6ywr - Revision history

OCA at 13:37, 24 January 2024

OCA — Wed, 24 Jan 2024 13:37:16 GMT

←Older revision		Revision as of 13:37, 24 January 2024
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	==Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)==		==Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)==
-	<StructureSection load='6ywr' size='340' side='right'caption='[[6ywr]]' scene=''>	+	<StructureSection load='6ywr' size='340' side='right'caption='[[6ywr]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YWR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ywr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aggregans_DSM_9485 Chloroflexus aggregans DSM 9485]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YWR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ywr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ywr OCA], [https://pdbe.org/6ywr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ywr RCSB], [https://www.ebi.ac.uk/pdbsum/6ywr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ywr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ywr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ywr OCA], [https://pdbe.org/6ywr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ywr RCSB], [https://www.ebi.ac.uk/pdbsum/6ywr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ywr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B8GAY9_CHLAD B8GAY9_CHLAD]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Light-oxygen-voltage (LOV) domains are widespread photosensory modules that can be used in fluorescence microscopy, optogenetics and controlled production of reactive oxygen species. All of the currently known LOV domains have absorption maxima in the range of ~440 to ~450 nm, and it is not clear whether they can be shifted significantly using mutations. Here, we have generated a panel of LOV domain variants by mutating the key chromophore-proximal glutamine aminoacid of a thermostable flavin based fluorescent protein CagFbFP (Gln148) to asparagine, aspartate, glutamate, histidine, lysine and arginine. Absorption spectra of all of the mutants are blue-shifted, with the maximal shift of 8 nm observed for the Q148H variant. While CagFbFP and its Q148N/D/E variants are not sensitive to pH, Q148H/K/R reveal a moderate red shift induced byacidic pH. To gain further insight, we determined high resolution crystal structures of all of the mutants studied at the resolutions from 1.07 A for Q148D to 1.63 A for Q148R. Whereas in some of the variants, the aminoacid 148 remains in the vicinity of the flavin, in Q148K, Q148R and partially Q148D, the C-terminus of the protein unlatches and the side chain of the residue 148 is reoriented away from the chromophore. Our results explain the absence of color shifts from replacing Gln148 with charged aminoacids and pave the way for rational design of color-shifted flavin based fluorescent proteins.
		+
		+	Insights into the mechanisms of light-oxygen-voltage domain color tuning from a set of high-resolution X-ray structures.,Remeeva A, Nazarenko VV, Kovalev K, Goncharov IM, Yudenko A, Astashkin R, Gordeliy V, Gushchin I Proteins. 2021 Mar 27. doi: 10.1002/prot.26078. PMID:33774867<ref>PMID:33774867</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ywr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chloroflexus aggregans DSM 9485]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gushchin I]]		[[Category: Gushchin I]]

OCA at 08:20, 21 April 2021

OCA — Wed, 21 Apr 2021 08:20:58 GMT

←Older revision		Revision as of 08:20, 21 April 2021
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-	'''Unreleased structure'''

-	~~The entry 6ywr is ON HOLD until Paper Publication~~	+	==Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)==
-		+	<StructureSection load='6ywr' size='340' side='right'caption='[[6ywr]]' scene=''>
-	~~Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YWR FirstGlance]. <br>
-	~~Description:~~ Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ywr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ywr OCA], [https://pdbe.org/6ywr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ywr RCSB], [https://www.ebi.ac.uk/pdbsum/6ywr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ywr ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Nazarenko~~, V]]	+	__TOC__
-	[[Category: Gushchin, I]]	+	</StructureSection>
-	[[Category: Kovalev, K]]	+	[[Category: Large Structures]]
-	[[Category: Remeeva, A]]	+	[[Category: Gushchin I]]
		+	[[Category: Kovalev K]]
		+	[[Category: Nazarenko V]]
		+	[[Category: Remeeva A]]

OCA at 07:37, 22 July 2020

OCA — Wed, 22 Jul 2020 07:37:37 GMT

←Older revision		Revision as of 07:37, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ywr is ON HOLD	+	The entry 6ywr is ON HOLD until Paper Publication

	Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I.		Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I.
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	Description: Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)		Description: Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Nazarenko, V]]
	[[Category: Gushchin, I]]		[[Category: Gushchin, I]]
	[[Category: Kovalev, K]]		[[Category: Kovalev, K]]
	[[Category: Remeeva, A]]		[[Category: Remeeva, A]]
-	[[Category: Nazarenko, V]]

OCA: Protected "6ywr" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2020 04:48:11 GMT

Protected "6ywr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 6 May 2020

OCA: New page: '''Unreleased structure''' The entry 6ywr is ON HOLD Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I. Description: Structure of Chloroflexus aggregans flavin based fluore...

OCA — Wed, 06 May 2020 04:48:10 GMT

New page: '''Unreleased structure''' The entry 6ywr is ON HOLD Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I. Description: Structure of Chloroflexus aggregans flavin based fluore...

New page

'''Unreleased structure'''

The entry 6ywr is ON HOLD

Authors: Remeeva, A., Nazarenko, V., Kovalev, K., Gushchin, I.

Description: Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant (space group C2)
[[Category: Unreleased Structures]]
[[Category: Gushchin, I]]
[[Category: Kovalev, K]]
[[Category: Remeeva, A]]
[[Category: Nazarenko, V]]