OCA at 13:32, 24 January 2024

OCA — Wed, 24 Jan 2024 13:32:57 GMT

←Older revision		Revision as of 13:32, 24 January 2024
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-	====	+	==14-3-3 sigma with RelA/p65 binding site pS45 and covalently bound TCF521-123==
-	<StructureSection load='6ypy' size='340' side='right'caption='[[6ypy]]' scene=''>	+	<StructureSection load='6ypy' size='340' side='right'caption='[[6ypy]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ypy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YPY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ypy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypy OCA], [~~http~~://pdbe.org/6ypy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ypy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ypy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=P7E:4-(4-ethanoylpiperazin-1-yl)sulfonylbenzaldehyde'>P7E</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypy OCA], [https://pdbe.org/6ypy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypy RCSB], [https://www.ebi.ac.uk/pdbsum/6ypy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Small-molecule stabilization of protein-protein interactions (PPI) is a promising concept in drug discovery, however the question how to identify or design chemical starting points in a 'bottom-up' approach is largely unanswered. Here we report a novel concept for identifying initial chemical matter for PPI stabilization based on covalent imine forming fragments. The imine bond offers a covalent anchor for site-directed fragment targeting, whereas its transient nature allows an efficient structure activity relationship analysis. This bond enables fragment identification and optimisation using protein crystallography. We report novel fragments which bind specifically to a lysine at the PPI interface of the p65 subunit-derived peptide of NF-kappaB with the adapter protein 14-3-3. Those fragments that subsequently establish contacts with the p65-derived peptide, rather than with 14-3-3, efficiently stabilize the 14-3-3/p65 complex and offer novel starting points for molecular glues.
		+
		+	Fragment Based Protein-Protein Interaction Stabilizers via Imine-Based Tethering.,Ottmann C, Wolter M, Valenti D, Cossar PJ, Levy LM, Hristeva S, Genski T, Hoffmann T, Brunsveld L, Tzalis D Angew Chem Int Ed Engl. 2020 Aug 20. doi: 10.1002/anie.202008585. PMID:32816380<ref>PMID:32816380</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ypy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Ottmann C]]
		+	[[Category: Wolter M]]

OCA at 11:14, 23 September 2020

OCA — Wed, 23 Sep 2020 11:14:24 GMT

←Older revision		Revision as of 11:14, 23 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ypy ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6ypy' size='340' side='right'caption='[[6ypy]]' scene=''>
-	~~Authors~~: ~~Wolter, M~~.~~, Ottmann, C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~14-3-3 sigma with RelA~~/~~p65 binding site pS45 and covalently bound TCF521~~-~~123~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ypy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypy OCA], [http://pdbe.org/6ypy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ypy RCSB], [http://www.ebi.ac.uk/pdbsum/6ypy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypy ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Ottmann, C~~]]	+	__TOC__
-	[[Category: ~~Wolter, M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "6ypy" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 04:32:27 GMT

Protected "6ypy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:32, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 6ypy is ON HOLD until Paper Publication Authors: Wolter, M., Ottmann, C. Description: 14-3-3 sigma with RelA/p65 binding site pS45 and covalently b...

OCA — Wed, 22 Apr 2020 04:32:26 GMT

New page: '''Unreleased structure''' The entry 6ypy is ON HOLD until Paper Publication Authors: Wolter, M., Ottmann, C. Description: 14-3-3 sigma with RelA/p65 binding site pS45 and covalently b...

New page

'''Unreleased structure'''

The entry 6ypy is ON HOLD until Paper Publication

Authors: Wolter, M., Ottmann, C.

Description: 14-3-3 sigma with RelA/p65 binding site pS45 and covalently bound TCF521-123
[[Category: Unreleased Structures]]
[[Category: Ottmann, C]]
[[Category: Wolter, M]]

6ypy - Revision history

OCA at 13:32, 24 January 2024

OCA at 11:14, 23 September 2020

OCA: Protected "6ypy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6ypy is ON HOLD until Paper Publication Authors: Wolter, M., Ottmann, C. Description: 14-3-3 sigma with RelA/p65 binding site pS45 and covalently b...