleotide binding and ion access control in potassium-chloride cotransporters.,Chi G, Ebenhoch R, Man H, Tang H, Tremblay LE, Reggiano G, Qiu X, Bohstedt T, Liko I, Almeida FG, Garneau AP, Wang D, McKinley G, Moreau CP, Bountra KD, Abrusci P, Mukhopadhyay SMM, Fernandez-Cid A, Slimani S, Lavoie JL, Burgess-Brown NA, Tehan B, DiMaio F, Jazayeri A, Isenring P, Robinson CV, Durr KL EMBO J. 2021 May 25:e107294. doi: 10.15252/embj.2020107294. PMID:34031912<ref>PMID:34031912</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6y5v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abrusci P]]	+	[[Category: Abrusci, P]]
-	[[Category: Arrowsmith CH]]	+	[[Category: Arrowsmith, C H]]
-	[[Category: Bohstedt T]]	+	[[Category: Bohstedt, T]]
-	[[Category: Bountra C]]	+	[[Category: Bountra, C]]
-	[[Category: Burgess-Brown NA]]	+	[[Category: Burgess-Brown, N A]]
-	[[Category: Chalk R]]	+	[[Category: Chalk, R]]
-	[[Category: Chi G]]	+	[[Category: Chi, G]]
-	[[Category: DiMaio F]]	+	[[Category: DiMaio, F]]
-	[[Category: Duerr KL]]	+	[[Category: Duerr, K L]]
-	[[Category: Ebenhoch R]]	+	[[Category: Ebenhoch, R]]
-	[[Category: Edwards AM]]	+	[[Category: Edwards, A M]]
-	[[Category: MacLean EM]]	+	[[Category: MacLean, E M]]
-	[[Category: Man H]]	+	[[Category: Man, H]]
-	[[Category: Marsden BD]]	+	[[Category: Marsden, B D]]
-	[[Category: McKinley G]]	+	[[Category: McKinley, G]]
-	[[Category: Moreau C]]	+	[[Category: Moreau, C]]
-	[[Category: Mukhopadhyay ~~SMM~~]]	+	[[Category: Mukhopadhyay, S M.M]]
-	[[Category: Pike ~~ACW~~]]	+	[[Category: Pike, A C.W]]
-	[[Category: Reggiano G]]	+	[[Category: Reggiano, G]]
-	[[Category: Singh NK]]	+	[[Category: Singh, N K]]
-	[[Category: Snee M]]	+	[[Category: Snee, M]]
-	[[Category: Wang D]]	+	[[Category: Wang, D]]
		+	[[Category: Apc]]
		+	[[Category: Dimer]]
		+	[[Category: Kcc3]]
		+	[[Category: Membrane protein]]
		+	[[Category: Potassium chloride]]
		+	[[Category: Sgc]]
		+	[[Category: Slc12a6]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transporter]]

OCA at 12:26, 9 June 2021

OCA — Wed, 09 Jun 2021 12:26:07 GMT

←Older revision		Revision as of 12:26, 9 June 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6y5v ~~is ON HOLD~~	+	==Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/T997D) in KCl==
-		+	<StructureSection load='6y5v' size='340' side='right'caption='[[6y5v]]' scene=''>
-	~~Authors~~: ~~Chi, G~~.~~, Man, H~~.~~, Ebenhoch, R~~.~~, Reggiano, G~~.~~, Pike, A~~.C.W.~~, Wang, D~~.~~, McKinley, G~~., ~~Mukhopadhyay, S~~.M.M., ~~MacLean, E.M~~., ~~Chalk, R~~.~~, Moreau, C~~.~~, Snee, M~~., ~~Bohstedt, T~~.~~, Singh, N~~.K., ~~Abrusci, P~~.~~, Arrowsmith, C.H., Bountra, C., Edwards, A.M., Marsden, B.D., Burgess~~-~~Brown, N.A., DiMaio, F., Duerr, K.L~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y5V FirstGlance]. <br>
-	~~Description: Structure of Human Potassium Chloride Transporter KCC3b (S45D~~/~~T940D/T997D) in KCl~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y5v OCA], [https://pdbe.org/6y5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y5v RCSB], [https://www.ebi.ac.uk/pdbsum/6y5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y5v ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Marsden, B.D~~]]	+	__TOC__
-	[[Category: ~~Pike, A.C.W~~]]	+	</StructureSection>
-	[[Category: ~~Arrowsmith, C.H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Chalk, R~~]]	+	[[Category: Abrusci P]]
-	[[Category: ~~Singh, N.K~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: ~~Moreau, C~~]]	+	[[Category: Bohstedt T]]
-	[[Category: ~~Man, H~~]]	+	[[Category: Bountra C]]
-	[[Category: ~~Burgess-Brown, N.A~~]]	+	[[Category: Burgess-Brown NA]]
-	[[Category: ~~Wang, D~~]]	+	[[Category: Chalk R]]
-	[[Category: ~~Chi, G~~]]	+	[[Category: Chi G]]
-	[[Category: ~~Duerr, K.L~~]]	+	[[Category: DiMaio F]]
-	[[Category: ~~Ebenhoch, R~~]]	+	[[Category: Duerr KL]]
-	[[Category: ~~Abrusci, P~~]]	+	[[Category: Ebenhoch R]]
-	[[Category: ~~Mukhopadhyay, S.M.M~~]]	+	[[Category: Edwards AM]]
-	[[Category: ~~Edwards, A.M~~]]	+	[[Category: MacLean EM]]
-	[[Category: ~~Dimaio, F~~]]	+	[[Category: Man H]]
-	[[Category: ~~Maclean, E.M~~]]	+	[[Category: Marsden BD]]
-	[[Category: ~~Reggiano, G~~]]	+	[[Category: McKinley G]]
-	[[Category: ~~Snee, M~~]]	+	[[Category: Moreau C]]
-	[[Category: ~~Bohstedt, T~~]]	+	[[Category: Mukhopadhyay SMM]]
-	[[Category: ~~Bountra, C~~]]	+	[[Category: Pike ACW]]
-	[[Category: ~~Mckinley, G~~]]	+	[[Category: Reggiano G]]
		+	[[Category: Singh NK]]
		+	[[Category: Snee M]]
		+	[[Category: Wang D]]

