OCA at 10:04, 21 December 2022

OCA — Wed, 21 Dec 2022 10:04:56 GMT

←Older revision		Revision as of 10:04, 21 December 2022
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	==Crystal structure of the HK97 bacteriophage large terminase==		==Crystal structure of the HK97 bacteriophage large terminase==
-	<StructureSection load='6z6d' size='340' side='right'caption='[[6z6d]]' scene=''>	+	<StructureSection load='6z6d' size='340' side='right'caption='[[6z6d]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z6d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6D FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6d OCA], [https://pdbe.org/6z6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6d RCSB], [https://www.ebi.ac.uk/pdbsum/6z6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6d ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6d OCA], [https://pdbe.org/6z6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6d RCSB], [https://www.ebi.ac.uk/pdbsum/6z6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6d ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9MCT1_BPHK7 Q9MCT1_BPHK7]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively translocate viral genomic DNA into procapsids during assembly. Our current understanding of viral DNA packaging comes from three archetypal bacteriophage systems: cos, pac and phi29. Detailed mechanistic understanding exists for pac and phi29, but not for cos. Here, we reconstituted in vitro a cos packaging system based on bacteriophage HK97 and provided a detailed biochemical and structural description. We used a photobleaching-based, single-molecule assay to determine the stoichiometry of the DNA-translocating ATPase large terminase. Crystal structures of the large terminase and DNA-recruiting small terminase, a first for a biochemically defined cos system, reveal mechanistic similarities between cos and pac systems. At the same time, mutational and biochemical analyses indicate a new regulatory mechanism for ATPase multimerization and coordination in the HK97 system. This work therefore establishes a framework for studying the evolutionary relationships between ATP-dependent DNA translocation machineries in double-stranded DNA viruses.
		+
		+	Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system.,Fung HKH, Grimes S, Huet A, Duda RL, Chechik M, Gault J, Robinson CV, Hendrix RW, Jardine PJ, Conway JF, Baumann CG, Antson AA Nucleic Acids Res. 2022 Aug 26;50(15):8719-8732. doi: 10.1093/nar/gkac647. PMID:35947691<ref>PMID:35947691</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z6d" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Terminase 3D Structures\|Terminase 3D Structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia virus HK97]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Antson AA]]		[[Category: Antson AA]]

OCA at 12:10, 9 June 2021

OCA — Wed, 09 Jun 2021 12:10:01 GMT

←Older revision		Revision as of 12:10, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6z6d is ~~ON HOLD until May 28 2022~~	+	==Crystal structure of the HK97 bacteriophage large terminase==
-		+	<StructureSection load='6z6d' size='340' side='right'caption='[[6z6d]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6D FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6d OCA], [https://pdbe.org/6z6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6d RCSB], [https://www.ebi.ac.uk/pdbsum/6z6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6d ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Antson AA]]
		+	[[Category: Baumann CG]]
		+	[[Category: Chechik M]]
		+	[[Category: Fung HKH]]

OCA at 07:46, 22 July 2020

OCA — Wed, 22 Jul 2020 07:46:24 GMT

←Older revision		Revision as of 07:46, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z6d is ON HOLD	+	The entry 6z6d is ON HOLD until May 28 2022

	Authors:		Authors:

OCA: Protected "6z6d" [edit=sysop:move=sysop]

OCA — Wed, 03 Jun 2020 05:22:04 GMT

Protected "6z6d" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 3 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z6d is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Jun 2020 05:22:03 GMT

New page: '''Unreleased structure''' The entry 6z6d is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6z6d is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6z6d - Revision history

OCA at 10:04, 21 December 2022

OCA at 12:10, 9 June 2021

OCA at 07:46, 22 July 2020

OCA: Protected "6z6d" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6z6d is ON HOLD Authors: Description: Category: Unreleased Structures