6y7t - Revision history

OCA at 13:21, 24 January 2024

OCA — Wed, 24 Jan 2024 13:21:16 GMT

←Older revision		Revision as of 13:21, 24 January 2024
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-	====	+	==Engineered conjugation of lysine-specific molecular tweezers with ExoS derived peptidic inhibitor enhance affinity towards target protein 14-3-3 through ditopic binding==
-	<StructureSection load='6y7t' size='340' side='right'caption='[[6y7t]]' scene=''>	+	<StructureSection load='6y7t' size='340' side='right'caption='[[6y7t]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6y7t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y7T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y7t OCA], [https://pdbe.org/6y7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y7t RCSB], [https://www.ebi.ac.uk/pdbsum/6y7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y7t ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TWZ:ExoSTWZ+molecular+tweezer'>TWZ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y7t OCA], [https://pdbe.org/6y7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y7t RCSB], [https://www.ebi.ac.uk/pdbsum/6y7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y7t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rational design of protein-protein interaction (PPI) inhibitors is challenging. Connecting a general supramolecular protein binder with a specific peptidic ligand provides a novel conceptual approach. Thus, lysine-specific molecular tweezers were conjugated to a peptide-based 14-3-3 ligand and produced a strong PPI inhibitor with 100-fold elevated protein affinity. X-ray crystal structure elucidation of this supramolecular directed assembly provides unique molecular insight into the binding mode and fully aligns with Molecular Dynamics (MD) simulations. This new supramolecular chemical biology concept opens the path to novel chemical tools for studying PPIs.
		+
		+	Supramolecular Enhancement of a Natural 14-3-3 Protein Ligand.,Guillory X, Hadrovic I, de Vink PJ, Sowislok A, Brunsveld L, Schrader T, Ottmann C J Am Chem Soc. 2021 Sep 1;143(34):13495-13500. doi: 10.1021/jacs.1c07095. Epub , 2021 Aug 24. PMID:34427424<ref>PMID:34427424</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6y7t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Guillory X]]
		+	[[Category: Ottmann C]]

OCA at 10:29, 31 March 2021

OCA — Wed, 31 Mar 2021 10:29:33 GMT

←Older revision		Revision as of 10:29, 31 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6y7t ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6y7t' size='340' side='right'caption='[[6y7t]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y7t OCA], [https://pdbe.org/6y7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y7t RCSB], [https://www.ebi.ac.uk/pdbsum/6y7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y7t ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:12, 22 July 2020

OCA — Wed, 22 Jul 2020 07:12:50 GMT

←Older revision		Revision as of 07:12, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6y7t is ON HOLD	+	The entry 6y7t is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 08:31, 27 March 2020

OCA — Fri, 27 Mar 2020 08:31:31 GMT

←Older revision		Revision as of 08:31, 27 March 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6y7t is ON HOLD ~~until sometime in the future~~	+	The entry 6y7t is ON HOLD

	Authors:		Authors:

OCA: Protected "6y7t" [edit=sysop:move=sysop]

OCA — Wed, 11 Mar 2020 06:16:16 GMT

Protected "6y7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:16, 11 March 2020

OCA: New page: '''Unreleased structure''' The entry 6y7t is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Mar 2020 06:16:14 GMT

New page: '''Unreleased structure''' The entry 6y7t is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6y7t is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]