OCA at 10:22, 16 September 2020

OCA — Wed, 16 Sep 2020 10:22:52 GMT

←Older revision		Revision as of 10:22, 16 September 2020
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	==Furin Cleaved Spike Protein of SARS-CoV-2 with One RBD Erect==		==Furin Cleaved Spike Protein of SARS-CoV-2 with One RBD Erect==
-	<StructureSection load='6zgg' size='340' side='right'caption='[[6zgg]]' scene=''>	+	<StructureSection load='6zgg' size='340' side='right'caption='[[6zgg]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZGG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zgg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZGG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgg OCA], [http://pdbe.org/6zgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zgg RCSB], [http://www.ebi.ac.uk/pdbsum/6zgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgg ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S, 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgg OCA], [http://pdbe.org/6zgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zgg RCSB], [http://www.ebi.ac.uk/pdbsum/6zgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2]] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SARS-CoV-2 is thought to have emerged from bats, possibly via a secondary host. Here, we investigate the relationship of spike (S) glycoprotein from SARS-CoV-2 with the S protein of a closely related bat virus, RaTG13. We determined cryo-EM structures for RaTG13 S and for both furin-cleaved and uncleaved SARS-CoV-2 S; we compared these with recently reported structures for uncleaved SARS-CoV-2 S. We also biochemically characterized their relative stabilities and affinities for the SARS-CoV-2 receptor ACE2. Although the overall structures of human and bat virus S proteins are similar, there are key differences in their properties, including a more stable precleavage form of human S and about 1,000-fold tighter binding of SARS-CoV-2 to human receptor. These observations suggest that cleavage at the furin-cleavage site decreases the overall stability of SARS-CoV-2 S and facilitates the adoption of the open conformation that is required for S to bind to the ACE2 receptor.

-	~~==See Also==~~	+	SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.,Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ Nat Struct Mol Biol. 2020 Jul 9. pii: 10.1038/s41594-020-0468-7. doi:, 10.1038/s41594-020-0468-7. PMID:32647346<ref>PMID:32647346</ref>
-	*[[SARS-CoV-2 ~~protein S activation by~~ furin~~\|SARS~~-~~CoV~~-~~2 protein~~ S ~~activation by furin]]~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zgg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: 2019-ncov]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Benton DJ]]	+	[[Category: Benton, D J]]
-	[[Category: Gamblin SJ]]	+	[[Category: Gamblin, S J]]
-	[[Category: Rosenthal PB]]	+	[[Category: Rosenthal, P B]]
-	[[Category: Wrobel AG]]	+	[[Category: Wrobel, A G]]
		+	[[Category: Coronavirus]]
		+	[[Category: Sars-cov-2]]
		+	[[Category: Spike]]
		+	[[Category: Viral protein]]
		+	[[Category: Virus glycoprotein]]

OCA at 03:41, 25 July 2020

OCA — Sat, 25 Jul 2020 03:41:07 GMT

←Older revision		Revision as of 03:41, 25 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6zgg is ~~ON HOLD~~	+	==Furin Cleaved Spike Protein of SARS-CoV-2 with One RBD Erect==
		+	<StructureSection load='6zgg' size='340' side='right'caption='[[6zgg]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZGG FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgg OCA], [http://pdbe.org/6zgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zgg RCSB], [http://www.ebi.ac.uk/pdbsum/6zgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgg ProSAT]</span></td></tr>
		+	</table>

-	~~Authors: Wrobel, A.G., Benton, D.J., Rosenthal, P.B., Gamblin, S.J.~~	+	==See Also==
-		+	*[[SARS-CoV-2 protein S activation by furin\|SARS-CoV-2 protein S activation by furin]]
-	~~Description: Furin Cleaved Spike Protein of~~ SARS-CoV-2 ~~with One RBD Erect~~	+	__TOC__
-	[[Category: ~~Unreleased~~ Structures]]	+	</StructureSection>
-	[[Category: ~~Rosenthal, P.B~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Benton, D.J~~]]	+	[[Category: Benton DJ]]
-	[[Category: ~~Wrobel, A.G~~]]	+	[[Category: Gamblin SJ]]
-	[[Category: ~~Gamblin, S.J~~]]	+	[[Category: Rosenthal PB]]
		+	[[Category: Wrobel AG]]

OCA: Protected "6zgg" [edit=sysop:move=sysop]

OCA — Wed, 24 Jun 2020 18:48:07 GMT

Protected "6zgg" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:48, 24 June 2020

OCA: New page: '''Unreleased structure''' The entry 6zgg is ON HOLD Authors: Wrobel, A.G., Benton, D.J., Rosenthal, P.B., Gamblin, S.J. Description: Furin Cleaved Spike Protein of SARS-CoV-2 with One...

OCA — Wed, 24 Jun 2020 18:48:06 GMT

New page: '''Unreleased structure''' The entry 6zgg is ON HOLD Authors: Wrobel, A.G., Benton, D.J., Rosenthal, P.B., Gamblin, S.J. Description: Furin Cleaved Spike Protein of SARS-CoV-2 with One...

New page

'''Unreleased structure'''

The entry 6zgg is ON HOLD

Authors: Wrobel, A.G., Benton, D.J., Rosenthal, P.B., Gamblin, S.J.

Description: Furin Cleaved Spike Protein of SARS-CoV-2 with One RBD Erect
[[Category: Unreleased Structures]]
[[Category: Rosenthal, P.B]]
[[Category: Benton, D.J]]
[[Category: Wrobel, A.G]]
[[Category: Gamblin, S.J]]

6zgg - Revision history

OCA at 10:22, 16 September 2020

OCA at 03:41, 25 July 2020

OCA: Protected "6zgg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6zgg is ON HOLD Authors: Wrobel, A.G., Benton, D.J., Rosenthal, P.B., Gamblin, S.J. Description: Furin Cleaved Spike Protein of SARS-CoV-2 with One...