6ya1 - Revision history

OCA at 13:22, 24 January 2024

OCA — Wed, 24 Jan 2024 13:22:44 GMT

←Older revision		Revision as of 13:22, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6ya1' size='340' side='right'caption='[[6ya1]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='6ya1' size='340' side='right'caption='[[6ya1]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ya1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_33152 Atcc 33152~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YA1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ya1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YA1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ya1 OCA], [https://pdbe.org/6ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ya1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ya1 OCA], [https://pdbe.org/6ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ya1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PROA_LEGPN PROA_LEGPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33152]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Blankenfeldt, W~~]]	+	[[Category: Legionella pneumophila]]
-	[[Category: ~~Schmelz, S~~]]	+	[[Category: Blankenfeldt W]]
-	[[Category: ~~M4 protease]]~~	+	[[Category: Schmelz S]]
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Proa]]~~	+
-	~~[[Category: Tlp-like]]~~	+
-	~~[[Category: Zinc~~]]	+

OCA at 08:29, 21 April 2021

OCA — Wed, 21 Apr 2021 08:29:31 GMT

←Older revision		Revision as of 08:29, 21 April 2021
Line 1:		Line 1:

	==Zinc metalloprotease ProA==		==Zinc metalloprotease ProA==
-	<StructureSection load='6ya1' size='340' side='right'caption='[[6ya1]]' scene=''>	+	<StructureSection load='6ya1' size='340' side='right'caption='[[6ya1]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YA1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ya1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YA1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ya1 OCA], [https://pdbe.org/6ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ya1 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ya1 OCA], [https://pdbe.org/6ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ya1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ProA is a secreted zinc metalloprotease of Legionella pneumophila causing lung damage in animal models of Legionnaires' disease. Here we demonstrate that ProA promotes infection of human lung tissue explants (HLTEs) and dissect the contribution to cell type specific replication and extracellular virulence mechanisms. For the first time, we reveal that co-incubation of HLTEs with purified ProA causes a significant increase of the alveolar septal thickness. This destruction of connective tissue fibers was further substantiated by collagen IV degradation assays. The moderate attenuation of a proA-negative mutant in A549 epithelial cells and THP-1 macrophages suggest that effects of ProA in tissue mainly result from extracellular activity. Correspondingly, ProA contributes to dissemination and serum resistance of the pathogen, which further expands the versatile substrate spectrum of this thermolysin-like protease. The crystal structure of ProA at 1.48 a resolution showed high congruence to pseudolysin of Pseudomonas aeruginosa, but revealed deviations in flexible loops, the substrate binding pocket S1 ' and the repertoire of cofactors, by which ProA can be distinguished from respective homologs. In sum, this work specified virulence features of ProA at different organizational levels by zooming in from histopathological effects in human lung tissue to atomic details of the protease substrate determination. This article is protected by copyright. All rights reserved.
		+
		+	Zinc metalloprotease ProA of Legionella pneumophila increases alveolar septal thickness in human lung tissue explants by collagen IV degradation.,Scheithauer L, Thiem S, Schmelz S, Dellmann A, Bussow K, Brouwer RMHJ, Unal CM, Blankenfeldt W, Steinert M Cell Microbiol. 2021 Jan 24:e13313. doi: 10.1111/cmi.13313. PMID:33491325<ref>PMID:33491325</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ya1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33152]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blankenfeldt W]]	+	[[Category: Blankenfeldt, W]]
-	[[Category: Schmelz S]]	+	[[Category: Schmelz, S]]
		+	[[Category: M4 protease]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Proa]]
		+	[[Category: Tlp-like]]
		+	[[Category: Zinc]]

OCA at 11:08, 3 February 2021

OCA — Wed, 03 Feb 2021 11:08:49 GMT

←Older revision		Revision as of 11:08, 3 February 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ya1 ~~is ON HOLD until Mar 11 2022~~	+	==Zinc metalloprotease ProA==
-		+	<StructureSection load='6ya1' size='340' side='right'caption='[[6ya1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YA1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ya1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ya1 OCA], [https://pdbe.org/6ya1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ya1 RCSB], [https://www.ebi.ac.uk/pdbsum/6ya1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ya1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Blankenfeldt W]]
		+	[[Category: Schmelz S]]

OCA at 07:14, 22 July 2020

OCA — Wed, 22 Jul 2020 07:14:49 GMT

←Older revision		Revision as of 07:14, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ya1 is ON HOLD	+	The entry 6ya1 is ON HOLD until Mar 11 2022

	Authors:		Authors:

OCA: Protected "6ya1" [edit=sysop:move=sysop]

OCA — Wed, 18 Mar 2020 08:40:27 GMT

Protected "6ya1" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:40, 18 March 2020

OCA: New page: '''Unreleased structure''' The entry 6ya1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Mar 2020 08:40:26 GMT

New page: '''Unreleased structure''' The entry 6ya1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ya1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]