6zei - Revision history

OCA at 13:47, 24 January 2024

OCA — Wed, 24 Jan 2024 13:47:54 GMT

←Older revision		Revision as of 13:47, 24 January 2024
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-	====	+	==Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)==
-	<StructureSection load='6zei' size='340' side='right'caption='[[6zei]]' scene=''>	+	<StructureSection load='6zei' size='340' side='right'caption='[[6zei]], [[Resolution\|resolution]] 1.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zei]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZEI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zei OCA], [~~http~~://pdbe.org/6zei PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zei RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zei PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zei ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.39Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zei OCA], [https://pdbe.org/6zei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zei RCSB], [https://www.ebi.ac.uk/pdbsum/6zei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zei ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PP1A_HUMAN PP1A_HUMAN] Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development.<ref>PMID:17283141</ref> [https://www.uniprot.org/uniprot/BAIP2_HUMAN BAIP2_HUMAN] Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:11130076</ref> <ref>PMID:11696321</ref> <ref>PMID:14752106</ref> <ref>PMID:19366662</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PPP-family phosphatases such as PP1 have little intrinsic specificity. Cofactors can target PP1 to substrates or subcellular locations, but it remains unclear how they might confer sequence-specificity on PP1. The cytoskeletal regulator Phactr1 is a neuronally-enriched PP1 cofactor that is controlled by G-actin. Structural analysis showed that Phactr1 binding remodels PP1's hydrophobic groove, creating a new composite surface adjacent to the catalytic site. Using phosphoproteomics, we identified mouse fibroblast and neuronal Phactr1/PP1 substrates, which include cytoskeletal components and regulators. We determined high-resolution structures of Phactr1/PP1 bound to the dephosphorylated forms of its substrates IRSp53 and spectrin aII. Inversion of the phosphate in these holoenzyme-product complexes supports the proposed PPP-family catalytic mechanism. Substrate sequences C-terminal to the dephosphorylation site make intimate contacts with the composite Phactr1/PP1 surface, which are required for efficient dephosphorylation. Sequence specificity explains why Phactr1/PP1 exhibits orders-of-magnitude enhanced reactivity towards its substrates, compared to apo-PP1 or other PP1 holoenzymes.
		+
		+	Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme.,Fedoryshchak RO, Prechova M, Butler A, Lee R, O'Reilly N, Flynn HR, Snijders AP, Eder N, Ultanir S, Mouilleron S, Treisman R Elife. 2020 Sep 25;9. pii: 61509. doi: 10.7554/eLife.61509. PMID:32975518<ref>PMID:32975518</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zei" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Butler AM]]
		+	[[Category: Fedoryshchak R]]
		+	[[Category: Lee R]]
		+	[[Category: Mouilleron S]]
		+	[[Category: Prechova M]]
		+	[[Category: Treisman R]]

OCA at 07:12, 30 September 2020

OCA — Wed, 30 Sep 2020 07:12:26 GMT

←Older revision		Revision as of 07:12, 30 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6zei ~~is ON HOLD until Jun 16 2022~~	+	====
-		+	<StructureSection load='6zei' size='340' side='right'caption='[[6zei]]' scene=''>
-	~~Authors~~: ~~Mouilleron, S~~.~~, Treisman, R~~.~~, Fedoryshchak, R~~.~~, Lee, R~~.~~, Butler, A~~.M.~~, Prechova, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of PP1-IRSp53 S455E chimera~~ [~~PP1(7-304) + linker (G~~/~~S)x9 + IRSp53(449-465)~~] ~~bound to Phactr1 (516-580)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zei OCA], [http://pdbe.org/6zei PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zei RCSB], [http://www.ebi.ac.uk/pdbsum/6zei PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zei ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Lee~~, ~~R]]~~	+	__TOC__
-	[~~[Category~~: ~~Fedoryshchak, R]~~]	+	</StructureSection>
-	[~~[Category~~: ~~Butler, A~~.M]]	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Prechova, M]~~]	+	[[Category: Z-disk]]
-	[[Category: ~~Treisman, R~~]]	+
-	[[Category: ~~Mouilleron, S~~]]	+

OCA at 07:56, 22 July 2020

OCA — Wed, 22 Jul 2020 07:56:30 GMT

←Older revision		Revision as of 07:56, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6zei is ON HOLD	+	The entry 6zei is ON HOLD until Jun 16 2022

	Authors: Mouilleron, S., Treisman, R., Fedoryshchak, R., Lee, R., Butler, A.M., Prechova, M.		Authors: Mouilleron, S., Treisman, R., Fedoryshchak, R., Lee, R., Butler, A.M., Prechova, M.
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	Description: Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)		Description: Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Treisman, R]]
-	[[Category: Mouilleron, S]]
	[[Category: Lee, R]]		[[Category: Lee, R]]
	[[Category: Fedoryshchak, R]]		[[Category: Fedoryshchak, R]]
	[[Category: Butler, A.M]]		[[Category: Butler, A.M]]
	[[Category: Prechova, M]]		[[Category: Prechova, M]]
		+	[[Category: Treisman, R]]
		+	[[Category: Mouilleron, S]]

OCA: Protected "6zei" [edit=sysop:move=sysop]

OCA — Wed, 24 Jun 2020 18:41:54 GMT

Protected "6zei" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:41, 24 June 2020

OCA: New page: '''Unreleased structure''' The entry 6zei is ON HOLD Authors: Mouilleron, S., Treisman, R., Fedoryshchak, R., Lee, R., Butler, A.M., Prechova, M. Description: Structure of PP1-IRSp53 S...

OCA — Wed, 24 Jun 2020 18:41:53 GMT

New page: '''Unreleased structure''' The entry 6zei is ON HOLD Authors: Mouilleron, S., Treisman, R., Fedoryshchak, R., Lee, R., Butler, A.M., Prechova, M. Description: Structure of PP1-IRSp53 S...

New page

'''Unreleased structure'''

The entry 6zei is ON HOLD

Authors: Mouilleron, S., Treisman, R., Fedoryshchak, R., Lee, R., Butler, A.M., Prechova, M.

Description: Structure of PP1-IRSp53 S455E chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)
[[Category: Unreleased Structures]]
[[Category: Treisman, R]]
[[Category: Mouilleron, S]]
[[Category: Lee, R]]
[[Category: Fedoryshchak, R]]
[[Category: Butler, A.M]]
[[Category: Prechova, M]]