6yu9 - Revision history

OCA at 13:35, 24 January 2024

OCA — Wed, 24 Jan 2024 13:35:36 GMT

←Older revision		Revision as of 13:35, 24 January 2024
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	<StructureSection load='6yu9' size='340' side='right'caption='[[6yu9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6yu9' size='340' side='right'caption='[[6yu9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6yu9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Clostridium_autoethanogenum_dsm_10061~~ Clostridium autoethanogenum ~~dsm~~ 10061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6YU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yu9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_autoethanogenum_DSM_10061 Clostridium autoethanogenum DSM 10061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YU9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=XCC:FE(4)-NI(1)-S(4)+CLUSTER'>XCC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.904Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6yu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu9 OCA], [~~http~~://pdbe.org/6yu9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6yu9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6yu9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu9 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=XCC:FE(4)-NI(1)-S(4)+CLUSTER'>XCC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu9 OCA], [https://pdbe.org/6yu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yu9 RCSB], [https://www.ebi.ac.uk/pdbsum/6yu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U5RTE2_9CLOT U5RTE2_9CLOT] [https://www.uniprot.org/uniprot/U5RSY1_9CLOT U5RSY1_9CLOT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6yu9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6yu9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carbon monoxide dehydrogenase 3D structures\|Carbon monoxide dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium autoethanogenum ~~dsm~~ 10061]]	+	[[Category: Clostridium autoethanogenum DSM 10061]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lemaire~~, O N~~]]	+	[[Category: Lemaire ON]]
-	[[Category: Wagner, T]]	+	[[Category: Wagner T]]
-	~~[[Category: Acetogenesis]]~~	+
-	~~[[Category: Acetogenic bacteria]]~~	+
-	~~[[Category: C1-metabolism]]~~	+
-	~~[[Category: Carbon monoxide]]~~	+
-	~~[[Category: Co-dehydrogenase/acetyl-coa synthase]]~~	+
-	~~[[Category: Gas channeling]]~~	+
-	~~[[Category: Metallo-containing protein]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Waste-gas conversion]]~~	+
-	~~[[Category: X-ray crystal structure~~]]	+

OCA at 08:14, 11 November 2020

OCA — Wed, 11 Nov 2020 08:14:46 GMT

←Older revision		Revision as of 08:14, 11 November 2020
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	==CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution==		==CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution==
-	<StructureSection load='6yu9' size='340' side='right'caption='[[6yu9]]' scene=''>	+	<StructureSection load='6yu9' size='340' side='right'caption='[[6yu9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yu9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_autoethanogenum_dsm_10061 Clostridium autoethanogenum dsm 10061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YU9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu9 OCA], [http://pdbe.org/6yu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yu9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=XCC:FE(4)-NI(1)-S(4)+CLUSTER'>XCC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu9 OCA], [http://pdbe.org/6yu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yu9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridium autoethanogenum, the bacterial model for biological conversion of waste gases into biofuels, grows under extreme carbon-monoxide (CO) concentrations. The strictly anaerobic bacterium derives its entire cellular energy and carbon from this poisonous gas, therefore requiring efficient molecular machineries for CO-conversion. Here, we structurally and biochemically characterized the key enzyme of the CO-converting metabolism: the CO-dehydrogenase/Acetyl-CoA synthase (CODH/ACS). We obtained crystal structures of natively isolated complexes from fructose-grown and CO-grown C. autoethanogenum cultures. Both contain the same isoforms and if the overall structure adopts the classic alpha2beta2 architecture, comparable to the model enzyme from Moorella thermoacetica, the ACS binds a different position on the CODH core. The structural characterization of a proteolyzed complex and the conservation of the binding interface in close homologs rejected the possibility of a crystallization artefact. Therefore, the internal CO-channeling system, critical to transfer CO generated at the C-cluster to the ACS active site, drastically differs in the complex from C. autoethanogenum. The 1.9-A structure of the CODH alone provides an accurate picture of the new CO-routes, leading to the ACS core and reaching the surface. Increased gas accessibility would allow the simultaneous CO-oxidation and acetyl-CoA production. Biochemical experiments showed higher flexibility of the ACS subunit from C. autoethanogenum compared to M. thermoacetica, albeit monitoring similar CO-oxidation and formation rates. These results show a reshuffling of internal CO-tunnels during evolution of these Firmicutes, putatively leading to a bidirectional complex that ensure a high flux of CO-conversion toward energy conservation, acting as the main cellular powerplant.
		+
		+	Gas channel rerouting in a primordial enzyme: Structural insights of the carbon-monoxide dehydrogenase/acetyl-CoA synthase complex from the acetogen Clostridium autoethanogenum.,Lemaire ON, Wagner T Biochim Biophys Acta Bioenerg. 2020 Oct 17;1862(1):148330. doi:, 10.1016/j.bbabio.2020.148330. PMID:33080205<ref>PMID:33080205</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yu9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium autoethanogenum dsm 10061]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lemaire ON]]	+	[[Category: Lemaire, O N]]
-	[[Category: Wagner T]]	+	[[Category: Wagner, T]]
		+	[[Category: Acetogenesis]]
		+	[[Category: Acetogenic bacteria]]
		+	[[Category: C1-metabolism]]
		+	[[Category: Carbon monoxide]]
		+	[[Category: Co-dehydrogenase/acetyl-coa synthase]]
		+	[[Category: Gas channeling]]
		+	[[Category: Metallo-containing protein]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Waste-gas conversion]]
		+	[[Category: X-ray crystal structure]]

