6zb6 - Revision history

OCA at 13:45, 24 January 2024

OCA — Wed, 24 Jan 2024 13:45:50 GMT

←Older revision		Revision as of 13:45, 24 January 2024
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	<StructureSection load='6zb6' size='340' side='right'caption='[[6zb6]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6zb6' size='340' side='right'caption='[[6zb6]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6zb6]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Annual_ryegrass Annual ryegrass~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZB6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ZB6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zb6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lolium_rigidum Lolium rigidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZB6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=GTB:S-(P-NITROBENZYL)GLUTATHIONE'>GTB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=89674 Annual ryegrass])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=GTB:S-(P-NITROBENZYL)GLUTATHIONE'>GTB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zb6 OCA], [~~http~~://pdbe.org/6zb6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zb6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zb6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zb6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zb6 OCA], [https://pdbe.org/6zb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zb6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zb6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M5BPX4_LOLRI M5BPX4_LOLRI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6zb6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6zb6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Annual ryegrass]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Papageorgiou, A C~~]]	+	[[Category: Lolium rigidum]]
-	[[Category: ~~Poudel, N~~]]	+	[[Category: Papageorgiou AC]]
-	[[Category: ~~Detoxification]]~~	+	[[Category: Poudel N]]
-	~~[[Category: Glutathione]]~~	+
-	~~[[Category: Herbcide resistance]]~~	+
-	~~[[Category: Stress]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Xenobiotic~~]]	+

OCA at 07:00, 30 December 2020

OCA — Wed, 30 Dec 2020 07:00:21 GMT

←Older revision		Revision as of 07:00, 30 December 2020
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	==Crystal structure of Lolium rigidum GSTF in complex with S-(p-nitrobenzyl) glutathione==		==Crystal structure of Lolium rigidum GSTF in complex with S-(p-nitrobenzyl) glutathione==
-	<StructureSection load='6zb6' size='340' side='right'caption='[[6zb6]]' scene=''>	+	<StructureSection load='6zb6' size='340' side='right'caption='[[6zb6]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZB6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZB6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zb6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Annual_ryegrass Annual ryegrass]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZB6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZB6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zb6 OCA], [http://pdbe.org/6zb6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zb6 RCSB], [http://www.ebi.ac.uk/pdbsum/6zb6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zb6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=GTB:S-(P-NITROBENZYL)GLUTATHIONE'>GTB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=89674 Annual ryegrass])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zb6 OCA], [http://pdbe.org/6zb6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zb6 RCSB], [http://www.ebi.ac.uk/pdbsum/6zb6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zb6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multiple-herbicide resistance (MHR) is a global threat to weed control in cereal crops. MHR weeds express a specific phi class glutathione transferase (MHR-GSTF) that confers resistance against multiple herbicides and therefore represents a promising target against MHR weeds. Kinetics inhibition analysis of MHR-GSTFs from grass weeds Lolium rigidum (LrGSTF) Alopecurus myosuroides (AmGSTF) and crops Hordeum vulgare (HvGSTF) and Triticum aestivum (TaGSTF) allowed the identification of the acetanilide herbicide butachlor as a potent and selective inhibitor towards MHR-GSTFs. Also, butachlor is a stronger inhibitor for LrGSTF and AmGSTF compared to HvGSTF and TaGSTF from crops. The crystal structure of LrGSTF was determined at 1.90 A resolution in complex with the inhibitor S-(4-nitrobenzyl)glutathione. A specific 3D pharmacophore targeting the MHR-GSTFs was designed and used to identify structural elements important for potent and selective inhibition. Structural analysis of GSTFs revealed a decisive role of conserved Tyr118 in ligand binding and pharmacophore design. Its positioning is dependent on an outer patch of adjacent residues that span from position 132 to 134 which are similar for both LrGSTF and AmGSTF but different in HvGSTF and TaGSTF. The results presented here provide new knowledge that may be adopted to cope with MHR weeds.
		+
		+	Phi class glutathione transferases as molecular targets towards multiple-herbicide resistance: Inhibition analysis and pharmacophore design.,Georgakis N, Poudel N, Vlachakis D, Papageorgiou AC, Labrou NE Plant Physiol Biochem. 2020 Nov 17. pii: S0981-9428(20)30569-6. doi:, 10.1016/j.plaphy.2020.11.018. PMID:33257232<ref>PMID:33257232</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zb6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Annual ryegrass]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Papageorgiou AC]]	+	[[Category: Papageorgiou, A C]]
-	[[Category: Poudel N]]	+	[[Category: Poudel, N]]
		+	[[Category: Detoxification]]
		+	[[Category: Glutathione]]
		+	[[Category: Herbcide resistance]]
		+	[[Category: Stress]]
		+	[[Category: Transferase]]
		+	[[Category: Xenobiotic]]

OCA at 06:38, 9 December 2020

OCA — Wed, 09 Dec 2020 06:38:30 GMT

←Older revision		Revision as of 06:38, 9 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6zb6 is ON HOLD until Paper Publication~~	+	==Crystal structure of Lolium rigidum GSTF in complex with S-(p-nitrobenzyl) glutathione==
-		+	<StructureSection load='6zb6' size='340' side='right'caption='[[6zb6]]' scene=''>
-	~~Authors: Papageorgiou, A.C., Poudel, N.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZB6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZB6 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of Lolium rigidum GSTF in complex with S-(p-nitrobenzyl) glutathione	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zb6 OCA], [http://pdbe.org/6zb6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zb6 RCSB], [http://www.ebi.ac.uk/pdbsum/6zb6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zb6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Papageorgiou~~, A.C~~]]	+	__TOC__
-	[[Category: Poudel, N]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Papageorgiou AC]]
		+	[[Category: Poudel N]]

OCA: Protected "6zb6" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2020 08:52:53 GMT

Protected "6zb6" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:52, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 6zb6 is ON HOLD until Paper Publication Authors: Papageorgiou, A.C., Poudel, N. Description: Crystal structure of Lolium rigidum GSTF in complex wi...

OCA — Wed, 17 Jun 2020 08:52:52 GMT

New page: '''Unreleased structure''' The entry 6zb6 is ON HOLD until Paper Publication Authors: Papageorgiou, A.C., Poudel, N. Description: Crystal structure of Lolium rigidum GSTF in complex wi...

New page

'''Unreleased structure'''

The entry 6zb6 is ON HOLD until Paper Publication

Authors: Papageorgiou, A.C., Poudel, N.

Description: Crystal structure of Lolium rigidum GSTF in complex with S-(p-nitrobenzyl) glutathione
[[Category: Unreleased Structures]]
[[Category: Papageorgiou, A.C]]
[[Category: Poudel, N]]