6zbk - Revision history

OCA at 13:45, 24 January 2024

OCA — Wed, 24 Jan 2024 13:45:50 GMT

←Older revision		Revision as of 13:45, 24 January 2024
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-	====	+	==Crystal structure of the human complex between RPAP3 and TRBP==
-	<StructureSection load='6zbk' size='340' side='right'caption='[[6zbk]]' scene=''>	+	<StructureSection load='6zbk' size='340' side='right'caption='[[6zbk]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zbk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZBK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zbk OCA], [https://pdbe.org/6zbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zbk RCSB], [https://www.ebi.ac.uk/pdbsum/6zbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zbk ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.49Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zbk OCA], [https://pdbe.org/6zbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zbk RCSB], [https://www.ebi.ac.uk/pdbsum/6zbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zbk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RPAP3_HUMAN RPAP3_HUMAN] Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation.<ref>PMID:17643375</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MicroRNAs silence mRNAs by guiding the RISC complex. RISC assembly occurs following cleavage of pre-miRNAs by Dicer, assisted by TRBP or PACT, and the transfer of miRNAs to AGO proteins. The R2TP complex is an HSP90 co-chaperone involved in the assembly of ribonucleoprotein particles. Here, we show that the R2TP component RPAP3 binds TRBP but not PACT. The RPAP3-TPR1 domain interacts with the TRBP-dsRBD3, and the 1.5 A resolution crystal structure of this complex identifies key residues involved in the interaction. Remarkably, binding of TRBP to RPAP3 or Dicer is mutually exclusive. Additionally, we found that AGO(1/2), TRBP and Dicer are all sensitive to HSP90 inhibition, and that TRBP sensitivity is increased in the absence of RPAP3. Finally, RPAP3 seems to impede miRNA activity, raising the possibility that the R2TP chaperone might sequester TRBP to regulate the miRNA pathway.
		+
		+	The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.,Abel Y, Charron C, Virciglio C, Bourguignon-Igel V, Quinternet M, Chagot ME, Robert MC, Verheggen C, Branlant C, Bertrand E, Manival X, Charpentier B, Rederstorff M Nucleic Acids Res. 2022 Feb 28;50(4):2172-2189. doi: 10.1093/nar/gkac086. PMID:35150569<ref>PMID:35150569</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zbk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Abel Y]]
		+	[[Category: Charpentier B]]
		+	[[Category: Charron C]]
		+	[[Category: Rederstorff M]]

OCA at 03:31, 2 July 2021

OCA — Fri, 02 Jul 2021 03:31:14 GMT

←Older revision		Revision as of 03:31, 2 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6zbk ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6zbk' size='340' side='right'caption='[[6zbk]]' scene=''>
-	~~Authors~~: ~~Charron, C~~.~~, Abel, Y~~.~~, Charpentier, B~~.~~, Rederstorff, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal~~ structure ~~of the human complexe between RPAP3 and TRBP~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zbk OCA], [https://pdbe.org/6zbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zbk RCSB], [https://www.ebi.ac.uk/pdbsum/6zbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zbk ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[[~~Category~~: ~~Rederstorff~~, M]]	+	__TOC__
-	[~~[Category~~: ~~Charron, C~~]]	+	</StructureSection>
-	[[Category: ~~Abel, Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Charpentier, B~~]]	+	[[Category: Z-disk]]

OCA at 18:35, 24 June 2020

OCA — Wed, 24 Jun 2020 18:35:35 GMT

←Older revision		Revision as of 18:35, 24 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zbk is ON HOLD	+	The entry 6zbk is ON HOLD until Paper Publication

	Authors: Charron, C., Abel, Y., Charpentier, B., Rederstorff, M.		Authors: Charron, C., Abel, Y., Charpentier, B., Rederstorff, M.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rederstorff, M]]		[[Category: Rederstorff, M]]
-	[[Category: Abel, Y]]
	[[Category: Charron, C]]		[[Category: Charron, C]]
		+	[[Category: Abel, Y]]
	[[Category: Charpentier, B]]		[[Category: Charpentier, B]]

OCA: Protected "6zbk" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2020 08:54:04 GMT

Protected "6zbk" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 6zbk is ON HOLD Authors: Charron, C., Abel, Y., Charpentier, B., Rederstorff, M. Description: Crystal structure of the human complexe between RPAP3...

OCA — Wed, 17 Jun 2020 08:54:03 GMT

New page: '''Unreleased structure''' The entry 6zbk is ON HOLD Authors: Charron, C., Abel, Y., Charpentier, B., Rederstorff, M. Description: Crystal structure of the human complexe between RPAP3...

New page

'''Unreleased structure'''

The entry 6zbk is ON HOLD

Authors: Charron, C., Abel, Y., Charpentier, B., Rederstorff, M.

Description: Crystal structure of the human complexe between RPAP3 and TRBP
[[Category: Unreleased Structures]]
[[Category: Rederstorff, M]]
[[Category: Abel, Y]]
[[Category: Charron, C]]
[[Category: Charpentier, B]]