OCA at 13:30, 24 January 2024

OCA — Wed, 24 Jan 2024 13:30:45 GMT

←Older revision		Revision as of 13:30, 24 January 2024
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-	====	+	==Imidazole Glycerol Phosphate Synthase==
-	<StructureSection load='6ymu' size='340' side='right'caption='[[6ymu]]' scene=''>	+	<StructureSection load='6ymu' size='340' side='right'caption='[[6ymu]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ymu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YMU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ymu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ymu OCA], [~~http~~://pdbe.org/6ymu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ymu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ymu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ymu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.11Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OZW:phenylhydrazinyl+phenylalanine'>OZW</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ymu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ymu OCA], [https://pdbe.org/6ymu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ymu RCSB], [https://www.ebi.ac.uk/pdbsum/6ymu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ymu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Imidazole glycerol phosphate synthase (ImGPS) from Thermotoga maritima is a model enzyme for studying allostery. The ImGPS complex consists of the cyclase subunit HisF and the glutaminase subunit HisH whose activity is stimulated by substrate binding to HisF in a V-type manner. To investigate the significance of a putative closing hinge motion at the cyclase:glutaminase interface for HisH activity, we replaced residue W123 in HisH with the light-switchable unnatural amino acid phenylalanine-4'-azobenzene (AzoF). Crystal structure analysis employing angle, buried surface area, and distance measurements showed that incorporation of AzoF at this position causes a closing of the interface by approximately 18 +/- 3%. This slightly different interface configuration results in a much higher catalytic efficiency in unstimulated HisH due to an elevated turnover number. Moreover, the catalytic efficiency of HisH when stimulated by binding of a substrate to HisF was also significantly increased by AzoF incorporation. This was caused by a K-type stimulation that led to a decrease in the apparent dissociation constant for its substrate, glutamine. In addition, AzoF improved the apparent binding of a substrate analogue at the HisF active site. Remarkably, light-induced isomerization of AzoF considerably enhanced these effects. In conclusion, our findings confirm that signal transduction from HisF to HisH in ImGPS involves the closing of the cyclase:glutaminase subunit interface and that incorporation of AzoF at a hinge position reinforces this catalytically relevant conformational change.
		+
		+	Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase.,Kneuttinger AC, Rajendran C, Simeth NA, Bruckmann A, Konig B, Sterner R Biochemistry. 2020 Jul 13. doi: 10.1021/acs.biochem.0c00332. PMID:32633500<ref>PMID:32633500</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ymu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[IGPS 3D structures\|IGPS 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Thermotoga maritima]]
		+	[[Category: Andrea K]]
		+	[[Category: Rajendran C]]
		+	[[Category: Sterner R]]

OCA at 10:57, 22 July 2020

OCA — Wed, 22 Jul 2020 10:57:09 GMT

←Older revision		Revision as of 10:57, 22 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ymu ~~is ON HOLD~~	+	====
-		+	<StructureSection load='6ymu' size='340' side='right'caption='[[6ymu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ymu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ymu OCA], [http://pdbe.org/6ymu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ymu RCSB], [http://www.ebi.ac.uk/pdbsum/6ymu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ymu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "6ymu" [edit=sysop:move=sysop]

OCA — Wed, 15 Apr 2020 05:38:52 GMT

Protected "6ymu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:38, 15 April 2020

OCA: New page: '''Unreleased structure''' The entry 6ymu is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Apr 2020 05:38:51 GMT

New page: '''Unreleased structure''' The entry 6ymu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ymu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6ymu - Revision history

OCA at 13:30, 24 January 2024

OCA at 10:57, 22 July 2020

OCA: Protected "6ymu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6ymu is ON HOLD Authors: Description: Category: Unreleased Structures