6z6w - Revision history

OCA at 07:51, 21 September 2022

OCA — Wed, 21 Sep 2022 07:51:38 GMT

←Older revision		Revision as of 07:51, 21 September 2022
Line 1:		Line 1:

-	====	+	==Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8) from a mammalian expression system.==
-	<StructureSection load='6z6w' size='340' side='right'caption='[[6z6w]]' scene=''>	+	<StructureSection load='6z6w' size='340' side='right'caption='[[6z6w]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z6w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_poliovirus_3 Human poliovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6w OCA], [~~http~~://pdbe.org/6z6w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6z6w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6z6w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6w ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPH:SPHINGOSINE'>SPH</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6w OCA], [https://pdbe.org/6z6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6w RCSB], [https://www.ebi.ac.uk/pdbsum/6z6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/Q84895_9ENTO Q84895_9ENTO]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome, represent safe next-generation vaccines, however their production requires optimisation. Here we present an efficient mammalian expression strategy producing good yields of wild-type PV VLPs for all three serotypes and a thermostabilised variant for PV3. Whilst the wild-type VLPs were predominantly in the non-native C-antigenic form, the thermostabilised PV3 VLPs adopted the native D-antigenic conformation eliciting neutralising antibody titres equivalent to the current IPV and were indistinguishable from natural empty particles by cryo-electron microscopy with a similar stabilising lipidic pocket-factor in the VP1 beta-barrel. This factor may not be available in alternative expression systems, which may require synthetic pocket-binding factors. VLPs equivalent to these mammalian expressed thermostabilized particles, represent safer non-infectious vaccine candidates for the post-eradication era.
		+
		+	Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines.,Bahar MW, Porta C, Fox H, Macadam AJ, Fry EE, Stuart DI NPJ Vaccines. 2021 Jan 8;6(1):5. doi: 10.1038/s41541-020-00267-3. PMID:33420068<ref>PMID:33420068</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z6w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human poliovirus 3]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bahar MW]]
		+	[[Category: Fry EE]]
		+	[[Category: Porta C]]
		+	[[Category: Stuart DI]]

OCA at 06:45, 30 December 2020

OCA — Wed, 30 Dec 2020 06:45:48 GMT

←Older revision		Revision as of 06:45, 30 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6z6w ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6z6w' size='340' side='right'caption='[[6z6w]]' scene=''>
-	~~Authors~~: ~~Bahar, M~~.W.~~, Porta, C~~.~~, Fry, E~~.E.~~, Stuart, D~~.I.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8) from a mammalian expression system~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6w OCA], [http://pdbe.org/6z6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z6w RCSB], [http://www.ebi.ac.uk/pdbsum/6z6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6w ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[~~[Category~~: ~~Porta, C~~]]	+	__TOC__
-	[~~[Category~~: ~~Stuart, D~~.I]]	+	</StructureSection>
-	[[Category: ~~Fry, E.E~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bahar, M.W~~]]	+	[[Category: Z-disk]]

OCA at 08:48, 17 June 2020

OCA — Wed, 17 Jun 2020 08:48:46 GMT

←Older revision		Revision as of 08:48, 17 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z6w is ON HOLD	+	The entry 6z6w is ON HOLD until Paper Publication

	Authors: Bahar, M.W., Porta, C., Fry, E.E., Stuart, D.I.		Authors: Bahar, M.W., Porta, C., Fry, E.E., Stuart, D.I.

OCA: Protected "6z6w" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:06:47 GMT

Protected "6z6w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z6w is ON HOLD Authors: Bahar, M.W., Porta, C., Fry, E.E., Stuart, D.I. Description: Poliovirus type 3 (strain Saukett) stabilised virus-like part...

OCA — Wed, 10 Jun 2020 05:06:46 GMT

New page: '''Unreleased structure''' The entry 6z6w is ON HOLD Authors: Bahar, M.W., Porta, C., Fry, E.E., Stuart, D.I. Description: Poliovirus type 3 (strain Saukett) stabilised virus-like part...

New page

'''Unreleased structure'''

The entry 6z6w is ON HOLD

Authors: Bahar, M.W., Porta, C., Fry, E.E., Stuart, D.I.

Description: Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8) from a mammalian expression system.
[[Category: Unreleased Structures]]
[[Category: Porta, C]]
[[Category: Stuart, D.I]]
[[Category: Fry, E.E]]
[[Category: Bahar, M.W]]