6z7t - Revision history

OCA at 13:43, 24 January 2024

OCA — Wed, 24 Jan 2024 13:43:13 GMT

←Older revision		Revision as of 13:43, 24 January 2024
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	<StructureSection load='6z7t' size='340' side='right'caption='[[6z7t]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='6z7t' size='340' side='right'caption='[[6z7t]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6z7t]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dicdi Dicdi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6Z7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z7T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6z7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7t OCA], [~~http~~://pdbe.org/6z7t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6z7t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6z7t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7t OCA], [https://pdbe.org/6z7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z7t RCSB], [https://www.ebi.ac.uk/pdbsum/6z7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.	+	[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6z7t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6z7t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Myosin 3D Structures\|Myosin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicdi~~]]	+	[[Category: Dictyostelium discoideum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ewert, W]]	+	[[Category: Ewert W]]
-	[[Category: Preller, M]]	+	[[Category: Preller M]]
-	~~[[Category: Adp-release]]~~	+
-	~~[[Category: Blebbistatin]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Motor protein]]~~	+
-	~~[[Category: Motorprotein]]~~	+
-	~~[[Category: Myosin~~]]	+

OCA at 20:54, 28 October 2020

OCA — Wed, 28 Oct 2020 20:54:07 GMT

←Older revision		Revision as of 20:54, 28 October 2020
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	==Nucleotide-free Myosin-II motor domain==		==Nucleotide-free Myosin-II motor domain==
-	<StructureSection load='6z7t' size='340' side='right'caption='[[6z7t]]' scene=''>	+	<StructureSection load='6z7t' size='340' side='right'caption='[[6z7t]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z7T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7t OCA], [http://pdbe.org/6z7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z7t RCSB], [http://www.ebi.ac.uk/pdbsum/6z7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7t ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7t OCA], [http://pdbe.org/6z7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z7t RCSB], [http://www.ebi.ac.uk/pdbsum/6z7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The motor protein myosin drives a wide range of cellular and muscular functions by generating directed movement and force, fueled through adenosine triphosphate (ATP) hydrolysis. Release of the hydrolysis product adenosine diphosphate (ADP) is a fundamental and regulatory process during force production. However, details about the molecular mechanism accompanying ADP release are scarce due to the lack of representative structures. Here we solved a novel blebbistatin-bound myosin conformation with critical structural elements in positions between the myosin pre-power stroke and rigor states. ADP in this structure is repositioned towards the surface by the phosphate-sensing P-loop, and stabilized in a partially unbound conformation via a salt-bridge between Arg131 and Glu187. A 5 A rotation separates the mechanical converter in this conformation from the rigor position. The crystallized myosin structure thus resembles a conformation towards the end of the two-step power stroke, associated with ADP release. Computationally reconstructing ADP release from myosin by means of molecular dynamics simulations further supported the existence of an equivalent conformation along the power stroke that shows the same major characteristics in the myosin motor domain as the resolved blebbistatin-bound myosin-II.ADP crystal structure, and identified a communication hub centered on Arg232 that mediates chemomechanical energy transduction.
		+
		+	Structural and Computational Insights into a Blebbistatin-Bound Myosin*ADP Complex with Characteristics of an ADP-Release Conformation along the Two-Step Myosin Power Stoke.,Ewert W, Franz P, Tsiavaliaris G, Preller M Int J Mol Sci. 2020 Oct 8;21(19). pii: ijms21197417. doi: 10.3390/ijms21197417. PMID:33049993<ref>PMID:33049993</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z7t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dicdi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ewert W]]	+	[[Category: Ewert, W]]
-	[[Category: Preller M]]	+	[[Category: Preller, M]]
		+	[[Category: Adp-release]]
		+	[[Category: Blebbistatin]]
		+	[[Category: Complex]]
		+	[[Category: Hydrolase]]
		+	[[Category: Inhibitor]]
		+	[[Category: Motor protein]]
		+	[[Category: Motorprotein]]
		+	[[Category: Myosin]]

OCA at 08:15, 21 October 2020

OCA — Wed, 21 Oct 2020 08:15:35 GMT

←Older revision		Revision as of 08:15, 21 October 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6z7t ~~is ON HOLD until Paper Publication~~	+	==Nucleotide-free Myosin-II motor domain==
-		+	<StructureSection load='6z7t' size='340' side='right'caption='[[6z7t]]' scene=''>
-	~~Authors~~: ~~Ewert~~, W., ~~Preller~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z7T FirstGlance]. <br>
-	~~Description~~: ~~Nucleotide~~-~~free Myosin-II motor domain~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7t OCA], [http://pdbe.org/6z7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z7t RCSB], [http://www.ebi.ac.uk/pdbsum/6z7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7t ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Ewert, W]]	+	__TOC__
-	[[Category: Preller, M]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ewert W]]
		+	[[Category: Preller M]]

OCA at 07:47, 22 July 2020

OCA — Wed, 22 Jul 2020 07:47:19 GMT

←Older revision		Revision as of 07:47, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z7t is ON HOLD	+	The entry 6z7t is ON HOLD until Paper Publication

	Authors: Ewert, W., Preller, M.		Authors: Ewert, W., Preller, M.

OCA: Protected "6z7t" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:08:57 GMT

Protected "6z7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:08, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z7t is ON HOLD Authors: Ewert, W., Preller, M. Description: Nucleotide-free Myosin-II motor domain Category: Unreleased Structures [[Category:...

OCA — Wed, 10 Jun 2020 05:08:56 GMT

New page: '''Unreleased structure''' The entry 6z7t is ON HOLD Authors: Ewert, W., Preller, M. Description: Nucleotide-free Myosin-II motor domain Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 6z7t is ON HOLD

Authors: Ewert, W., Preller, M.

Description: Nucleotide-free Myosin-II motor domain
[[Category: Unreleased Structures]]
[[Category: Ewert, W]]
[[Category: Preller, M]]