6ytb - Revision history

OCA at 13:35, 24 January 2024

OCA — Wed, 24 Jan 2024 13:35:05 GMT

←Older revision		Revision as of 13:35, 24 January 2024
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-	====	+	==GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT==
-	<StructureSection load='6ytb' size='340' side='right'caption='[[6ytb]]' scene=''>	+	<StructureSection load='6ytb' size='340' side='right'caption='[[6ytb]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ytb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YTB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ytb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytb OCA], [~~http~~://pdbe.org/6ytb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ytb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ytb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytb ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ytb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytb OCA], [https://pdbe.org/6ytb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ytb RCSB], [https://www.ebi.ac.uk/pdbsum/6ytb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGG1_HUMAN IGG1_HUMAN] Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).<ref>PMID:17576170</ref> <ref>PMID:20176268</ref> <ref>PMID:22158414</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Generation of bispecific antibodies (bsAbs) requires a combination of compatible binders in formats that support desired functionalities. Here, we report that bsAb-matrices can be generated by Format Chain Exchange (FORCE), enabling screening of combinatorial binder/format spaces. Input molecules for generation of bi/multi-valent bsAbs are monospecific entities similar to knob-into-hole half-antibodies, yet with complementary CH3-interface-modulated and affinity-tagged dummy-chains. These contain mutations that lead to limited interface repulsions without compromising expression or biophysical properties of educts. Mild reduction of combinations of educts triggers spontaneous chain-exchange reactions driven by partially flawed CH3-educt interfaces resolving to perfect complementarity. This generates large bsAb matrices harboring different binders in multiple formats. Benign biophysical properties and good expression yields of educts, combined with simplicity of purification enables process automation. Examples that demonstrate the relevance of screening binder/format combinations are provided as a matrix of bsAbs that simultaneously bind Her1/Her2 and DR5 without encountering binder or format-inflicted interferences.
		+
		+	Format chain exchange (FORCE) for high-throughput generation of bispecific antibodies in combinatorial binder-format matrices.,Dengl S, Mayer K, Bormann F, Duerr H, Hoffmann E, Nussbaum B, Tischler M, Wagner M, Kuglstatter A, Leibrock L, Buldun C, Georges G, Brinkmann U Nat Commun. 2020 Oct 2;11(1):4974. doi: 10.1038/s41467-020-18477-7. PMID:33009381<ref>PMID:33009381</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ytb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Benz J]]
		+	[[Category: Kuglstatter A]]
		+	[[Category: Leibrock L]]

OCA at 05:50, 14 October 2020

OCA — Wed, 14 Oct 2020 05:50:20 GMT

←Older revision		Revision as of 05:50, 14 October 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ytb ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6ytb' size='340' side='right'caption='[[6ytb]]' scene=''>
-	~~Authors~~: ~~Kuglstatter, A~~.~~, Leibrock, L~~.~~, Benz, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: GLYCOSYLATED KNOB~~/~~DUMMY~~-~~HOLE~~/~~DUMMY FC FRAGMENT~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ytb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytb OCA], [http://pdbe.org/6ytb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ytb RCSB], [http://www.ebi.ac.uk/pdbsum/6ytb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytb ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Kuglstatter~~, A]]	+	__TOC__
-	[[Category: ~~Benz, J~~]]	+	</StructureSection>
-	[[Category: ~~Leibrock, L~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:34, 22 July 2020

OCA — Wed, 22 Jul 2020 07:34:48 GMT

←Older revision		Revision as of 07:34, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ytb is ON HOLD	+	The entry 6ytb is ON HOLD until Paper Publication

	Authors: Kuglstatter, A., Leibrock, L., Benz, J.		Authors: Kuglstatter, A., Leibrock, L., Benz, J.

OCA at 05:01, 27 May 2020

OCA — Wed, 27 May 2020 05:01:13 GMT

←Older revision		Revision as of 05:01, 27 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ytb is ON HOLD ~~until sometime in the future~~	+	The entry 6ytb is ON HOLD

	Authors: Kuglstatter, A., Leibrock, L., Benz, J.		Authors: Kuglstatter, A., Leibrock, L., Benz, J.

OCA at 04:38, 6 May 2020

OCA — Wed, 06 May 2020 04:38:47 GMT

←Older revision		Revision as of 04:38, 6 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ytb is ON HOLD	+	The entry 6ytb is ON HOLD until sometime in the future

	Authors: Kuglstatter, A., Leibrock, L., Benz, J.		Authors: Kuglstatter, A., Leibrock, L., Benz, J.
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	Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT		Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Kuglstatter, A]]
	[[Category: Benz, J]]		[[Category: Benz, J]]
	[[Category: Leibrock, L]]		[[Category: Leibrock, L]]
-	[[Category: Kuglstatter, A]]

OCA: Protected "6ytb" [edit=sysop:move=sysop]

OCA — Wed, 29 Apr 2020 05:32:45 GMT

Protected "6ytb" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 6ytb is ON HOLD Authors: Kuglstatter, A., Leibrock, L., Benz, J. Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT [[Category: Unreleased...

OCA — Wed, 29 Apr 2020 05:32:44 GMT

New page: '''Unreleased structure''' The entry 6ytb is ON HOLD Authors: Kuglstatter, A., Leibrock, L., Benz, J. Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 6ytb is ON HOLD

Authors: Kuglstatter, A., Leibrock, L., Benz, J.

Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT
[[Category: Unreleased Structures]]
[[Category: Benz, J]]
[[Category: Leibrock, L]]
[[Category: Kuglstatter, A]]