6yq6 - Revision history

OCA at 13:33, 24 January 2024

OCA — Wed, 24 Jan 2024 13:33:09 GMT

←Older revision		Revision as of 13:33, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6yq6' size='340' side='right'caption='[[6yq6]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='6yq6' size='340' side='right'caption='[[6yq6]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6yq6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_ql37~~ Streptomyces sp. ~~ql37~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YQ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6YQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._QL37 Streptomyces sp. QL37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YQ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C5F59_12925 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2093747 Streptomyces sp. QL37])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6yq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yq6 OCA], [~~http~~://pdbe.org/6yq6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6yq6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6yq6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6yq6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yq6 OCA], [https://pdbe.org/6yq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yq6 RCSB], [https://www.ebi.ac.uk/pdbsum/6yq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yq6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2S6PN47_9ACTN A0A2S6PN47_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~ql37~~]]	+	[[Category: Streptomyces sp. QL37]]
-	[[Category: Abrahams~~, J P~~]]	+	[[Category: Abrahams JP]]
-	[[Category: Elsayed~~, S S~~]]	+	[[Category: Elsayed SS]]
-	[[Category: Guo, R]]	+	[[Category: Guo R]]
-	~~[[Category: Heul, H van der~~]]	+	[[Category: Huang J]]
-	[[Category: Huang, J]]	+	[[Category: Prota A]]
-	[[Category: Prota, A]]	+	[[Category: Wu C]]
-	~~[[Category: Wezel, G P.van~~]]	+	[[Category: Xiao X]]
-	[[Category: Wu, C]]	+	[[Category: Van Wezel GP]]
-	[[Category: Xiao, X]]	+	[[Category: Van der Heul H]]
-	[[Category: ~~Antibiotic~~]]	+
-	[[Category: ~~Keto-reduction]]~~	+
-	~~[[Category: Lugdunomycin]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Short chain alcohol reductase~~]]	+

OCA at 07:51, 30 September 2020

OCA — Wed, 30 Sep 2020 07:51:55 GMT

←Older revision		Revision as of 07:51, 30 September 2020
Line 1:		Line 1:

	==Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis==		==Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis==
-	<StructureSection load='6yq6' size='340' side='right'caption='[[6yq6]]' scene=''>	+	<StructureSection load='6yq6' size='340' side='right'caption='[[6yq6]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yq6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._ql37 Streptomyces sp. ql37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YQ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yq6 OCA], [http://pdbe.org/6yq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6yq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yq6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C5F59_12925 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2093747 Streptomyces sp. QL37])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yq6 OCA], [http://pdbe.org/6yq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6yq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yq6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Angucyclines are a structurally diverse class of actinobacterial natural products defined by their varied polycyclic ring systems, which display a wide range of biological activities. We recently discovered lugdunomycin (1), a highly rearranged polyketide antibiotic derived from the angucycline backbone that is synthesized via several yet unexplained enzymatic reactions. Here, we show via in vivo, in vitro, and structural analysis that the promiscuous reductase LugOII catalyzes both a C6 and an unprecedented C1 ketoreduction. This then sets the stage for the subsequent C-ring cleavage that is key to the rearranged scaffolds of 1. The 1.1 A structures of LugOII in complex with either ligand 8-O-Methylrabelomycin (4) or 8-O-Methyltetrangomycin (5) and of apoenzyme were resolved, which revealed a canonical Rossman fold and a remarkable conformational change during substrate capture and release. Mutational analysis uncovered key residues for substrate access, position, and catalysis as well as specific determinants that control its dual functionality. The insights obtained in this work hold promise for the discovery and engineering of other promiscuous reductases that may be harnessed for the generation of novel biocatalysts for chemoenzymatic applications.
		+
		+	Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.,Xiao X, Elsayed SS, Wu C, van der Heul HU, Metsa-Ketela M, Du C, Prota AE, Chen CC, Liu W, Guo RT, Abrahams JP, van Wezel GP ACS Chem Biol. 2020 Sep 8. doi: 10.1021/acschembio.0c00564. PMID:32840360<ref>PMID:32840360</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yq6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abrahams JP]]	+	[[Category: Streptomyces sp. ql37]]
-	[[Category: Elsayed SS]]	+	[[Category: Abrahams, J P]]
-	[[Category: Guo R]]	+	[[Category: Elsayed, S S]]
-	[[Category: Huang J]]	+	[[Category: Guo, R]]
-	[[Category: Prota A]]	+	[[Category: Heul, H van der]]
-	[[Category: Wu C]]	+	[[Category: Huang, J]]
-	[[Category: Xiao X]]	+	[[Category: Prota, A]]
-	[[Category: ~~Van Wezel GP~~]]	+	[[Category: Wezel, G P.van]]
-	[[Category: ~~Van der Heul H~~]]	+	[[Category: Wu, C]]
		+	[[Category: Xiao, X]]
		+	[[Category: Antibiotic]]
		+	[[Category: Keto-reduction]]
		+	[[Category: Lugdunomycin]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Short chain alcohol reductase]]

