6z92 - Revision history

OCA at 06:59, 30 December 2020

OCA — Wed, 30 Dec 2020 06:59:50 GMT

←Older revision		Revision as of 06:59, 30 December 2020
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	==[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing==		==[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing==
-	<StructureSection load='6z92' size='340' side='right'caption='[[6z92]]' scene=''>	+	<StructureSection load='6z92' size='340' side='right'caption='[[6z92]], [[Resolution\|resolution]] 2.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z92 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z92 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6z92]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z92 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z92 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z92 OCA], [http://pdbe.org/6z92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z92 RCSB], [http://www.ebi.ac.uk/pdbsum/6z92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z92 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z92 OCA], [http://pdbe.org/6z92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z92 RCSB], [http://www.ebi.ac.uk/pdbsum/6z92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z92 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We recently discovered a [Fe-S]-containing protein with in vivo thiouracil desulfidase activity called TudS. We report here the crystal structure of TudS, refined at 1.5 A resolution, which harbors a [4Fe-4S] cluster, bound by three cysteines only. Incubation of TudS crystals with 4-thiouracil trapped the cluster with a hydrosulfide ligand bound to the fourth non-protein-bonded iron, as established by the sulfur anomalous signal. This indicates that a [4Fe-5S] state of the cluster is a catalytic intermediate in the desulfuration reaction. Structural data and site-directed mutagenesis indicate that a water molecule is located next to the hydrosulfide ligand and to two catalytically important residues, Ser101 and Glu45. This information together with modeling studies allow us to propose a mechanism for the unprecedented nonredox enzymatic desulfuration of thiouracil, in which a [4Fe-4S] cluster binds and activates the sulfur atom of the substrate.
		+
		+	Structural evidence for a [4Fe-5S] intermediate in the non-redox desulfuration of thiouracil.,Zhou J, Pecqueur L, Aucynaite A, Fuchs J, Rutkiene R, Vaitekunas J, Meskys R, Boll M, Fontecave M, Urbonavicius J, Golinelli-Pimpaneau B Angew Chem Int Ed Engl. 2020 Sep 15. doi: 10.1002/anie.202011211. PMID:32929873<ref>PMID:32929873</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6z92" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fontecave M]]	+	[[Category: Uncultivated bacterium]]
-	[[Category: Golinelli-Pimpaneau B]]	+	[[Category: Fontecave, M]]
-	[[Category: Pecqueur L]]	+	[[Category: Golinelli-Pimpaneau, B]]
-	[[Category: Zhou J]]	+	[[Category: Pecqueur, L]]
		+	[[Category: Zhou, J]]
		+	[[Category: Desulfuration]]
		+	[[Category: Iron-sulfur cluster]]
		+	[[Category: Sulfurtransferase]]
		+	[[Category: Thiouracil]]
		+	[[Category: Transferase]]

OCA at 07:12, 30 September 2020

OCA — Wed, 30 Sep 2020 07:12:02 GMT

←Older revision		Revision as of 07:12, 30 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6z92 is ~~ON HOLD until Paper Publication~~	+	==[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing==
-		+	<StructureSection load='6z92' size='340' side='right'caption='[[6z92]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z92 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z92 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z92 OCA], [http://pdbe.org/6z92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z92 RCSB], [http://www.ebi.ac.uk/pdbsum/6z92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z92 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Fontecave M]]
		+	[[Category: Golinelli-Pimpaneau B]]
		+	[[Category: Pecqueur L]]
		+	[[Category: Zhou J]]

OCA at 07:49, 22 July 2020

OCA — Wed, 22 Jul 2020 07:49:23 GMT

←Older revision		Revision as of 07:49, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6z92 is ON HOLD	+	The entry 6z92 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6z92" [edit=sysop:move=sysop]

OCA — Wed, 10 Jun 2020 05:11:33 GMT

Protected "6z92" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 10 June 2020

OCA: New page: '''Unreleased structure''' The entry 6z92 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Jun 2020 05:11:32 GMT

New page: '''Unreleased structure''' The entry 6z92 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6z92 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]