6zj5 - Revision history

OCA at 11:53, 1 February 2024

OCA — Thu, 01 Feb 2024 11:53:16 GMT

←Older revision		Revision as of 11:53, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='6zj5' size='340' side='right'caption='[[6zj5]], [[Resolution\|resolution]] 2.27Å' scene=''>		<StructureSection load='6zj5' size='340' side='right'caption='[[6zj5]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6zj5]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ZJ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zj5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZJ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMJ:1-DEOXYMANNOJIRIMYCIN'>DMJ</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.269Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MANEA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMJ:1-DEOXYMANNOJIRIMYCIN'>DMJ</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycoprotein_endo-alpha-1,2-mannosidase Glycoprotein endo-alpha-1,2-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.130 3.2.1.130] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj5 OCA], [https://pdbe.org/6zj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zj5 RCSB], [https://www.ebi.ac.uk/pdbsum/6zj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj5 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj5 OCA], [~~http~~://pdbe.org/6zj5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zj5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zj5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj5 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MANEA_HUMAN MANEA_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6zj5" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6zj5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mannosidase 3D structures\|Mannosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glycoprotein endo-alpha-1,2-mannosidase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alonzi~~, D S~~]]	+	[[Category: Alonzi DS]]
-	[[Category: Butters~~, T D~~]]	+	[[Category: Butters TD]]
-	[[Category: Davies~~, G J~~]]	+	[[Category: Davies GJ]]
-	[[Category: Davies, S]]	+	[[Category: Davies S]]
-	[[Category: Fernandes~~, P Z~~]]	+	[[Category: Fernandes PZ]]
-	[[Category: Hakki, Z]]	+	[[Category: Hakki Z]]
-	[[Category: Hill, M]]	+	[[Category: Hill M]]
-	[[Category: Howe~~, J D~~]]	+	[[Category: Howe JD]]
-	[[Category: Sobala~~, L F~~]]	+	[[Category: Sobala LF]]
-	[[Category: Stamataki, Z]]	+	[[Category: Stamataki Z]]
-	[[Category: Thompson~~, A J~~]]	+	[[Category: Thompson AJ]]
-	[[Category: Williams~~, S J~~]]	+	[[Category: Williams SJ]]
-	[[Category: Zitzmann, N]]	+	[[Category: Zitzmann N]]
-	~~[[Category: Golgi]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mannosidase]]~~	+
-	~~[[Category: Retaining~~]]	+

OCA at 08:27, 20 January 2021

OCA — Wed, 20 Jan 2021 08:27:00 GMT

←Older revision		Revision as of 08:27, 20 January 2021
Line 1:		Line 1:

	==Structure of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with GlcDMJ and hexatungstotellurate(VI) TEW==		==Structure of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with GlcDMJ and hexatungstotellurate(VI) TEW==
-	<StructureSection load='6zj5' size='340' side='right'caption='[[6zj5]]' scene=''>	+	<StructureSection load='6zj5' size='340' side='right'caption='[[6zj5]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZJ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zj5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZJ5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj5 OCA], [http://pdbe.org/6zj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zj5 RCSB], [http://www.ebi.ac.uk/pdbsum/6zj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj5 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMJ:1-DEOXYMANNOJIRIMYCIN'>DMJ</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MANEA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycoprotein_endo-alpha-1,2-mannosidase Glycoprotein endo-alpha-1,2-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.130 3.2.1.130] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj5 OCA], [http://pdbe.org/6zj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zj5 RCSB], [http://www.ebi.ac.uk/pdbsum/6zj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj5 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian protein N-linked glycosylation is critical for glycoprotein folding, quality control, trafficking, recognition, and function. N-linked glycans are synthesized from Glc3Man9GlcNAc2 precursors that are trimmed and modified in the endoplasmic reticulum (ER) and Golgi apparatus by glycoside hydrolases and glycosyltransferases. Endo-alpha-1,2-mannosidase (MANEA) is the sole endo-acting glycoside hydrolase involved in N-glycan trimming and is located within the Golgi, where it allows ER-escaped glycoproteins to bypass the classical N-glycosylation trimming pathway involving ER glucosidases I and II. There is considerable interest in the use of small molecules that disrupt N-linked glycosylation as therapeutic agents for diseases such as cancer and viral infection. Here we report the structure of the catalytic domain of human MANEA and complexes with substrate-derived inhibitors, which provide insight into dynamic loop movements that occur on substrate binding. We reveal structural features of the human enzyme that explain its substrate preference and the mechanistic basis for catalysis. These structures have inspired the development of new inhibitors that disrupt host protein N-glycan processing of viral glycans and reduce the infectivity of bovine viral diarrhea and dengue viruses in cellular models. These results may contribute to efforts aimed at developing broad-spectrum antiviral agents and help provide a more in-depth understanding of the biology of mammalian glycosylation.
		+
		+	Structure of human endo-alpha-1,2-mannosidase (MANEA), an antiviral host-glycosylation target.,Sobala LF, Fernandes PZ, Hakki Z, Thompson AJ, Howe JD, Hill M, Zitzmann N, Davies S, Stamataki Z, Butters TD, Alonzi DS, Williams SJ, Davies GJ Proc Natl Acad Sci U S A. 2020 Nov 5. pii: 2013620117. doi:, 10.1073/pnas.2013620117. PMID:33154157<ref>PMID:33154157</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zj5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Glycoprotein endo-alpha-1,2-mannosidase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alonzi DS]]	+	[[Category: Alonzi, D S]]
-	[[Category: Butters TD]]	+	[[Category: Butters, T D]]
-	[[Category: Davies GJ]]	+	[[Category: Davies, G J]]
-	[[Category: Davies S]]	+	[[Category: Davies, S]]
-	[[Category: Fernandes PZ]]	+	[[Category: Fernandes, P Z]]
-	[[Category: Hakki Z]]	+	[[Category: Hakki, Z]]
-	[[Category: Hill M]]	+	[[Category: Hill, M]]
-	[[Category: Howe JD]]	+	[[Category: Howe, J D]]
-	[[Category: Sobala LF]]	+	[[Category: Sobala, L F]]
-	[[Category: Stamataki Z]]	+	[[Category: Stamataki, Z]]
-	[[Category: Thompson AJ]]	+	[[Category: Thompson, A J]]
-	[[Category: Williams SJ]]	+	[[Category: Williams, S J]]
-	[[Category: Zitzmann N]]	+	[[Category: Zitzmann, N]]
		+	[[Category: Golgi]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mannosidase]]
		+	[[Category: Retaining]]