OCA at 07:38, 1 April 2020

OCA — Wed, 01 Apr 2020 07:38:37 GMT

←Older revision		Revision as of 07:38, 1 April 2020
Line 3:		Line 3:
	The entry 6y5v is ON HOLD		The entry 6y5v is ON HOLD

-	Authors: Chi, G., Man, H., Ebenhoch, R., Reggiano, G., Pike, A.C.W., Wang, D., McKinley, G., Mukhopadhyay, S.M.M., MacLean, B., Chalk, R., Moreau, C., Snee, M., Bohstedt, T., Singh, N.K., Abrusci, P., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Marsden, B.D., Burgess-Brown, N.A., DiMaio, F., Duerr, K.L.	+	Authors: Chi, G., Man, H., Ebenhoch, R., Reggiano, G., Pike, A.C.W., Wang, D., McKinley, G., Mukhopadhyay, S.M.M., MacLean, E.M., Chalk, R., Moreau, C., Snee, M., Bohstedt, T., Singh, N.K., Abrusci, P., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Marsden, B.D., Burgess-Brown, N.A., DiMaio, F., Duerr, K.L.

-	Description: Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/~~T991D~~) in KCl	+	Description: Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/T997D) in KCl
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Marsden, B.D]]		[[Category: Marsden, B.D]]
Line 20:		Line 20:
	[[Category: Ebenhoch, R]]		[[Category: Ebenhoch, R]]
	[[Category: Abrusci, P]]		[[Category: Abrusci, P]]
-	[[Category: Maclean, B]]
	[[Category: Mukhopadhyay, S.M.M]]		[[Category: Mukhopadhyay, S.M.M]]
	[[Category: Edwards, A.M]]		[[Category: Edwards, A.M]]
	[[Category: Dimaio, F]]		[[Category: Dimaio, F]]
		+	[[Category: Maclean, E.M]]
	[[Category: Reggiano, G]]		[[Category: Reggiano, G]]
	[[Category: Snee, M]]		[[Category: Snee, M]]

OCA: Protected "6y5v" [edit=sysop:move=sysop]

OCA — Wed, 04 Mar 2020 05:45:42 GMT

Protected "6y5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:45, 4 March 2020

OCA: New page: '''Unreleased structure''' The entry 6y5v is ON HOLD Authors: Chi, G., Man, H., Ebenhoch, R., Reggiano, G., Pike, A.C.W., Wang, D., McKinley, G., Mukhopadhyay, S.M.M., MacLean, B., Chal...

OCA — Wed, 04 Mar 2020 05:45:41 GMT

New page: '''Unreleased structure''' The entry 6y5v is ON HOLD Authors: Chi, G., Man, H., Ebenhoch, R., Reggiano, G., Pike, A.C.W., Wang, D., McKinley, G., Mukhopadhyay, S.M.M., MacLean, B., Chal...

New page

'''Unreleased structure'''

The entry 6y5v is ON HOLD

Authors: Chi, G., Man, H., Ebenhoch, R., Reggiano, G., Pike, A.C.W., Wang, D., McKinley, G., Mukhopadhyay, S.M.M., MacLean, B., Chalk, R., Moreau, C., Snee, M., Bohstedt, T., Singh, N.K., Abrusci, P., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Marsden, B.D., Burgess-Brown, N.A., DiMaio, F., Duerr, K.L.

Description: Structure of Human Potassium Chloride Transporter KCC3b (S45D/T940D/T991D) in KCl
[[Category: Unreleased Structures]]
[[Category: Marsden, B.D]]
[[Category: Pike, A.C.W]]
[[Category: Arrowsmith, C.H]]
[[Category: Chalk, R]]
[[Category: Singh, N.K]]
[[Category: Moreau, C]]
[[Category: Man, H]]
[[Category: Burgess-Brown, N.A]]
[[Category: Wang, D]]
[[Category: Chi, G]]
[[Category: Duerr, K.L]]
[[Category: Ebenhoch, R]]
[[Category: Abrusci, P]]
[[Category: Maclean, B]]
[[Category: Mukhopadhyay, S.M.M]]
[[Category: Edwards, A.M]]
[[Category: Dimaio, F]]
[[Category: Reggiano, G]]
[[Category: Snee, M]]
[[Category: Bohstedt, T]]
[[Category: Bountra, C]]
[[Category: Mckinley, G]]