6z7x - Revision history

OCA at 13:43, 24 January 2024

OCA — Wed, 24 Jan 2024 13:43:19 GMT

←Older revision		Revision as of 13:43, 24 January 2024
Line 1:		Line 1:

-	====	+	==Insulin analytical antibody OXI-005 Fab==
-	<StructureSection load='6z7x' size='340' side='right'caption='[[6z7x]]' scene=''>	+	<StructureSection load='6z7x' size='340' side='right'caption='[[6z7x]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z7x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7x OCA], [https://pdbe.org/6z7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z7x RCSB], [https://www.ebi.ac.uk/pdbsum/6z7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7x OCA], [https://pdbe.org/6z7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z7x RCSB], [https://www.ebi.ac.uk/pdbsum/6z7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The insulin epitopes for two monoclonal antibodies (mAbs), OXI-005 and HUI-018, commonly used in combination for insulin concentration determination in sandwich assays, were determined using X-ray crystallography. The crystal structure of the HUI-018 Fab in complex with human insulin (HI) was determined and OXI-005 Fab crystal structures were determined in complex with HI and porcine insulin (PI) as well as on its own. The OXI-005 epitope comprises insulin residues 1,3,4,19-21 (A-chain) and 25-30 (B-chain) and for HUI-018 residues 7,8,10-14,17 (A-chain) and 5-7, 10, 14 (B-chain). The areas of insulin involved in interactions with the mAb are 20% (OXI-005) and 24% (HUI-018) of the total insulin surface. Based on the Fab complex crystal structures with the insulins a molecular model for simultaneous binding of the Fabs to PI was built and this model was validated by small angle X-ray scattering measurements for the ternary complex. The epitopes for the mAbs on insulin were found well separated from each other as expected from luminiscent oxygen channeling immunoassay results for different insulins (HI, PI, bovine insulin, DesB30 HI, insulin glargine, insulin lispro). The affinities of the OXI-005 and HUI-018 Fabs for HI, PI, and DesB30 HI were determined using surface plasmon resonance. The KD s were found to be in the range of 1-4 nM for the HUI-018 Fab, while more different for the OXI-005 Fab (50 nM for HI, 20 nM for PI and 400 nM for DesB30 HI) supporting the importance of residue B30 for binding to OXI-005.
		+
		+	Insulin binding to the analytical antibody sandwich pair OXI-005 and HUI-018: Epitope mapping and binding properties.,Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen CB, Poulsen F Protein Sci. 2020 Dec 5. doi: 10.1002/pro.4009. PMID:33277949<ref>PMID:33277949</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z7x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Johansson E]]

OCA at 14:59, 3 March 2021

OCA — Wed, 03 Mar 2021 14:59:38 GMT

←Older revision		Revision as of 14:59, 3 March 2021
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	<StructureSection load='6z7x' size='340' side='right'caption='[[6z7x]]' scene=''>		<StructureSection load='6z7x' size='340' side='right'caption='[[6z7x]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7x OCA], [~~http~~://pdbe.org/6z7x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6z7x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6z7x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7x OCA], [https://pdbe.org/6z7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z7x RCSB], [https://www.ebi.ac.uk/pdbsum/6z7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7x ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 06:46, 30 December 2020

OCA — Wed, 30 Dec 2020 06:46:00 GMT

←Older revision		Revision as of 06:46, 30 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6z7x ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6z7x' size='340' side='right'caption='[[6z7x]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z7x OCA], [http://pdbe.org/6z7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z7x RCSB], [http://www.ebi.ac.uk/pdbsum/6z7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z7x ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:49, 17 June 2020

OCA — Wed, 17 Jun 2020 08:49:17 GMT

←Older revision		Revision as of 08:49, 17 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z7x is ON HOLD	+	The entry 6z7x is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6z7x" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:09:09 GMT

Protected "6z7x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:09, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z7x is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Jun 2020 05:09:08 GMT

New page: '''Unreleased structure''' The entry 6z7x is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6z7x is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]