OCA at 13:40, 24 January 2024

OCA — Wed, 24 Jan 2024 13:40:25 GMT

←Older revision		Revision as of 13:40, 24 January 2024
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-	====	+	==Structure of Clr4 mutant - F256A/F310A/F427A bound to SAH==
-	<StructureSection load='6z2a' size='340' side='right'caption='[[6z2a]]' scene=''>	+	<StructureSection load='6z2a' size='340' side='right'caption='[[6z2a]], [[Resolution\|resolution]] 2.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z2a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z2A FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z2a OCA], [https://pdbe.org/6z2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z2a RCSB], [https://www.ebi.ac.uk/pdbsum/6z2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z2a ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.456Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z2a OCA], [https://pdbe.org/6z2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z2a RCSB], [https://www.ebi.ac.uk/pdbsum/6z2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z2a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CLR4_SCHPO CLR4_SCHPO] Histone methyltransferase. Catalytic component of the rik1-associated E3 ubiquitin ligase complex that shows ubiquitin ligase activity and is required for histone H3K9 methylation. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci.<ref>PMID:16024659</ref> <ref>PMID:8138176</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SUV39 class of methyltransferase enzymes deposits histone H3 lysine 9 di- and trimethylation (H3K9me2/3), the hallmark of constitutive heterochromatin. How these enzymes are regulated to mark specific genomic regions as heterochromatic is poorly understood. Clr4 is the sole H3K9me2/3 methyltransferase in the fission yeast Schizosaccharomyces pombe, and recent evidence suggests that ubiquitination of lysine 14 on histone H3 (H3K14ub) plays a key role in H3K9 methylation. However, the molecular mechanism of this regulation and its role in heterochromatin formation remain to be determined. Our structure-function approach shows that the H3K14ub substrate binds specifically and tightly to the catalytic domain of Clr4, and thereby stimulates the enzyme by over 250-fold. Mutations that disrupt this mechanism lead to a loss of H3K9me2/3 and abolish heterochromatin silencing similar to clr4 deletion. Comparison with mammalian SET domain proteins suggests that the Clr4 SET domain harbors a conserved sensor for H3K14ub, which mediates licensing of heterochromatin formation.
		+
		+	SUV39 SET domains mediate crosstalk of heterochromatic histone marks.,Stirpe A, Guidotti N, Northall SJ, Kilic S, Hainard A, Vadas O, Fierz B, Schalch T Elife. 2021 Sep 15;10:e62682. doi: 10.7554/eLife.62682. PMID:34524082<ref>PMID:34524082</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z2a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histone methyltransferase 3D structures\|Histone methyltransferase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Schizosaccharomyces pombe 972h-]]
		+	[[Category: Schalch T]]
		+	[[Category: Stirpe A]]

OCA at 09:16, 26 May 2021

OCA — Wed, 26 May 2021 09:16:14 GMT

←Older revision		Revision as of 09:16, 26 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6z2a ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6z2a' size='340' side='right'caption='[[6z2a]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z2a OCA], [https://pdbe.org/6z2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z2a RCSB], [https://www.ebi.ac.uk/pdbsum/6z2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z2a ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:41, 22 July 2020

OCA — Wed, 22 Jul 2020 07:41:50 GMT

←Older revision		Revision as of 07:41, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z2a is ON HOLD	+	The entry 6z2a is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6z2a" [edit=sysop:move=sysop]

OCA — Wed, 20 May 2020 04:35:12 GMT

Protected "6z2a" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 20 May 2020

OCA: New page: '''Unreleased structure''' The entry 6z2a is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 May 2020 04:35:11 GMT

New page: '''Unreleased structure''' The entry 6z2a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6z2a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6z2a - Revision history

OCA at 13:40, 24 January 2024

OCA at 09:16, 26 May 2021

OCA at 07:41, 22 July 2020

OCA: Protected "6z2a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6z2a is ON HOLD Authors: Description: Category: Unreleased Structures