6yx5 - Revision history

OCA at 13:37, 24 January 2024

OCA — Wed, 24 Jan 2024 13:37:36 GMT

←Older revision		Revision as of 13:37, 24 January 2024
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-	====	+	==Structure of DrrA from Legionella pneumophilia in complex with human Rab8a==
-	<StructureSection load='6yx5' size='340' side='right'caption='[[6yx5]]' scene=''>	+	<StructureSection load='6yx5' size='340' side='right'caption='[[6yx5]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yx5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YX5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yx5 OCA], [https://pdbe.org/6yx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yx5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yx5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.14Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=LJN:[(2~{R},3~{S},4~{R},5~{R})-5-[4-(acetamidomethyl)-1,2,3-triazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+dihydrogen+phosphate'>LJN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yx5 OCA], [https://pdbe.org/6yx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yx5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yx5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Legionella pneumophila infects eukaryotic cells by forming a replicative organelle - the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and post-translational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP), and this process is referred to as AMPylation. Here, we use a chemical approach to stabilise low-affinity Rab:DrrA complexes in a site-specific manner to gain insight into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The crystal structure of the Rab:DrrA complex reveals a previously unknown non-conventional Rab-binding site (NC-RBS). Biochemical characterisation demonstrates allosteric stimulation of the AMPylation activity of DrrA via Rab binding to the NC-RBS. We speculate that allosteric control of DrrA could in principle prevent random and potentially cytotoxic AMPylation in the host, thereby perhaps ensuring efficient infection by Legionella.
		+
		+	Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1.,Du J, Wrisberg MV, Gulen B, Stahl M, Pett C, Hedberg C, Lang K, Schneider S, Itzen A Nat Commun. 2021 Jan 19;12(1):460. doi: 10.1038/s41467-020-20702-2. PMID:33469029<ref>PMID:33469029</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yx5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ras-related protein Rab 3D structures\|Ras-related protein Rab 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Legionella pneumophila]]
		+	[[Category: Du J]]
		+	[[Category: Itzen A]]
		+	[[Category: Lang K]]
		+	[[Category: Schneider S]]
		+	[[Category: Von Wrisberg MK]]

OCA at 11:17, 3 February 2021

OCA — Wed, 03 Feb 2021 11:17:03 GMT

←Older revision		Revision as of 11:17, 3 February 2021
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	<StructureSection load='6yx5' size='340' side='right'caption='[[6yx5]]' scene=''>		<StructureSection load='6yx5' size='340' side='right'caption='[[6yx5]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6yx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yx5 OCA], [~~http~~://pdbe.org/6yx5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6yx5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6yx5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6yx5 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yx5 OCA], [https://pdbe.org/6yx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yx5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yx5 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 10:34, 24 December 2020

OCA — Thu, 24 Dec 2020 10:34:01 GMT

←Older revision		Revision as of 10:34, 24 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6yx5 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6yx5' size='340' side='right'caption='[[6yx5]]' scene=''>
-	~~Authors~~: ~~Schneider, S~~.~~, Du, J~~.~~, von Wrisberg, M~~.K.~~, Lang, K~~.~~, Itzen, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of DrrA from Legionella pneumophilia in complex with human Rab8a~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yx5 OCA], [http://pdbe.org/6yx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yx5 RCSB], [http://www.ebi.ac.uk/pdbsum/6yx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yx5 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Schneider, S~~]]	+	__TOC__
-	[~~[Category~~: ~~Von Wrisberg~~, M.K]]	+	</StructureSection>
-	[~~[Category~~: ~~Itzen~~, A]]	+	[[Category: Large Structures]]
-	[[Category: ~~Du, J~~]]	+	[[Category: Z-disk]]
-	[[Category: ~~Lang, K~~]]	+

OCA at 05:04, 10 June 2020

OCA — Wed, 10 Jun 2020 05:04:57 GMT

←Older revision		Revision as of 05:04, 10 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yx5 is ON HOLD	+	The entry 6yx5 is ON HOLD until Paper Publication

	Authors: Schneider, S., Du, J., von Wrisberg, M.K., Lang, K., Itzen, A.		Authors: Schneider, S., Du, J., von Wrisberg, M.K., Lang, K., Itzen, A.
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	Description: Structure of DrrA from Legionella pneumophilia in complex with human Rab8a		Description: Structure of DrrA from Legionella pneumophilia in complex with human Rab8a
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Schneider, S]]
	[[Category: Von Wrisberg, M.K]]		[[Category: Von Wrisberg, M.K]]
	[[Category: Itzen, A]]		[[Category: Itzen, A]]
-	[[Category: Schneider, S]]
	[[Category: Du, J]]		[[Category: Du, J]]
	[[Category: Lang, K]]		[[Category: Lang, K]]

OCA: Protected "6yx5" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2020 04:49:43 GMT

Protected "6yx5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 6 May 2020

OCA: New page: '''Unreleased structure''' The entry 6yx5 is ON HOLD Authors: Schneider, S., Du, J., von Wrisberg, M.K., Lang, K., Itzen, A. Description: Structure of DrrA from Legionella pneumophilia...

OCA — Wed, 06 May 2020 04:49:42 GMT

New page: '''Unreleased structure''' The entry 6yx5 is ON HOLD Authors: Schneider, S., Du, J., von Wrisberg, M.K., Lang, K., Itzen, A. Description: Structure of DrrA from Legionella pneumophilia...

New page

'''Unreleased structure'''

The entry 6yx5 is ON HOLD

Authors: Schneider, S., Du, J., von Wrisberg, M.K., Lang, K., Itzen, A.

Description: Structure of DrrA from Legionella pneumophilia in complex with human Rab8a
[[Category: Unreleased Structures]]
[[Category: Von Wrisberg, M.K]]
[[Category: Itzen, A]]
[[Category: Schneider, S]]
[[Category: Du, J]]
[[Category: Lang, K]]