OCA at 06:38, 9 December 2020

OCA — Wed, 09 Dec 2020 06:38:45 GMT

←Older revision		Revision as of 06:38, 9 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6zj5 ~~is ON HOLD~~	+	==Structure of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with GlcDMJ and hexatungstotellurate(VI) TEW==
-		+	<StructureSection load='6zj5' size='340' side='right'caption='[[6zj5]]' scene=''>
-	~~Authors~~: ~~Sobala, L~~.F.~~, Fernandes, P~~.Z.~~, Hakki, Z~~.~~, Thompson, A~~.J.~~, Howe, J~~.D., ~~Hill, M~~.~~, Zitzmann, N~~.~~, Davies, S~~., ~~Stamataki, Z~~., ~~Butters, T~~.D., ~~Alonzi, D~~.S.~~, Williams, S.J~~., ~~Davies, G~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZJ5 FirstGlance]. <br>
-	~~Description: Structure of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with GlcDMJ and hexatungstotellurate(VI) TEW~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zj5 OCA], [http://pdbe.org/6zj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zj5 RCSB], [http://www.ebi.ac.uk/pdbsum/6zj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zj5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Zitzmann, N~~]]	+	__TOC__
-	[[Category: ~~Alonzi, D.S~~]]	+	</StructureSection>
-	[[Category: Davies~~, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Stamataki, Z~~]]	+	[[Category: Alonzi DS]]
-	[[Category: ~~Williams, S.J~~]]	+	[[Category: Butters TD]]
-	[[Category: ~~Howe, J.D~~]]	+	[[Category: Davies GJ]]
-	[[Category: Hill, M]]	+	[[Category: Davies S]]
-	[[Category: ~~Thompson, A.J~~]]	+	[[Category: Fernandes PZ]]
-	[[Category: ~~Davies, G.J~~]]	+	[[Category: Hakki Z]]
-	[[Category: ~~Hakki,~~ Z]]	+	[[Category: Hill M]]
-	[[Category: ~~Sobala, L.F~~]]	+	[[Category: Howe JD]]
-	[[Category: ~~Fernandes, P.Z~~]]	+	[[Category: Sobala LF]]
-	[[Category: ~~Butters, T.D~~]]	+	[[Category: Stamataki Z]]
		+	[[Category: Thompson AJ]]
		+	[[Category: Williams SJ]]
		+	[[Category: Zitzmann N]]

OCA: Protected "6zj5" [edit=sysop:move=sysop]

OCA — Wed, 08 Jul 2020 11:13:04 GMT

Protected "6zj5" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:13, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6zj5 is ON HOLD Authors: Sobala, L.F., Fernandes, P.Z., Hakki, Z., Thompson, A.J., Howe, J.D., Hill, M., Zitzmann, N., Davies, S., Stamataki, Z., Bu...

OCA — Wed, 08 Jul 2020 11:13:03 GMT

New page: '''Unreleased structure''' The entry 6zj5 is ON HOLD Authors: Sobala, L.F., Fernandes, P.Z., Hakki, Z., Thompson, A.J., Howe, J.D., Hill, M., Zitzmann, N., Davies, S., Stamataki, Z., Bu...

New page

'''Unreleased structure'''

The entry 6zj5 is ON HOLD

Authors: Sobala, L.F., Fernandes, P.Z., Hakki, Z., Thompson, A.J., Howe, J.D., Hill, M., Zitzmann, N., Davies, S., Stamataki, Z., Butters, T.D., Alonzi, D.S., Williams, S.J., Davies, G.J.

Description: Structure of the catalytic domain of human endo-alpha-mannosidase MANEA in complex with GlcDMJ and hexatungstotellurate(VI) TEW
[[Category: Unreleased Structures]]
[[Category: Zitzmann, N]]
[[Category: Alonzi, D.S]]
[[Category: Davies, S]]
[[Category: Stamataki, Z]]
[[Category: Williams, S.J]]
[[Category: Howe, J.D]]
[[Category: Hill, M]]
[[Category: Thompson, A.J]]
[[Category: Davies, G.J]]
[[Category: Hakki, Z]]
[[Category: Sobala, L.F]]
[[Category: Fernandes, P.Z]]
[[Category: Butters, T.D]]