OCA at 07:24, 4 November 2020

OCA — Wed, 04 Nov 2020 07:24:30 GMT

←Older revision		Revision as of 07:24, 4 November 2020
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-	'''Unreleased structure'''

-	~~The entry 6yu9 is ON HOLD until Paper Publication~~	+	==CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution==
-		+	<StructureSection load='6yu9' size='340' side='right'caption='[[6yu9]]' scene=''>
-	~~Authors: Wagner, T., Lemaire, O.N.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YU9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YU9 FirstGlance]. <br>
-	~~Description:~~ CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yu9 OCA], [http://pdbe.org/6yu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yu9 RCSB], [http://www.ebi.ac.uk/pdbsum/6yu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yu9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wagner, T~~]]	+	__TOC__
-	[[Category: ~~Lemaire, O.N~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lemaire ON]]
		+	[[Category: Wagner T]]

OCA at 18:48, 28 October 2020

OCA — Wed, 28 Oct 2020 18:48:36 GMT

←Older revision		Revision as of 18:48, 28 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yu9 is ON HOLD	+	The entry 6yu9 is ON HOLD until Paper Publication

	Authors: Wagner, T., Lemaire, O.N.		Authors: Wagner, T., Lemaire, O.N.

OCA at 04:20, 7 October 2020

OCA — Wed, 07 Oct 2020 04:20:12 GMT

←Older revision		Revision as of 04:20, 7 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yu9 is ON HOLD ~~until Paper Publication~~	+	The entry 6yu9 is ON HOLD

	Authors: Wagner, T., Lemaire, O.N.		Authors: Wagner, T., Lemaire, O.N.

OCA at 09:27, 29 July 2020

OCA — Wed, 29 Jul 2020 09:27:05 GMT

←Older revision		Revision as of 09:27, 29 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yu9 is ON HOLD	+	The entry 6yu9 is ON HOLD until Paper Publication

	Authors: Wagner, T., Lemaire, O.N.		Authors: Wagner, T., Lemaire, O.N.

OCA: Protected "6yu9" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2020 04:40:45 GMT

Protected "6yu9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 6 May 2020

OCA: New page: '''Unreleased structure''' The entry 6yu9 is ON HOLD Authors: Wagner, T., Lemaire, O.N. Description: CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution [[...

OCA — Wed, 06 May 2020 04:40:44 GMT

New page: '''Unreleased structure''' The entry 6yu9 is ON HOLD Authors: Wagner, T., Lemaire, O.N. Description: CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution [[...

New page

'''Unreleased structure'''

The entry 6yu9 is ON HOLD

Authors: Wagner, T., Lemaire, O.N.

Description: CO-dehydrogenase homodimer from Clostridium autoethanogenum at 1.90-A resolution
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Lemaire, O.N]]