6yb9 - Revision history

OCA at 13:23, 24 January 2024

OCA — Wed, 24 Jan 2024 13:23:21 GMT

←Older revision		Revision as of 13:23, 24 January 2024
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	<StructureSection load='6yb9' size='340' side='right'caption='[[6yb9]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='6yb9' size='340' side='right'caption='[[6yb9]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6yb9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YB9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6YB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yb9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YB9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OK8:(2~{S})-2-[[5-azanyl-1-[(2~{R},3~{R},4~{S},5~{R})-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]imidazol-4-yl]car+bonylamino]butanedioic+acid'>OK8</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.406Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6yb8\|6yb8]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OK8:(2~{S})-2-[[5-azanyl-1-[(2~{R},3~{R},4~{S},5~{R})-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]imidazol-4-yl]car+bonylamino]butanedioic+acid'>OK8</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAICS, ADE2, AIRC, PAIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yb9 OCA], [https://pdbe.org/6yb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yb9 RCSB], [https://www.ebi.ac.uk/pdbsum/6yb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yb9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6yb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yb9 OCA], [~~http~~://pdbe.org/6yb9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6yb9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6yb9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6yb9 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PUR6_HUMAN PUR6_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gottmann, M]]	+	[[Category: Gottmann M]]
-	[[Category: Helleday, T]]	+	[[Category: Helleday T]]
-	[[Category: Homan, E]]	+	[[Category: Homan E]]
-	[[Category: Jemth~~, A S~~]]	+	[[Category: Jemth AS]]
-	[[Category: Skerlova, J]]	+	[[Category: Skerlova J]]
-	[[Category: Stenmark, P]]	+	[[Category: Stenmark P]]
-	[[Category: Unterlass, J]]	+	[[Category: Unterlass J]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Cancer target]]~~	+
-	~~[[Category: De novo purine biosynthesis]]~~	+
-	~~[[Category: Nucleotide metabolism~~]]	+

OCA at 07:11, 2 September 2020

OCA — Wed, 02 Sep 2020 07:11:49 GMT

←Older revision		Revision as of 07:11, 2 September 2020
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	==Human octameric PAICS in complex with SAICAR, AMP-PNP, and magnesium==		==Human octameric PAICS in complex with SAICAR, AMP-PNP, and magnesium==
-	<StructureSection load='6yb9' size='340' side='right'caption='[[6yb9]]' scene=''>	+	<StructureSection load='6yb9' size='340' side='right'caption='[[6yb9]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YB9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YB9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yb9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YB9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YB9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yb9 OCA], [http://pdbe.org/6yb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yb9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yb9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OK8:(2~{S})-2-[[5-azanyl-1-[(2~{R},3~{R},4~{S},5~{R})-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]imidazol-4-yl]car+bonylamino]butanedioic+acid'>OK8</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6yb8\|6yb8]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAICS, ADE2, AIRC, PAIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yb9 OCA], [http://pdbe.org/6yb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yb9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yb9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bifunctional human enzyme phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (PAICS) catalyzes two essential steps in the de novo purine biosynthesis pathway. PAICS is overexpressed in many cancers and could be a promising target for the development of cancer therapeutics. Here, using gene knockdowns and clonogenic survival and cell viability assays, we demonstrate that PAICS is required for growth and survival of prostate cancer cells. PAICS catalyzes the carboxylation of aminoimidazole ribonucleotide (AIR) and the subsequent conversion of carboxyaminoimidazole ribonucleotide (CAIR) and L-aspartate to N-succinylcarboxamide-5-aminoimidazole ribonucleotide (SAICAR). Of note, we present the first structures of human octameric PAICS in complexes with native ligands. In particular, we report the structure of PAICS with CAIR bound in the active sites of both domains and SAICAR bound in one of the SAICAR synthetase domains. Moreover, we report the PAICS structure with SAICAR and an ATP analog occupying the SAICAR synthetase active site. These structures provide insight into substrate and product binding and the architecture of the active sites, disclosing important structural information for rational design of PAICS inhibitors as potential anticancer drugs.
		+
		+	Crystal structures of human PAICS reveal substrate and product binding of an emerging cancer target.,Skerlova J, Unterlass J, Gottmann M, Marttila P, Homan E, Helleday T, Jemth AS, Stenmark P J Biol Chem. 2020 Jun 22. pii: RA120.013695. doi: 10.1074/jbc.RA120.013695. PMID:32571877<ref>PMID:32571877</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yb9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gottmann M]]	+	[[Category: Gottmann, M]]
-	[[Category: Helleday T]]	+	[[Category: Helleday, T]]
-	[[Category: Homan E]]	+	[[Category: Homan, E]]
-	[[Category: Jemth AS]]	+	[[Category: Jemth, A S]]
-	[[Category: Skerlova J]]	+	[[Category: Skerlova, J]]
-	[[Category: Stenmark P]]	+	[[Category: Stenmark, P]]
-	[[Category: Unterlass J]]	+	[[Category: Unterlass, J]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Cancer target]]
		+	[[Category: De novo purine biosynthesis]]
		+	[[Category: Nucleotide metabolism]]

OCA at 14:11, 8 July 2020

OCA — Wed, 08 Jul 2020 14:11:48 GMT

←Older revision		Revision as of 14:11, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6yb9 is ~~ON HOLD until Paper Publication~~	+	==Human octameric PAICS in complex with SAICAR, AMP-PNP, and magnesium==
-		+	<StructureSection load='6yb9' size='340' side='right'caption='[[6yb9]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YB9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YB9 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yb9 OCA], [http://pdbe.org/6yb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yb9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yb9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gottmann M]]
		+	[[Category: Helleday T]]
		+	[[Category: Homan E]]
		+	[[Category: Jemth AS]]
		+	[[Category: Skerlova J]]
		+	[[Category: Stenmark P]]
		+	[[Category: Unterlass J]]

OCA at 07:39, 1 April 2020

OCA — Wed, 01 Apr 2020 07:39:38 GMT

←Older revision		Revision as of 07:39, 1 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yb9 is ON HOLD	+	The entry 6yb9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6yb9" [edit=sysop:move=sysop]

OCA — Fri, 27 Mar 2020 08:34:37 GMT

Protected "6yb9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:34, 27 March 2020

OCA: New page: '''Unreleased structure''' The entry 6yb9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Fri, 27 Mar 2020 08:34:36 GMT

New page: '''Unreleased structure''' The entry 6yb9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6yb9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]