6yus - Revision history

OCA at 13:35, 24 January 2024

OCA — Wed, 24 Jan 2024 13:35:55 GMT

←Older revision		Revision as of 13:35, 24 January 2024
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	<StructureSection load='6yus' size='340' side='right'caption='[[6yus]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6yus' size='340' side='right'caption='[[6yus]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6yus]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Neimd Neimd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6YUS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yus]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_A Neisseria meningitidis serogroup A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YUS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~OAS~~:~~O-ACETYLSERINE~~'>~~OAS~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=OAS:O-ACETYLSERINE'>OAS</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">sacC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65699 NEIMD])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yus OCA], [https://pdbe.org/6yus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yus RCSB], [https://www.ebi.ac.uk/pdbsum/6yus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yus ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6yus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yus OCA], [~~http~~://pdbe.org/6yus PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6yus RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6yus PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6yus ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O68216_NEIMD O68216_NEIMD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Neimd~~]]	+	[[Category: Neisseria meningitidis serogroup A]]
-	[[Category: Cramer~~, J T~~]]	+	[[Category: Cramer JT]]
-	[[Category: Fedorov, R]]	+	[[Category: Fedorov R]]
-	[[Category: Fiebig, T]]	+	[[Category: Fiebig T]]
-	[[Category: Muehlenhoff, M]]	+	[[Category: Muehlenhoff M]]
-	~~[[Category: A/b hydrolase fold]]~~	+
-	~~[[Category: Catalytic triad]]~~	+
-	~~[[Category: O-acetyltransferase]]~~	+
-	~~[[Category: Serine transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:18, 14 October 2020

OCA — Wed, 14 Oct 2020 06:18:24 GMT

←Older revision		Revision as of 06:18, 14 October 2020
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	==Capsule O-acetyltransferase of Neisseria meningitidis serogroup A H228A mutant in complex with CoA==		==Capsule O-acetyltransferase of Neisseria meningitidis serogroup A H228A mutant in complex with CoA==
-	<StructureSection load='6yus' size='340' side='right'caption='[[6yus]]' scene=''>	+	<StructureSection load='6yus' size='340' side='right'caption='[[6yus]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yus]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimd Neimd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yus OCA], [http://pdbe.org/6yus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yus RCSB], [http://www.ebi.ac.uk/pdbsum/6yus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yus ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAS:O-ACETYLSERINE'>OAS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sacC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65699 NEIMD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yus OCA], [http://pdbe.org/6yus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yus RCSB], [http://www.ebi.ac.uk/pdbsum/6yus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yus ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	O-Acetylation of the capsular polysaccharide (CPS) of Neisseria meningitidis serogroup A (NmA) is critical for the induction of functional immune responses, making this modification mandatory for CPS-based anti-NmA vaccines. Using comprehensive NMR studies, we demonstrate that O-acetylation stabilizes the labile anomeric phosphodiester-linkages of the NmA-CPS and occurs in position C3 and C4 of the N-acetylmannosamine units due to enzymatic transfer and non-enzymatic ester migration, respectively. To shed light on the enzymatic transfer mechanism, we solved the crystal structure of the capsule O-acetyltransferase CsaC in its apo and acceptor-bound form and of the CsaC-H228A mutant as trapped acetyl-enzyme adduct in complex with CoA. Together with the results of a comprehensive mutagenesis study, the reported structures explain the strict regioselectivity of CsaC and provide insight into the catalytic mechanism, which relies on an unexpected Gln-extension of a classical Ser-His-Asp triad, embedded in an alpha/beta-hydrolase fold.
		+
		+	Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A.,Fiebig T, Cramer JT, Bethe A, Baruch P, Curth U, Fuhring JI, Buettner FFR, Vogel U, Schubert M, Fedorov R, Muhlenhoff M Nat Commun. 2020 Sep 18;11(1):4723. doi: 10.1038/s41467-020-18464-y. PMID:32948778<ref>PMID:32948778</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yus" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cramer JT]]	+	[[Category: Neimd]]
-	[[Category: Fedorov R]]	+	[[Category: Cramer, J T]]
-	[[Category: Fiebig T]]	+	[[Category: Fedorov, R]]
-	[[Category: Muehlenhoff M]]	+	[[Category: Fiebig, T]]
		+	[[Category: Muehlenhoff, M]]
		+	[[Category: A/b hydrolase fold]]
		+	[[Category: Catalytic triad]]
		+	[[Category: O-acetyltransferase]]
		+	[[Category: Serine transferase]]
		+	[[Category: Transferase]]

OCA at 06:23, 19 August 2020

OCA — Wed, 19 Aug 2020 06:23:52 GMT

←Older revision		Revision as of 06:23, 19 August 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6yus is ~~ON HOLD until Paper Publication~~	+	==Capsule O-acetyltransferase of Neisseria meningitidis serogroup A H228A mutant in complex with CoA==
-		+	<StructureSection load='6yus' size='340' side='right'caption='[[6yus]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yus OCA], [http://pdbe.org/6yus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yus RCSB], [http://www.ebi.ac.uk/pdbsum/6yus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yus ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cramer JT]]
		+	[[Category: Fedorov R]]
		+	[[Category: Fiebig T]]
		+	[[Category: Muehlenhoff M]]

OCA at 08:47, 17 June 2020

OCA — Wed, 17 Jun 2020 08:47:37 GMT

←Older revision		Revision as of 08:47, 17 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yus is ON HOLD	+	The entry 6yus is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6yus" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2020 04:42:42 GMT

Protected "6yus" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:42, 6 May 2020

OCA: New page: '''Unreleased structure''' The entry 6yus is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 May 2020 04:42:41 GMT

New page: '''Unreleased structure''' The entry 6yus is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6yus is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]