OCA at 09:51, 16 September 2020

OCA — Wed, 16 Sep 2020 09:51:09 GMT

←Older revision		Revision as of 09:51, 16 September 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6yq6 ~~is ON HOLD until Paper Publication~~	+	==Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis==
-		+	<StructureSection load='6yq6' size='340' side='right'caption='[[6yq6]]' scene=''>
-	~~Authors~~: ~~Xiao, X~~.~~, Elsayed, S~~.S.~~, Wu, C~~.~~, van der Heul, H~~., ~~Prota, A~~., ~~Huang, J~~., ~~Guo, R~~., ~~Abrahams, J~~.P., ~~van Wezel, G~~.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YQ6 FirstGlance]. <br>
-	~~Description: Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yq6 OCA], [http://pdbe.org/6yq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yq6 RCSB], [http://www.ebi.ac.uk/pdbsum/6yq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yq6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Van Wezel, G.P~~]]	+	__TOC__
-	[[Category: ~~Abrahams, J.P~~]]	+	</StructureSection>
-	[[Category: Guo, R]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wu, C~~]]	+	[[Category: Abrahams JP]]
-	[[Category: ~~Van Der Heul, H~~]]	+	[[Category: Elsayed SS]]
-	[[Category: ~~Elsayed, S.S~~]]	+	[[Category: Guo R]]
-	[[Category: ~~Huang, J~~]]	+	[[Category: Huang J]]
-	[[Category: ~~Prota, A~~]]	+	[[Category: Prota A]]
-	[[Category: ~~Xiao, X~~]]	+	[[Category: Wu C]]
		+	[[Category: Xiao X]]
		+	[[Category: Van Wezel GP]]
		+	[[Category: Van der Heul H]]

OCA at 07:32, 22 July 2020

OCA — Wed, 22 Jul 2020 07:32:01 GMT

←Older revision		Revision as of 07:32, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yq6 is ON HOLD	+	The entry 6yq6 is ON HOLD until Paper Publication

	Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., van Wezel, G.P.		Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., van Wezel, G.P.

OCA at 05:17, 3 June 2020

OCA — Wed, 03 Jun 2020 05:17:20 GMT

←Older revision		Revision as of 05:17, 3 June 2020
Line 3:		Line 3:
	The entry 6yq6 is ON HOLD		The entry 6yq6 is ON HOLD

-	Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., Wezel, G.P.	+	Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., van Wezel, G.P.

	Description: Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis		Description: Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Elsayed, S.S]]~~	+	[[Category: Van Wezel, G.P]]
-	~~[[Category: Huang, J]]~~	+
-	~~[[Category:~~ Wezel, G.P]]	+
-	~~[[Category: Wu, C]]~~	+
-	~~[[Category: Prota, A~~]]	+
	[[Category: Abrahams, J.P]]		[[Category: Abrahams, J.P]]
	[[Category: Guo, R]]		[[Category: Guo, R]]
		+	[[Category: Wu, C]]
	[[Category: Van Der Heul, H]]		[[Category: Van Der Heul, H]]
		+	[[Category: Elsayed, S.S]]
		+	[[Category: Huang, J]]
		+	[[Category: Prota, A]]
	[[Category: Xiao, X]]		[[Category: Xiao, X]]

OCA: Protected "6yq6" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 04:33:18 GMT

Protected "6yq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:33, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 6yq6 is ON HOLD Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., Wezel, G.P. Description:...

OCA — Wed, 22 Apr 2020 04:33:16 GMT

New page: '''Unreleased structure''' The entry 6yq6 is ON HOLD Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., Wezel, G.P. Description:...

New page

'''Unreleased structure'''

The entry 6yq6 is ON HOLD

Authors: Xiao, X., Elsayed, S.S., Wu, C., van der Heul, H., Prota, A., Huang, J., Guo, R., Abrahams, J.P., Wezel, G.P.

Description: Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis
[[Category: Unreleased Structures]]
[[Category: Elsayed, S.S]]
[[Category: Huang, J]]
[[Category: Wezel, G.P]]
[[Category: Wu, C]]
[[Category: Prota, A]]
[[Category: Abrahams, J.P]]
[[Category: Guo, R]]
[[Category: Van Der Heul, H]]
[[Category: Xiao, X]]