6z8l - Revision history

OCA at 13:43, 24 January 2024

OCA — Wed, 24 Jan 2024 13:43:45 GMT

←Older revision		Revision as of 13:43, 24 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6z8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z8L FirstGlance]. <br>		<table><tr><td colspan='2'>[[6z8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z8L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4000037Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z8l OCA], [https://pdbe.org/6z8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z8l RCSB], [https://www.ebi.ac.uk/pdbsum/6z8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z8l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z8l OCA], [https://pdbe.org/6z8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z8l RCSB], [https://www.ebi.ac.uk/pdbsum/6z8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z8l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AMYP_HUMAN AMYP_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alpha-amylase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adam, S]]	+	[[Category: Adam S]]
-	[[Category: Koehnke, J]]	+	[[Category: Koehnke J]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Maltose]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 10:09, 7 July 2021

OCA — Wed, 07 Jul 2021 10:09:35 GMT

←Older revision		Revision as of 10:09, 7 July 2021
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	==Alpha-Amylase in complex with probe fragments==		==Alpha-Amylase in complex with probe fragments==
-	<StructureSection load='6z8l' size='340' side='right'caption='[[6z8l]]' scene=''>	+	<StructureSection load='6z8l' size='340' side='right'caption='[[6z8l]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z8L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6Z8L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z8L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6z8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z8l OCA], [~~http~~://pdbe.org/6z8l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6z8l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6z8l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6z8l ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z8l OCA], [https://pdbe.org/6z8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z8l RCSB], [https://www.ebi.ac.uk/pdbsum/6z8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z8l ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Single site OH --> F substitution at the termini of maltotetraose leads to significantly improved hydrolytic stability towards alpha-amylase and alpha-glucosidase relative to the natural compound. To explore the effect of molecular editing, selectively modified oligosaccharides were prepared via a convergent alpha-selective strategy. Incubation experiments in purified alpha-amylase and alpha-glucosidase, and in human and murine blood serum, provide insight into the influence of fluorine on the hydrolytic stability of these clinically important scaffolds. Enhancements of ca. 1 order of magnitude result from these subtle single point mutations. Modification at the monosaccharide furthest from the probable enzymatic cleavage termini leads to the greatest improvement in stability. In the case of alpha-amylase, docking studies revealed that retentive C2-fluorination at the reducing end inverts the orientation in which the substrate is bound. A co-crystal structure of human alpha-amylase revealed maltose units bound at the active-site. In view of the evolving popularity of C(sp(3))-F bioisosteres in medicinal chemistry, and the importance of maltodextrins in bacterial imaging, this discovery begins to reconcile the information-rich nature of carbohydrates with their intrinsic hydrolytic vulnerabilities.
		+
		+	Enhancing glycan stability via site-selective fluorination: modulating substrate orientation by molecular design.,Axer A, Jumde RP, Adam S, Faust A, Schafers M, Fobker M, Koehnke J, Hirsch AKH, Gilmour R Chem Sci. 2020 Nov 23;12(4):1286-1294. doi: 10.1039/d0sc04297h. PMID:34163891<ref>PMID:34163891</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z8l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Amylase 3D structures\|Amylase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alpha-amylase]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adam S]]	+	[[Category: Adam, S]]
-	[[Category: Koehnke J]]	+	[[Category: Koehnke, J]]
		+	[[Category: Complex]]
		+	[[Category: Maltose]]
		+	[[Category: Sugar binding protein]]

OCA at 07:48, 2 December 2020

OCA — Wed, 02 Dec 2020 07:48:38 GMT

←Older revision		Revision as of 07:48, 2 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6z8l ~~is ON HOLD~~	+	==Alpha-Amylase in complex with probe fragments==
-		+	<StructureSection load='6z8l' size='340' side='right'caption='[[6z8l]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z8L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z8L FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z8l OCA], [http://pdbe.org/6z8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z8l RCSB], [http://www.ebi.ac.uk/pdbsum/6z8l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z8l ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Adam S]]
		+	[[Category: Koehnke J]]

OCA: Protected "6z8l" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:10:43 GMT

Protected "6z8l" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:10, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z8l is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Jun 2020 05:10:42 GMT

New page: '''Unreleased structure''' The entry 6z8l is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6z8l is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]