6yd5 - Revision history

OCA at 13:24, 24 January 2024

OCA — Wed, 24 Jan 2024 13:24:31 GMT

←Older revision		Revision as of 13:24, 24 January 2024
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	==SaFtsZ-UCM151 (comp. 18)==		==SaFtsZ-UCM151 (comp. 18)==
-	<StructureSection load='6yd5' size='340' side='right'caption='[[6yd5]]' scene=''>	+	<StructureSection load='6yd5' size='340' side='right'caption='[[6yd5]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YD5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yd5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YD5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yd5 OCA], [https://pdbe.org/6yd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yd5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yd5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MB3:1-METHYLPYRROLIDIN-2-ONE'>MB3</scene>, <scene name='pdbligand=OM8:3-[(3-chlorophenyl)methoxy]-2,6-bis(fluoranyl)benzamide'>OM8</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yd5 OCA], [https://pdbe.org/6yd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yd5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yd5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FTSZ_STAA8 FTSZ_STAA8] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]<ref>PMID:14617148</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial resistance to antibiotics makes previously manageable infections again disabling and lethal, highlighting the need for new antibacterial strategies. In this regard, inhibition of the bacterial division process by targeting key protein FtsZ has been recognized as an attractive approach for discovering new antibiotics. Binding of small molecules to the cleft between the N-terminal guanosine triphosphate (GTP)-binding and the C-terminal subdomains allosterically impairs the FtsZ function, eventually inhibiting bacterial division. Nonetheless, the lack of appropriate chemical tools to develop a binding screen against this site has hampered the discovery of FtsZ antibacterial inhibitors. Herein, we describe the first competitive binding assay to identify FtsZ allosteric ligands interacting with the interdomain cleft, based on the use of specific high-affinity fluorescent probes. This novel assay, together with phenotypic profiling and X-ray crystallographic insights, enables the identification and characterization of FtsZ inhibitors of bacterial division aiming at the discovery of more effective antibacterials.
		+
		+	Targeting the FtsZ Allosteric Binding Site with a Novel Fluorescence Polarization Screen, Cytological and Structural Approaches for Antibacterial Discovery.,Huecas S, Araujo-Bazan L, Ruiz FM, Ruiz-Avila LB, Martinez RF, Escobar-Pena A, Artola M, Vazquez-Villa H, Martin-Fontecha M, Fernandez-Tornero C, Lopez-Rodriguez ML, Andreu JM J Med Chem. 2021 May 13;64(9):5730-5745. doi: 10.1021/acs.jmedchem.0c02207. Epub , 2021 Apr 28. PMID:33908781<ref>PMID:33908781</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yd5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus]]
	[[Category: Andreu JM]]		[[Category: Andreu JM]]
	[[Category: Fernandez-Tornero C]]		[[Category: Fernandez-Tornero C]]
	[[Category: Ruiz FM]]		[[Category: Ruiz FM]]

OCA at 10:31, 28 July 2021

OCA — Wed, 28 Jul 2021 10:31:13 GMT

←Older revision		Revision as of 10:31, 28 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6yd5 ~~is ON HOLD until Paper Publication~~	+	==SaFtsZ-UCM151 (comp. 18)==
-		+	<StructureSection load='6yd5' size='340' side='right'caption='[[6yd5]]' scene=''>
-	~~Authors~~: ~~Fernandez~~-~~Tornero~~, C., ~~Ruiz~~, F.M., ~~Andreu, J~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YD5 FirstGlance]. <br>
-	~~Description~~: ~~SaFtsZ~~-~~UCM151 (comp~~. ~~18)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yd5 OCA], [https://pdbe.org/6yd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yd5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yd5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Ruiz, F.M~~]]	+	__TOC__
-	[[Category: Fernandez-Tornero, C]]	+	</StructureSection>
-	[[Category: ~~Andreu, J.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Andreu JM]]
		+	[[Category: Fernandez-Tornero C]]
		+	[[Category: Ruiz FM]]

OCA at 05:13, 17 March 2021

OCA — Wed, 17 Mar 2021 05:13:38 GMT

←Older revision		Revision as of 05:13, 17 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yd5 is ON HOLD	+	The entry 6yd5 is ON HOLD until Paper Publication

	Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M.		Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M.

OCA at 13:05, 3 March 2021

OCA — Wed, 03 Mar 2021 13:05:11 GMT

←Older revision		Revision as of 13:05, 3 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yd5 is ON HOLD ~~until Paper Publication~~	+	The entry 6yd5 is ON HOLD

-	Authors: Fernandez-Tornero, C., Andreu, J.M.	+	Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M.

-	Description: SaFtsZ-UCM151	+	Description: SaFtsZ-UCM151 (comp. 18)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Andreu~~, J.M]]	+	[[Category: Ruiz, F.M]]
	[[Category: Fernandez-Tornero, C]]		[[Category: Fernandez-Tornero, C]]
		+	[[Category: Andreu, J.M]]

OCA at 07:18, 22 July 2020

OCA — Wed, 22 Jul 2020 07:18:01 GMT

←Older revision		Revision as of 07:18, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yd5 is ON HOLD	+	The entry 6yd5 is ON HOLD until Paper Publication

	Authors: Fernandez-Tornero, C., Andreu, J.M.		Authors: Fernandez-Tornero, C., Andreu, J.M.

OCA: Protected "6yd5" [edit=sysop:move=sysop]

OCA — Fri, 27 Mar 2020 08:40:39 GMT

Protected "6yd5" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:40, 27 March 2020

OCA: New page: '''Unreleased structure''' The entry 6yd5 is ON HOLD Authors: Fernandez-Tornero, C., Andreu, J.M. Description: SaFtsZ-UCM151 Category: Unreleased Structures [[Category: Andreu, J.M...

OCA — Fri, 27 Mar 2020 08:40:37 GMT

New page: '''Unreleased structure''' The entry 6yd5 is ON HOLD Authors: Fernandez-Tornero, C., Andreu, J.M. Description: SaFtsZ-UCM151 Category: Unreleased Structures [[Category: Andreu, J.M...

New page

'''Unreleased structure'''

The entry 6yd5 is ON HOLD

Authors: Fernandez-Tornero, C., Andreu, J.M.

Description: SaFtsZ-UCM151
[[Category: Unreleased Structures]]
[[Category: Andreu, J.M]]
[[Category: Fernandez-Tornero, C]]