6zc9 - Revision history

OCA at 13:46, 24 January 2024

OCA — Wed, 24 Jan 2024 13:46:30 GMT

←Older revision		Revision as of 13:46, 24 January 2024
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-	====	+	==Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 binding motif Ser448==
-	<StructureSection load='6zc9' size='340' side='right'caption='[[6zc9]]' scene=''>	+	<StructureSection load='6zc9' size='340' side='right'caption='[[6zc9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zc9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZC9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zc9 OCA], [https://pdbe.org/6zc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zc9 RCSB], [https://www.ebi.ac.uk/pdbsum/6zc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zc9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8989599Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFH:1,1,1,3,3,3-HEXAFLUOROPROPAN-2-OL'>CFH</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zc9 OCA], [https://pdbe.org/6zc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zc9 RCSB], [https://www.ebi.ac.uk/pdbsum/6zc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zc9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433G_HUMAN 1433G_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.<ref>PMID:16511572</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neural precursor cell expressed developmentally down-regulated 4 ligase (Nedd4-2) is an E3 ubiquitin ligase that targets proteins for ubiquitination and endocytosis, thereby regulating numerous ion channels, membrane receptors and tumor suppressors. Nedd4-2 activity is regulated by autoinhibition, calcium binding, oxidative stress, substrate binding, phosphorylation and 14-3-3 protein binding. However, the structural basis of 14-3-3-mediated Nedd4-2 regulation remains poorly understood. Here, we combined several techniques of integrative structural biology to characterize Nedd4-2 and its complex with 14-3-3. We demonstrate that phosphorylated Ser(342) and Ser(448) are the key residues that facilitate 14-3-3 protein binding to Nedd4-2 and that 14-3-3 protein binding induces a structural rearrangement of Nedd4-2 by inhibiting interactions between its structured domains. Overall, our findings provide the structural glimpse into the 14-3-3-mediated Nedd4-2 regulation and highlight the potential of the Nedd4-2:14-3-3 complex as a pharmacological target for Nedd4-2-associated diseases such as hypertension, epilepsy, kidney disease and cancer.
		+
		+	14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains.,Pohl P, Joshi R, Petrvalska O, Obsil T, Obsilova V Commun Biol. 2021 Jul 22;4(1):899. doi: 10.1038/s42003-021-02419-0. PMID:34294877<ref>PMID:34294877</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zc9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kalabova D]]
		+	[[Category: Obsil T]]
		+	[[Category: Obsilova V]]
		+	[[Category: Pohl P]]

OCA at 08:55, 21 July 2021

OCA — Wed, 21 Jul 2021 08:55:52 GMT

←Older revision		Revision as of 08:55, 21 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6zc9 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6zc9' size='340' side='right'caption='[[6zc9]]' scene=''>
-	~~Authors~~: ~~Pohl, P~~.~~, Kalabova, D~~.~~, Obsil, T~~.~~, Obsilova, V~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 binding motif Ser448~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zc9 OCA], [https://pdbe.org/6zc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zc9 RCSB], [https://www.ebi.ac.uk/pdbsum/6zc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zc9 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Obsil~~, T]]	+	__TOC__
-	[~~[Category~~: ~~Obsilova~~, V]]	+	</StructureSection>
-	[[Category: ~~Pohl, P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kalabova, D~~]]	+	[[Category: Z-disk]]

OCA at 07:53, 22 July 2020

OCA — Wed, 22 Jul 2020 07:53:08 GMT

←Older revision		Revision as of 07:53, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zc9 is ON HOLD	+	The entry 6zc9 is ON HOLD until Paper Publication

	Authors: Pohl, P., Kalabova, D., Obsil, T., Obsilova, V.		Authors: Pohl, P., Kalabova, D., Obsil, T., Obsilova, V.

OCA: Protected "6zc9" [edit=sysop:move=sysop]

OCA — Wed, 17 Jun 2020 08:56:23 GMT

Protected "6zc9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:56, 17 June 2020

OCA: New page: '''Unreleased structure''' The entry 6zc9 is ON HOLD Authors: Pohl, P., Kalabova, D., Obsil, T., Obsilova, V. Description: Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 bind...

OCA — Wed, 17 Jun 2020 08:56:21 GMT

New page: '''Unreleased structure''' The entry 6zc9 is ON HOLD Authors: Pohl, P., Kalabova, D., Obsil, T., Obsilova, V. Description: Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 bind...

New page

'''Unreleased structure'''

The entry 6zc9 is ON HOLD

Authors: Pohl, P., Kalabova, D., Obsil, T., Obsilova, V.

Description: Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 binding motif Ser448
[[Category: Unreleased Structures]]
[[Category: Obsil, T]]
[[Category: Obsilova, V]]
[[Category: Pohl, P]]
[[Category: Kalabova, D]]