6z5x - Revision history

OCA at 13:42, 24 January 2024

OCA — Wed, 24 Jan 2024 13:42:29 GMT

←Older revision		Revision as of 13:42, 24 January 2024
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	==The RSL - sulfonato-calix[8]arene complex, P213 form, acetate pH 4.8==		==The RSL - sulfonato-calix[8]arene complex, P213 form, acetate pH 4.8==
-	<StructureSection load='6z5x' size='340' side='right'caption='[[6z5x]]' scene=''>	+	<StructureSection load='6z5x' size='340' side='right'caption='[[6z5x]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z5X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z5x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z5X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z5x OCA], [https://pdbe.org/6z5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z5x RCSB], [https://www.ebi.ac.uk/pdbsum/6z5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z5x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.14Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=EVB:sulfonato-calix[8]arene'>EVB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z5x OCA], [https://pdbe.org/6z5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z5x RCSB], [https://www.ebi.ac.uk/pdbsum/6z5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z5x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Precisely defined protein aggregates, as exemplified by crystals, have applications in functional materials. Consequently, engineered protein assembly is a rapidly growing field. Anionic calix[n]arenes are useful scaffolds that can mold to cationic proteins and induce oligomerization and assembly. Here, we describe protein-calixarene composites obtained via cocrystallization of commercially available sulfonato-calix[8]arene (sclx8) with the symmetric and "neutral" protein RSL. Cocrystallization occurred across a wide range of conditions and protein charge states, from pH 2.2-9.5, resulting in three crystal forms. Cationization of the protein surface at pH approximately 4 drives calixarene complexation and yielded two types of porous frameworks with pore diameters >3 nm. Both types of framework provide evidence of protein encapsulation by the calixarene. Calixarene-masked proteins act as nodes within the frameworks, displaying octahedral-type coordination in one case. The other framework formed millimeter-scale crystals within hours, without the need for precipitants or specialized equipment. NMR experiments revealed macrocycle-modulated side chain pKa values and suggested a mechanism for pH-triggered assembly. The same low pH framework was generated at high pH with a permanently cationic arginine-enriched RSL variant. Finally, in addition to protein framework fabrication, sclx8 enables de novo structure determination.
		+
		+	Facile Fabrication of Protein-Macrocycle Frameworks.,Ramberg KO, Engilberge S, Skorek T, Crowley PB J Am Chem Soc. 2021 Feb 3;143(4):1896-1907. doi: 10.1021/jacs.0c10697. Epub 2021 , Jan 20. PMID:33470808<ref>PMID:33470808</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z5x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Ralstonia solanacearum]]
	[[Category: Crowley PB]]		[[Category: Crowley PB]]
	[[Category: Engilberge S]]		[[Category: Engilberge S]]
	[[Category: Ramberg K]]		[[Category: Ramberg K]]

OCA at 06:46, 7 April 2021

OCA — Wed, 07 Apr 2021 06:46:33 GMT

←Older revision		Revision as of 06:46, 7 April 2021
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-	'''Unreleased structure'''

-	~~The entry 6z5x is ON HOLD until Paper Publication~~	+	==The RSL - sulfonato-calix[8]arene complex, P213 form, acetate pH 4.8==
-		+	<StructureSection load='6z5x' size='340' side='right'caption='[[6z5x]]' scene=''>
-	~~Authors: Engilberge, S., Ramberg, K., Crowley, P.B.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z5X FirstGlance]. <br>
-	~~Description:~~ The RSL -sulfonato-calix[8]arene complex, P213 form, acetate pH 4.8	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z5x OCA], [https://pdbe.org/6z5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z5x RCSB], [https://www.ebi.ac.uk/pdbsum/6z5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z5x ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Crowley~~, P.B]]	+	__TOC__
-	[[Category: ~~Ramberg, K~~]]	+	</StructureSection>
-	[[Category: Engilberge, S]]	+	[[Category: Large Structures]]
		+	[[Category: Crowley PB]]
		+	[[Category: Engilberge S]]
		+	[[Category: Ramberg K]]

OCA at 07:45, 22 July 2020

OCA — Wed, 22 Jul 2020 07:45:59 GMT

←Older revision		Revision as of 07:45, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z5x is ON HOLD	+	The entry 6z5x is ON HOLD until Paper Publication

	Authors: Engilberge, S., Ramberg, K., Crowley, P.B.		Authors: Engilberge, S., Ramberg, K., Crowley, P.B.

OCA: Protected "6z5x" [edit=sysop:move=sysop]

OCA — Wed, 03 Jun 2020 05:21:20 GMT

Protected "6z5x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:21, 3 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z5x is ON HOLD Authors: Engilberge, S., Ramberg, K., Crowley, P.B. Description: The RSL -sulfonato-calix[8]arene complex, P213 form, acetate pH 4....

OCA — Wed, 03 Jun 2020 05:21:19 GMT

New page: '''Unreleased structure''' The entry 6z5x is ON HOLD Authors: Engilberge, S., Ramberg, K., Crowley, P.B. Description: The RSL -sulfonato-calix[8]arene complex, P213 form, acetate pH 4....

New page

'''Unreleased structure'''

The entry 6z5x is ON HOLD

Authors: Engilberge, S., Ramberg, K., Crowley, P.B.

Description: The RSL -sulfonato-calix[8]arene complex, P213 form, acetate pH 4.8
[[Category: Unreleased Structures]]
[[Category: Crowley, P.B]]
[[Category: Ramberg, K]]
[[Category: Engilberge, S]]